Exploiting waste heat from combine harvesters to damage harvested weed seeds and reduce weed infestation

Weeds are mainly controlled with herbicides in intensive crop production, but this has resulted in increasing problems with herbicide-resistant weeds and public concerns about the unwanted side-effects of herbicide use. Therefore, there is a need for new alternative methods to reduce weed problems. One way to reduce weed infestation could be to collect or kill weed seeds produced in the growing season. Crop and weeds are harvested simultaneously with the combine harvester, but most of the weed seeds are returned with the chaff to the field creating new problems in future growing seasons. During the harvesting process, the harvester produces heat. Under normal harvest conditions, the exhaust gas temperature measured directly behind the turbocharger of the engine of a combine harvester may reach between 400 °C and 480 °C depending of the size of the engine. These high temperatures indicate that there is a potential for developing a system which perhaps could be utilized to kill or damage the weeds seeds. We investigate how much heat is needed to damage weed seeds significantly and focuses on the germination patterns over time in response to these treatments. We investigated if heat treatment of weed seeds could kill the seeds or reduce seed vigour or kill the seeds before they are returned to the field. The aim is to avoid harvested viable weed seeds being added to the soil seed bank. During the threshing and cleaning process in the combine harvester, most weed seeds and chaff are separated from the crop grains. After this separation, we imagine that the weed seeds could be exposed to a high temperature before they are returned to the field. Seeds of nine common weed species were treated with temperatures of 50 °C, 100 °C, 150 °C, 200 °C, and 250 °C for 0, 2, 5, 10, and 20 s, respectively. Afterwards, the seeds were germinated for fourteen days. Seeds were differently affected by the heat treatments. We found that 50 °C and 100 °C was insufficient to harm the seeds of all species significantly at all durations. Heating with a temperature of 50 °C and 100 °C showed a slight tendency to break the dormancy of Alopecurus myosuroides Huds. and Papaver rhoeas L., but the results were not statistically significant. Seeds treated with 150 °C gave varying results depending on the duration and the weed species. The germination of A. myosuroides was significantly repressed when seeds were exposed to 250 °C for 5 s. Most species were significantly damaged when they were exposed to 250 °C for more than 10 s. Our results showed that there is a potential to explore how the waste heat energy produced by combine harvesters can be exploited to either kill or reduce the vigour of weed seeds before they are returned to the field with the chaff

Extracellular vesicle proteomes reflect developmental phases of Bacillus subtilis

Extracellular vesicles (EV) are spherical membrane-bound vesicles with nano-scale diameters, which are shed to the extracellular region by most eukaryotic and prokaryotic cells. Bacterial EV are proposed to contribute to intercellular communication, bacterial survival and human pathogenesis as a novel secretion system. EV have been characterized from many Gram-negative species and, more recently, from several vegetative Gram-positive bacteria. Further characterization of EV and their molecular cargos will contribute to understanding bacterial physiology and to developing therapeutic approaches. Bacillus subtilis were observed to release EV to a similar extent during sporulation as during the vegetative growth phase. However, the two vesicular cargos show qualitatively and quantitatively different proteomes. Among 193 total proteins identified across both samples, 61 were shown to be significantly more abundant in EV shed by sporulating cells, with (log) ratio of spectral counts RSC > 1 and Fisher-exact test FDR < 5 %. Sixty-two proteins were found to be significantly more abundant in EV shed by vegetative cells. Membrane fusion was shown to take place between these EVs and Gram-positive cells. Biogenesis of EV is a continuous process over the entire life cycle of this sporulating bacterium. The formation of EV during sporulation is strongly supported by the delineation of protein content that differs from the proteome of EV formed by vegetative spores.https://doi.org/10.1186/s12014-016-9107-

Subcellular localization of MC4R with ADCY3 at neuronal primary cilia underlies a common pathway for genetic predisposition to obesity.

Most monogenic cases of obesity in humans have been linked to mutations in genes encoding members of the leptin-melanocortin pathway. Specifically, mutations in MC4R, the melanocortin-4 receptor gene, account for 3-5% of all severe obesity cases in humans1-3. Recently, ADCY3 (adenylyl cyclase 3) gene mutations have been implicated in obesity4,5. ADCY3 localizes to the primary cilia of neurons 6 , organelles that function as hubs for select signaling pathways. Mutations that disrupt the functions of primary cilia cause ciliopathies, rare recessive pleiotropic diseases in which obesity is a cardinal manifestation 7 . We demonstrate that MC4R colocalizes with ADCY3 at the primary cilia of a subset of hypothalamic neurons, that obesity-associated MC4R mutations impair ciliary localization and that inhibition of adenylyl cyclase signaling at the primary cilia of these neurons increases body weight. These data suggest that impaired signaling from the primary cilia of MC4R neurons is a common pathway underlying&nbsp;genetic causes of obesity in humans

Demonstration of vasoproliferative activity from mammalian retina

Vasoproliferative activity has been demonstrated in extracts of retinas from human, bovine, and feline sources. These retinal extracts are capable of stimulating (a) proliferation and thymidine uptake of bovine vascular endothelial cells in culture and (b) neovascularization on the chick chorioallantoic membrane. Extracts of skeletal muscle, cardiac muscle, and liver lack similar stimulatory activity. The activity is nondialyzable, stable at 56 degrees C, and inactivated at 100 degrees C. Retinal extracts stimulate the proliferation of corneal fibroblasts but have no effect on the proliferation of vascular smooth muscle cells. Indirect evidence suggests the liberation of a vasoproliferative factor from retina in several ocular disorders. The data in this report represent the first direct demonstration of vasoproliferative activity from mammalian retina