Research on Fatigue Damage and Affecting Factors of Defected Rock Mass Based on Ultrasonic Wave Velocity

In this paper the fatigue-loading test of defected rock-like specimen has been carried out by electro-hydraulic servo fatigue testing machine. At the same time, the ultrasonic data has been collected and analyzed by the digital ultrasonic instrument. The fatigue damage and influencing factors of the defective rock mass under dynamic load are studied. According to the experimental research and analysis, this paper chooses to define the damage variable by ultrasonic velocity, and the inverse function of the Logistic equation was used to describe the evolution curve of fatigue damage sample. The experimental data fitting results show that the damage model and the experimental data fit well. In addition, this paper analyzes the main influencing factors of fatigue damage in the test. The initial damage represents the damage state before the sample is cyclically loaded. Different initial damages have a significant effect on the fatigue life of the sample. In the case the initial damage is substantially the same, the upper limit stress is larger, the fatigue life of the sample is shorter, and conversely, the fatigue life is longer

MicroRNA-1 and Circulating Microvesicles Mediate the Protective Effects of Dantonic in Acute Myocardial Infarction Rat Models

Aim: To investigate the protective effect of dantonic in ischemic myocardial damage by evaluating the expression of circulating microvesicles (MVs) and microRNA-1 (miR-1) in two animal models.Methods: Two animal models of myocardial ischemia were established that were isoproterenol-induced myocardial ischemia (ISO-AMI) rat model and the acute myocardial infarction rat model induced by ligation of the left anterior descending coronary artery (LAD-AMI) of rat. To investigate the protective effect of dantonic, we observed the myocardial infarction size, creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) activities, cardiac troponin I (cTnI) level in serum, and the plasma levels of miR-1 and MVs.Results: The results showed that pretreatment with dantonic significantly attenuated the LAD-AMI induced myocardial damage by decreasing the size of myocardial infarction, CK, LDH, AST activities, and cTnI level in serum. High dose dantonic treatment could significantly abrogate the increased plasma levels of miR-1 and MVs as compared to the LAD rat model. In addition, pretreatment with dantonic also showed a significant myocardial protective effect through reducing the expression levels of CK, LDH, and AST as compared to the ISO-AMI model. Whereas the cTnI level was no significant difference between model group and control group, suggesting that the model caused less myocardial damage. In the ISO-induced myocardial ischemia model, there is no significant difference between the model group with the control group of MVs and miR-1 levels. This may be that miR-1 is reported as a biomarker of acute myocardial infarction. The pathological changes of IOS-induced acute myocardial ischemia model are also different from those of acute myocardial infarction.Conclusion: Dantonic showed the protective effect in these two ischemic myocardial injury rat models, whereas the circulating miR-1 and MVs levels were only ameliorated in the LAD rat model

Improving alignment accuracy on homopolymer regions for semiconductor-based sequencing technologies

BACKGROUND: Ion Torrent and Ion Proton are semiconductor-based sequencing technologies that feature rapid sequencing speed and low upfront and operating costs, thanks to the avoidance of modified nucleotides and optical measurements. Despite of these advantages, however, Ion semiconductor sequencing technologies suffer much reduced sequencing accuracy at the genomic loci with homopolymer repeats of the same nucleotide. Such limitation significantly reduces its efficiency for the biological applications aiming at accurately identifying various genetic variants. RESULTS: In this study, we propose a Bayesian inference-based method that takes the advantage of the signal distributions of the electrical voltages that are measured for all the homopolymers of a fixed length. By cross-referencing the length of homopolymers in the reference genome and the voltage signal distribution derived from the experiment, the proposed integrated model significantly improves the alignment accuracy around the homopolymer regions. CONCLUSIONS: Besides improving alignment accuracy on homopolymer regions for semiconductor-based sequencing technologies with the proposed model, similar strategies can also be used on other high-throughput sequencing technologies that share similar limitations

Mapping forests in monsoon Asia with ALOS PALSAR 50-m mosaic images and MODIS imagery in 2010.

Extensive forest changes have occurred in monsoon Asia, substantially affecting climate, carbon cycle and biodiversity. Accurate forest cover maps at fine spatial resolutions are required to qualify and quantify these effects. In this study, an algorithm was developed to map forests in 2010, with the use of structure and biomass information from the Advanced Land Observation System (ALOS) Phased Array L-band Synthetic Aperture Radar (PALSAR) mosaic dataset and the phenological information from MODerate Resolution Imaging Spectroradiometer (MOD13Q1 and MOD09A1) products. Our forest map (PALSARMOD50 m F/NF) was assessed through randomly selected ground truth samples from high spatial resolution images and had an overall accuracy of 95%. Total area of forests in monsoon Asia in 2010 was estimated to be ~6.3 × 10(6 )km(2). The distribution of evergreen and deciduous forests agreed reasonably well with the median Normalized Difference Vegetation Index (NDVI) in winter. PALSARMOD50 m F/NF map showed good spatial and areal agreements with selected forest maps generated by the Japan Aerospace Exploration Agency (JAXA F/NF), European Space Agency (ESA F/NF), Boston University (MCD12Q1 F/NF), Food and Agricultural Organization (FAO FRA), and University of Maryland (Landsat forests), but relatively large differences and uncertainties in tropical forests and evergreen and deciduous forests

Intensification of mesoscale convective systems in the East Asian rainband over the past two decades

As one of the major producers of extreme precipitation, mesoscale convective systems (MCSs) have received much attention. Recently, MCSs over several hotpots, including the Sahel and US Great Plains, have been found to intensify under global warming. However, relevant studies on the East Asian rainband, another MCS hotpot, are scarce. Here, by using a novel rain-cell tracking algorithm on a high spatiotemporal resolution satellite precipitation product, we show that both the frequency and intensity of MCSs over the East Asian rainband have increased by 21.8% and 9.8% respectively over the past two decades (2000–2021). The more frequent and intense MCSs contribute nearly three quarters to the total precipitation increase. The changes in MCSs are caused by more frequent favorable large-scale water vapor-rich environments that are likely to increase under global warming. The increased frequency and intensity of MCSs have profound impacts on the hydroclimate of East Asia, including producing extreme events such as severe flooding

Relationship between Neural Alteration and Perineural Invasion in Pancreatic Cancer Patients with Hyperglycemia

Background: Patients with higher levels of fasting serum glucose have higher death rates from pancreatic cancer compared to patients with lower levels of fasting serum glucose. However, the reasons have not been studied. The goal of the current study was to examine the neural alterations in pancreatic cancer patients with hyperglycemia and to identify the relationship between the neural alterations and perineural invasion. Methodology/Principal Findings: The clinical and pathological features of 61 formalin-fixed pancreatic cancer specimens and 10 normal pancreases as controls were analyzed. Furthermore, the expression of Protein Gene Product 9.5 (PGP9.5), Myelin P0 protein (MPP), NGF, TrkA, and p75 were examined by immunohistochemistry. The median number of nerves, the median area of neural tissue, and the median nerve diameter per 10 mm 2 were larger in the hyperglycemia group than those in the euglycemia group (p = 0.007, p = 0.009, and p = 0.004, respectively). The integrated optical density (IOD) of MPP staining was lower in the hyperglycemia group than those in the euglycemia group (p = 0.019), while the expression levels of NGF and p75 were higher in the hyperglycemia group than those in the euglycemia group (p = 0.002, and p = 0.026, respectively). The nerve bundle invasion of pancreatic cancer was more frequent in the hyperglycemia group than in the euglycemia group (p = 0.000). Conclusions/Significance: Nerve damage and regeneration occur simultaneously in the tumor microenvironment o

Cambogin Is Preferentially Cytotoxic to Cells Expressing PDGFR

Platelet-derived growth factor receptors (PDGFRs) have been implicated in a wide array of human malignancies, including medulloblastoma (MB), the most common brain tumor of childhood. Although significant progress in MB biology and therapeutics has been achieved during the past decades, MB remains a horrible challenge to the physicians and researchers. Therefore, novel inhibitors targeting PDGFR signaling pathway may offer great promise for the treatment of MB. In the present study, we investigated the cytotoxicity and mechanisms of cambogin in Daoy MB cells. Our results show that cambogin triggers significant S phase cell cycle arrest and apoptosis via down regulation of cyclin A and E, and activation of caspases. More importantly, further mechanistic studies demonstrated that cambogin inhibits PDGFR signaling in Daoy and genetically defined mouse embryo fibroblast (MEF) cell lines. These results suggest that cambogin is preferentially cytotoxic to cells expressing PDGFR. Our findings may provide a novel approach by targeting PDGFR signaling against MB

G protein-coupled receptor-mediated calcium signaling in astrocytes

Astrocytes express a large variety of G~protein-coupled receptors (GPCRs) which mediate the transduction of extracellular signals into intracellular calcium responses. This transduction is provided by a complex network of biochemical reactions which mobilizes a wealth of possible calcium-mobilizing second messenger molecules. Inositol 1,4,5-trisphosphate is probably the best known of these molecules whose enzymes for its production and degradation are nonetheless calcium-dependent. We present a biophysical modeling approach based on the assumption of Michaelis-Menten enzyme kinetics, to effectively describe GPCR-mediated astrocytic calcium signals. Our model is then used to study different mechanisms at play in stimulus encoding by shape and frequency of calcium oscillations in astrocytes.Comment: 35 pages, 6 figures, 1 table, 3 appendices (book chapter

Essays in empirical corporate finance : analyst and manager decisions

This dissertation consists of three essays in empirical corporate finance. In the first chapter, I examine whether CEOs imprint anchoring on corporate financial decisions. I develop a measure of “anchoring CEO” based on insider selling near 52-week high, and find that firms managed by anchoring CEOs are more likely to issue SEOs when stock prices are approaching 52-week highs. The effect is stronger for firms with higher valuation uncertainty. Anchoring CEOs also tend to conduct accelerated SEOs and raise less capital through these offerings near 52-week high. Overall, the results provide evidence that anchoring CEOs behave consistently across personal and professional domains. In the second chapter, I investigate whether 52-week high stock prices serve as reference points for analyst recommendation revisions. I find that analysts are more likely to downgrade stocks when the prices approach the 52-week high levels. This anchoring effect is stronger for stocks with higher information asymmetry but moderated by analysts’ reputation, work experience, and educational background. I also show that a strategy that takes long positions in stocks approaching 52-week high generates positive returns, and a strategy that shorts stocks with recommendation downgrades is less profitable for the downgrades near 52-week high than for other downgrades. Moreover, the downgraded firms with prices near 52-week high subsequently experience less negative earnings forecast revisions, compared with other downgraded firms. Collectively, the results indicate that downgrades near 52-week high are partially attributable to anchoring heuristic and less informative. In the third chapter, I study the cash holdings of innovative firms across countries. Using 8,379 innovative firms from 23 non-U.S. countries over 1990-2012, I find that firms with higher innovation efficiency (IE) hoard more cash. This IE-cash relation is more pronounced for firms in countries with less developed financial markets, better institutions and infrastructures for innovation, and more intense competition. The relation is also stronger for firms that are in industries with higher dependence on external financing and more technology-intensive, and financially constrained. These firms increase cash holdings by saving from cash flows and paying out less. Overall, this international evidence is consistent with the precautionary motive of cash holdings for innovative firms.published_or_final_versionEconomics and FinanceDoctoralDoctor of Philosoph