5 research outputs found
Determining crystal structures through crowdsourcing and coursework
We show here that computer game players can build high-quality crystal structures. Introduction of a new feature into the computer game Foldit allows players to build and real-space refine structures into electron density maps. To assess the usefulness of this feature, we held a crystallographic model-building competition between trained crystallographers, undergraduate students, Foldit players and automatic model-building algorithms. After removal of disordered residues, a team of Foldit players achieved the most accurate structure. Analysing the target protein of the competition, YPL067C, uncovered a new family of histidine triad proteins apparently involved in the prevention of amyloid toxicity. From this study, we conclude that crystallographers can utilize crowdsourcing to interpret electron density information and to produce structure solutions of the highest quality
Shuttling of PINK1 between Mitochondrial Microcompartments Resolved by Triple-Color Superresolution Microscopy
The
cytosolic <i>p</i>hosphatase and <i>ten</i>sin
homologue Pten-kinase PINK1 involved in mitochondrial quality
control undergoes a proteolytic process inside mitochondria. It has
been suggested that the protein is not fully imported into mitochondria
during this maturation. Here, we have established live cell triple-color
super-resolution microscopy by combining FPALM and tracking and localization
microscopy (TALM) in order to unravel the spatiotemporal organization
of the C-terminal kinase domain of PINK1 during this process. We find
that the kinase domain is imported into active mitochondria and colocalizes
with respiratory complex I at the inner mitochondrial membrane. When
the processing step inside mitochondria is inhibited or mitochondria
are de-energized, full length PINK1 distributes between the outer
and the inner mitochondrial membranes, indicating a holdup of import.
These findings give the molecular base for a dual role of PINK1î—¸inside
energized mitochondria and outside of de-energized mitochondria
Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization
Astrocytic chloride is brain state dependent and modulates inhibitory neurotransmission in mice
Astrocytes act as a dynamic Cl− reservoir regulating Cl− homeostasis in the CNS. Astrocytic Cl− is high and stable during sleep, it is lower during wakefulness and fluctuates in response to sensory input and motor activity. Efflux of Cl− from astrocytes supports inhibitory transmission in the CNS