Analisis Pengaruh Budaya Organisasi Dan Kompensasi Terhadap Kinerja Karyawan Dengan Motivasi Sebagai Variabel Intervening (Studi Kasus Pada PT. Lg Bagian Penjualan Indonesia Semarang)

The problems that occurred in the employee portion of sales LG Indonesia Semarang is adecline in performance is indicated by not achieving the target for 2015. The employeeperformance and motivation is also thought to be influenced by factors of organizationalculture and also compensation deemed not feasible by most employees. This study aimedto analyze the influence of organizational culture on the motivation and compensationand employee performance parts sales LG Indonesia Semarang. The population used inthis study were all employees of LG Indonesia Semarang. The sampling technique usedwas purposive sampling. Criteria samples taken were all employees of the salesdepartment LG Indonesia Semarang who have worked more than two years are 71nurses. The method of collecting the data in this study using questionnaires andinterviews. Methods of data analysis using path analysis. Based on the research,organizational culture and compensation have a positive effect on motivation andperformance, while motivation is also a positive effect on performance. Based on theresults Sobel Test to determine whether there is mediating the relationship between theindependent and dependent variables, it is known that motivation mediates the effect ofcompensation and organizational culture on performance

Kajian Pengelolaan Lahan Subdas Secang Kulonprogo YOGYAKARTA

Penelitian ini bertujuan untuk mengevaluasi kemampuan lahan, menyusun arahan penggunaanlahan dan mengkaji pengelolaan lahan SubDAS Secang. Metode yang digunakan dalam penelitianadalah sampel terpilih pada 48 satuan lahan. Penelitian menunjukkan bahwa kemampuan lahanSubDAS Secang terdiri atas kelas lahan I seluas 187 ha, kelas lahan II seluas 147 ha, kelas lahan IIIseluas 515,2 ha, kelas lahan IV seluas 1522,7 ha, kelas lahan V seluas 7,3 ha dan kelas lahan VI seluas1223,2 ha. Arahan penggunaan lahan SubDAS Secang berupa pertanian lahan basah seluas 326,85 ha,kawasan permukiman dan budidaya tanaman semusim seluas 200,55 ha, kawasan budidaya tanamanlahan kering seluas 525,81 ha, kawasan budidaya tanaman tahunan seluas 1981,31 ha, kawasanpenyangga seluas 716,54 ha. Pengelolaan lahan memberikan pedoman pemanfaatan lahan; daerah hilirsebagai daerah pemanfaatan untuk pertanian irigasi; daerah tengah diperuntukan permukiman danpembudidayaan tanaman lahan kering; serta daerah hulu sebagai daerah imbuhan diperuntukkanwanatani dan hutan penyangga

Additional file 1: of Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data

An Excel spreadsheet with three tabs listing 1) datasets generated in this study, 2) datasets used from the publically domain, together with key parameters, and 3) number of datasets used per analysis. (XLSX 21 kb

Additional file 2: of Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data

A PDF file with supplementary Tables S1–S3 and supplementary Figures S1–S10. (PDF 11655 kb

İki cinayette üç benzerlik

Taha Toros Arşivi, Dosya No: 161/A-Ahmet Taner KışlalıUnutma İstanbul projesi İstanbul Kalkınma Ajansı'nın 2016 yılı "Yenilikçi ve Yaratıcı İstanbul Mali Destek Programı" kapsamında desteklenmiştir. Proje No: TR10/16/YNY/010

Additional file 3: of Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data

An Excel spreadsheet with reference genome compositions. (XLSX 269 kb

Regional pairing brings chromosome territories close together.

<p>3D representation of stacks of confocal images from ES cells. Green: chromosome 7 painting, red: DNA FISH with a probe covering KvDMR near the distal end of chromosome 7, white: DNA FISH with a probe covering <i>Kcnn4</i> 25 Mb away from the proximal end of chromosome 7. FISH signals are mostly located in or close to the edge of their chromosome territory. Most nuclei display two separate chromosome 7 territories (<b>A</b>). When KvDMR signals are close together, the territory arrangements can be ‘touching’ (<b>B</b>) with the proximal ends pointing away from each other, ‘Y-shaped’ with a larger region aligning (<b>C</b>), or ‘aligned’ with most of the chromosome being parallel (<b>D</b>). These arrangements occur with very similar frequencies (n = 9, 8, 9, respectively).</p

4C-Seq reveals <i>trans</i>-allelic associations.

<p><b>A</b>) Example of 4C-Seq association profile surrounding the KvDMR bait in the middle of the <i>Kcnq1</i> gene (window size 1.5 Mb, sample B6xSD7, E13.5 liver). The first row shows the quantification of all non-duplicated 4C-Seq reads per 100 kb window for the maternal bait in <i>cis</i>. 4C-Seq reads can only occur at certain positions and each position was counted only once (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038983#pone.0038983.s010" target="_blank">File S2</a> for details). Colour and height of the bar reflect how many positions were found per 100 kb window (31 for the window that includes the bait). The second row shows associations of the maternal bait (B6) with the <i>trans</i> (paternal, SD7) allele. All reads identified as SD7 specific by ASAP were quantified per 100 kb window (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038983#pone.0038983.s010" target="_blank">File S2</a> for details), again counting every position only once. A scale bar indicates the location of the region on chromosome 7. Black bars below represent the location of genes with some labelled for orientation. The positions of 3C fragments classified as ‘stringently informative’ are depicted at the bottom (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038983#pone.0038983.s010" target="_blank">File S2</a> for details). The vast majority of associations occurs in <i>cis</i>. <i>Trans</i>-allelic associations occur most frequently with the homologous region. <b>B</b>) Overview over <i>cis</i> associations on chromosome 7 (sample B6xSD7, E13.5 liver, maternal bait, 100 kb windows, read positions counted only once, zoomed out from 1A). Height and colour of bars reflect how many read positions were found per 100 kb window. The bait is located near the telomeric end of chromosome 7. 4C-Seq reads are most frequent around the bait and drop away with increasing genomic distance. <b>C</b>) Summary of <i>trans</i>-allelic associations for E13.5 liver and placenta for different parental crosses. For each 200 kb window a certain number of 3C fragments are stringently informative for their allelic origin (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038983#pone.0038983.s010" target="_blank">File S2</a> for details). If a stringently informative fragment was found in a particular data set and identified as a <i>trans</i>-association with either the maternal or the paternal bait, it was counted as a hit. <i>Trans</i>-allelic associations per window are displayed as hits divided by the number of stringently informative fragments. If no bar is displayed, no <i>trans</i>-allelic associations were identified for this window. Below, locations of genes and distances from the KvDMR bait are indicated. For all samples, <i>trans</i>-allelic associations peak around the KvDMR region on the other allele. <b>D</b>) Comparison of homologous <i>trans</i>-allelic 4C-Seq associations with non-homologous <i>trans</i> associations in the genome. The genome was split into 1.4 Mb windows (matching the size of the chromosome 7 region carrying allelic information) and unique <i>trans</i> associations of the KvDMR bait with regions within these windows were counted. Data are shown as Tukey box-whisker plots. Data points marked by a red circle represent <i>trans</i>-allelic hits to the corresponding region on the other chromosome. m: maternal bait, p: paternal bait, BxS: cross B6xSD7, SxB: cross SD7xB6. Most regions do not associate with the KvDMR bait, however, some regions are over represented (outliers). <i>Trans</i>-allelic associations occur with a similar frequency to other non-homologous <i>trans</i> associations.</p

DNA FISH confirms high pairing frequency for the KvDMR region.

<p><b>A</b>) Examples of paired DNA FISH spots in E13.5 foetal liver. Red: probe covering the KvDMR region, green: probe covering the region around the myc gene, blue: DAPI counterstain. <b>B</b>) Pairing frequency of the KvDMR and myc regions. Each dot represents one biological sample, and for each sample 300 nuclei were counted in four technical replicates. Differences were assessed by unpaired t-test, ns: not significant, *: p<0.05, ***: p<0.001. KvDMR signals show higher pairing frequency than myc signals. <b>C</b>) Frequency distributions of radial distances of unpaired (black) and paired (blue) KvDMR DNA FISH signals in E13.5 liver (n = 93). Radial distances >1 can occur if the nucleus is not a perfect sphere. Radial distances are not different between paired and unpaired KvDMR FISH signals (t-test, p = 0.0668) indicating that pairing events can happen at all nuclear locations KvDMR alleles normally occupy. <b>D</b>) Distances between homologous alleles in E13.5 liver represented as Tukey box-whisker plots (n = 600). Interallelic distances were normalised to the radius of the nucleus (distance/radius). Differences were assessed by one-way ANOVA with Bonferroni’s multiple comparison post test. Simulated: a group of spots displaying the same radial distributions as KvDMR and myc FISH signals, respectively, were placed into a sphere at random and their interallelic distances determined (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038983#pone.0038983.s001" target="_blank">Fig. S1A</a>, B and Methods). While interallelic distances between myc signals show a distribution expected from their radial positions (no difference between sample and simulated, p>0.05), KvDMR signals are significantly closer together than expected (difference between sample and simulated, p<0.001). Also, KvDMR signals are generally closer together than myc signals. The same data is represented as a histogram in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038983#pone.0038983.s001" target="_blank">Fig. S1C</a> to illustrate the presence of a subpopulation of KvDMR signals that display very short interallelic distances.</p

Pairing is not limited to monoallelically expressed regions.

<p><b>A</b>) Extent of paired region on chromosome 7 in ES cells. Each dot represents the mean of three to four samples taken from different passages with the pairing frequency determined for each sample in four technical replicates of 300 nuclei. Whiskers represent standard deviation. Probes from the imprinted region on distal chromosome 7 are shown as open circles, probes from non-imprinted regions are shown as filled circles. Background shading indicates high pairing frequency (dark grey, above 3.5%), medium (light grey, 2.5–3.5%) and low pairing frequency (yellow, below 2.5%). The myc probe (chromosome 15) is displayed for comparison. Frequent pairing is observed for the distal end of chromosome 7 but not limited to the imprinted region. <b>B</b>) Pairing in imprinting deficient mutants. Each dot represents one biological sample with the pairing frequency determined in four technical replicates of 300 nuclei. The mean is represented by a line. Differences were assessed by unpaired t-test, ns: not significant. Wt: wildtype, KvDMR del: paternal deletion of KvDMR abolishing regional silencing and removing functionally important CTCF binding sites, RNA trunc: paternal truncation of the non-coding RNA Kcnq1ot1 responsible for monoallelic silencing, par ES: parthenogenetic ES cells harbouring two maternal genomes, andr ES: androgenetic ES cells harbouring two paternal genomes. Neither the monoallelic expression state nor the presence of a biparental genome is a prerequisite for frequent pairing in the region. <b>C</b>) Pairing of other imprinted and telomeric regions in ES cells. For a description of data points and background see A. Differences were assessed by unpaired t-test, ns: not significant, *: p<0.05. Imprinted regions are represented by open circles, regions close to the telomere by filled diamonds. Probes covering genes in various imprinted regions do not display high pairing frequency in contrast to probes located near telomeres.</p