61 research outputs found
Chlorin Index: A new parameter for organic matter freshness in sediments
Total chlorins, comprising degradation products of chlorophyll, have been used recently to reconstruct paleoproductivity from marine sediment cores. Here, we report on a new index, the Chlorin Index (CI), that proves to be a helpful tool for rapidly estimating organic matter freshness in marine sediments. The CI is a ratio between the fluorescence intensity of a sediment extracted with acetone and treated with hydrochloric acid and the original sediment extract. It represents the ratio of chlorophyll and its degradation products deposited in the sediments that could still be chemically transformed and those that are inert to chemical attack. The ratio is lower in sediments that include freshly deposited phytoplankton material and higher in older, more degraded sediments. We measured this new parameter on surface sediments, and sediments from several short and a long sediment core from different oceanic settings. CI values range from 0.2 for chlorophyll a to 0.36–0.56 for fresh material deposited on the shelf off Namibia to values around 0.67 in sediments off Chile and Peru to values up to 0.97 for sediments in a deep core from the northeastern slope of the Arabian Sea. We have compared the CI to rates of bacterial sulfate reduction, as a direct measure of organic matter reactivity and to other degradation indices based on amino acid composition. We conclude that the CI is a reliable and simple tool for the characterization of organic material freshness in sediments in respect to its degradation state
Turnover of microbial lipids in the deep biosphere and growth of benthic archaeal populations
Deep subseafloor sediments host a microbial biosphere with unknown impact on global biogeochemical cycles. This study tests previous evidence based on microbial intact polar lipids (IPLs) as proxies of live biomass, suggesting that Archaea dominate the marine sedimentary biosphere: We devised a sensitive radiotracer assay to measure the decay rate of ([C-14]glucosyl)-diphytanylglyceroldiether (GlcDGD) as an analog of archaeal IPLs in continental margin sediments. The degradation kinetics were incorporated in model simulations that constrained the fossil fraction of subseafloor IPLs and rates of archaeal turnover. Simulating the top 1 km in a generic continental margin sediment column, we estimated degradation rate constants of GlcDGD being one to two orders of magnitude lower than those of bacterial IPLs, with half-lives of GlcDGD increasing with depth to 310 ky. Given estimated microbial community turnover times of 1.6-73 ky in sediments deeper than 1 m, 50-96% of archaeal IPLs represent fossil signals. Consequently, previous lipid-based estimates of global subseafloor biomass probably are too high, and the widely observed dominance of archaeal IPLs does not rule out a deep biosphere dominated by Bacteria. Reverse modeling of existing concentration profiles suggest that archaeal IPL synthesis rates decline from around 1,000 pg.mL(-1) sediment.y(-1) at the surface to 0.2 pg.mL(-1).y(-1) at 1 km depth, equivalent to production of 7 x 10(5) to 140 archaeal cells.mL(-1) sediment.y(-1), respectively. These constraints on microbial growth are an important step toward understanding the relationship between the deep biosphere and the carbon cycle
Microbial conversion of inorganic carbon to dimethyl sulfide in anoxic lake sediment (Plußsee, Germany)
In anoxic environments, volatile methylated sulfides like methanethiol (MT) and dimethyl sulfide (DMS) link the pools of inorganic and organic carbon with the sulfur cycle. However, direct formation of methylated sulfides from reduction of dissolved inorganic carbon has previously not been demonstrated. When studying the effect of temperature on hydrogenotrophic microbial activity, we observed formation of DMS in anoxic sediment of Lake Plußsee at 55 °C. Subsequent experiments strongly suggested that the formation of DMS involves fixation of bicarbonate via a reductive pathway in analogy to methanogenesis and engages methylation of MT. DMS formation was enhanced by addition of bicarbonate and further increased when both bicarbonate and H<sub>2</sub> were supplemented. Inhibition of DMS formation by 2-bromoethanesulfonate points to the involvement of methanogens. Compared to the accumulation of DMS, MT showed the opposite trend but there was no apparent 1:1 stoichiometric ratio between both compounds. Both DMS and MT had negative &delta;<sup>13</sup>C values of −62&permil; and −55&permil;, respectively. Labeling with NaH<sup>13</sup>CO<sub>3</sub> showed more rapid incorporation of bicarbonate into DMS than into MT. The stable carbon isotopic evidence implies that bicarbonate was fixed via a reductive pathway of methanogenesis, and the generated methyl coenzyme M became the methyl donor for MT methylation. Neither DMS nor MT accumulation were stimulated by addition of the methyl-group donors methanol and syringic acid or by the methyl-group acceptor hydrogen sulphide. The source of MT was further investigated in a H<sub>2</sub><sup>35</sup>S labeling experiment, which demonstrated a microbially-mediated process of hydrogen sulfide methylation to MT that accounted for only <10% of the accumulation rates of DMS. Therefore, the major source of the <sup>13</sup>C-depleted MT was neither bicarbonate nor methoxylated aromatic compounds. Other possibilities for isotopically depleted MT, such as other organic precursors like methionine, are discussed. This DMS-forming pathway may be relevant for anoxic environments such as hydrothermally influenced sediments and fluids and sulfate-methane transition zones in marine sediments
The Deep Sea and Sub-Seafloor Frontier
The deep sea and its sub-seafloor contain a vast reservoir of physical, mineral and biological resources that are rapidly coming into the window of exploitation. Assessing
the opportunities and the risks involved requires a serious commitment to excellent deep sea research.
There are numerous areas in this field in which Europe has cutting-edge technological
potential. These include drilling and monitoring technology in the field of
renewable energies such as geothermal, offshore wind and seafloor resources.
Scientific ocean drilling will continue to play a valuable role, for example in the
exploration of resource opportunities, in obtaining estimates for ecosystem and Earth climate sensitivity, or in improving understanding about the controlling factors governing processes and recurrence
intervals of submarine geohazards.
In Europe, there is also the scientific expertise needed to define a framework for policymakers for environmental protection measures and to carry out ecological impact
assessments before, during and after commercial exploitation. Taking up these societal challenges will strengthen European scientific and educational networks and
promote the development of world-class technology and industrial leadership.Published3.7. Dinamica del clima e dell'oceano4.6. Oceanografia operativa per la valutazione dei rischi in aree marineope
Niche partitioning by photosynthetic plankton as a driver of CO2-fixation across the oligotrophic South Pacific Subtropical Ocean
Oligotrophic ocean gyre ecosystems may be expanding due to rising global temperatures [1-5]. Models predicting carbon flow through these changing ecosystems require accurate descriptions of phytoplankton communities and their metabolic activities [6]. We therefore measured distributions and activities of cyanobacteria and small photosynthetic eukaryotes throughout the euphotic zone on a zonal transect through the South Pacific Ocean, focusing on the ultraoligotrophic waters of the South Pacific Gyre (SPG). Bulk rates of CO2 fixation were low (0.1 mu mol Cl--(1) d(-1)) but pervasive throughout both the surface mixed-layer (upper 150 m), as well as the deep chlorophyll a maximum of the core SPG. Chloroplast 16S rRNA metabarcoding, and single-cell (CO2)-C-13 uptake experiments demonstrated niche differentiation among the small eukaryotes and picocyanobacteria. Prochlorococcus abundances, activity, and growth were more closely associated with the rims of the gyre. Small, fast-growing, photosynthetic eukaryotes, likely related to the Pelagophyceae, characterized the deep chlorophyll a maximum. In contrast, a slower growing population of photosynthetic eukaryotes, likely comprised of Dictyochophyceae and Chrysophyceae, dominated the mixed layer that contributed 65-88% of the areal CO2 fixation within the core SPG. Small photosynthetic eukaryotes may thus play an underappreciated role in CO2 fixation in the surface mixed-layer waters of ultraoligotrophic ecosystems
Effect of the aerenchymatous helophyte Glyceria maxima on the sulfate-reducing communities in two contrasting riparian grassland soils
Shelfbreak frontal structure on the continental shelf north of Cape Hatteras
The continental shelf circulation north of Cape Hatteras is strongly affected by the proximity of the Gulf Stream. Previous observations have shown that this is an area in which Middle Atlantic Eight sub-pycnocline (''cold pool'') shelf water flows offshore, directly across isobaths, eventually becoming entrained in the north wall of the Gulf Stream. Hydrographic observations from the summers of 1990 and 1991 are used to show two different scenarios for the interaction of Gulf Stream water with the shelf flow. A high resolution cross-shelf transect was also sampled off New Jersey in each year to provide contrasting frontal structure well north of Cape Hatteras. In 1991 off New Jersey, shipboard velocity measurements indicated a baroclinic jet at the shelfbreak front, with a maximum along-shelf velocity of 0.5 m s(-1). Large cross-frontal density gradients were located near both the bottom outcrop of the front as well as in the seasonal pycnocline, resulting in two distinct velocity maxima, one above and one below the seasonal pycnocline. The latter was associated with a pycnocline salinity maximum extending from the continental slope shoreward across the shelfbreak. The frontal structure near Cape Hatteras was very different, due to the presence of Gulf Stream water over the continental shelf and slope. In June 1990, the shelf water was diverted offshore 100 km north of Cape Hatteras, with Gulf Stream water present over the shelf farther south. Estimated geostrophic eastward velocities at the 1000-m isobath were 0.2 m s(-1) (relative to 100 m). In 1991, Gulf Stream water was present over the upper slope, abutting the shelf water, but was not present over the shelf during the brief (3-day) survey. However, the near-surface Gulf Stream water over the upper slope was less dense than the shelf water and drove a northward flow of 0.2 m s(-1) in the upper 30 m of the water column. In both cases, large along-isobath density gradients were present at the 1000-m isobath, consistent with geostrophic offshore flows of up to 0.2 m s(-1). Thus, the presence of Gulf Stream water may affect the shelf circulation north of Cape Hatteras in at least two different ways: by either flooding the shelf and diverting the southwestward flow of water from the Middle Atlantic Eight directly offshore well north of Cape Hatteras, or by reversing the density gradients normally associated with the shelfbreak front and potentially allowing the shelf water to move offshore along downward-sloping isopycnals. Copyright (C) 1996 Elsevier Science Ltd
High-Pressure Systems for Gas-Phase Free Continuous Incubation of Enriched Marine Microbial Communities Performing Anaerobic High-Pressure Systems for Gas-Phase Free Continuous Incubation of Enriched Marine Microbial Communities Performing Anaerobic Oxidation of Methane
Novel high-pressure biotechnical systems that were developed and applied for the study of anaerobic oxidation of methane (AOM) are described. The systems, referred to as high-pressure continuous incubation system (HP-CI system) and high-pressure manifold-incubation system (HP-MI system), allow for batch, fed-batch, and continuous gas-phase free incubation at high concentrations of dissolved methane and were designed to meet specific demands for studying environmental regulation and kinetics as well as for enriching microbial biomass in long-term incubation. Anoxic medium is saturated with methane in the first technical stage, and the saturated medium is supplied for biomass incubation in the second stage. Methane can be provided in continuous operation up to 20 MPa and the incubation systems can be operated during constant supply of gas-enriched medium at a hydrostatic pressure up to 45 MPa. To validate the suitability of the high-pressure systems, we present data from continuous and fed-batch incubation of highly active samples prepared from microbial mats from the Black Sea collected at a water depth of 213 m. In continuous operation in the HP-CI system initial methane-dependent sulfide production was enhanced 10- to 15-fold after increasing the methane partial pressure from near ambient pressure of 0.2 to 10.0 MPa at a hydrostatic pressure of 16.0 MPa in the incubation stage. With a hydraulic retention time of 14 h a stable effluent sulfide concentration was reached within less than 3 days and a continuing increase of the volumetric AOM rate from 1.2 to 1.7 mmol L−1 day−1 was observed over 14 days. In fed-batch incubation the AOM rate increased from 1.5 to 2.7 and 3.6 mmol L−1 day−1 when the concentration of aqueous methane was stepwise increased from 5 to 15 mmol L−1 and 45 mmol L−1. A methane partial pressure of 6 MPa and a hydrostatic pressure of 12 MPa in manifold fed-batch incubation in the HP-MI system yielded a sixfold increase in the volumetric AOM rate. Over subsequent incubation periods AOM rates increased from 0.6 to 1.2 mmol L−1 day−1 within 26 days of incubation. No inhibition of biomass activity was observed in all continuous and fed-batch incubation experiments. The organisms were able to tolerate high sulfide concentrations and extended starvation periods
Oxygen penetration deep into the sediment of the South Pacific gyre
Sediment oxygen concentration profiles and benthic microbial oxygen consumption rates were investigated during an IODP site survey in the South Pacific Gyre. Primary production, particle fluxes and sedimentation rates are extremely low in this ultra-oligotrophic oceanic region. We derived O2 consumption rates from vertical oxygen profiles in sediments obtained on different spatial scales ex situ (in piston cores and multi cores), and in situ (using a benthic lander equipped with a microelectrode profiler). Along a transect in the area 24 to 46° S and 165 to 117° W, cores from 10 out of 11 sites were oxygenated over their entire length (as much as 8 m below seafloor), with deep O2 concentrations \u3e150 μmol L−1. This represents the deepest oxygen penetration ever measured in marine sediments. High-resolution microprofiles from the surface sediment layer revealed a diffusive oxygen uptake between 0.1 and 1.3 mmol m−2 d−1, equal to a carbon mineralization rate of ~0.4–4.5 gC m−2 yr−1. This is in the lower range of previously reported fluxes for oligotrophic sediments but corresponds well to the low surface water primary production. Half of the pool of reactive organic matter was consumed in the top 1.5–6 mm of the sediment. Because of the inert nature of the deeper sediment, oxygen that is not consumed within the top centimeters diffuses downward to much greater depth. In deeper zones, a small O2 flux between 0.05 and 0.3 μmol m−2 d−1 was still present. This flux was nearly constant with depth, indicating extremely low O2 consumption rates. Modeling of the oxygen profiles suggests that the sediment is probably oxygenated down to the basalt, suggesting an oxygen flux from the sediment into the basaltic basement
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