Inflammatory and Glutamatergic Homeostasis Are Involved in Successful Aging

Whole body studies using long-lived growth hormone receptor gene disrupted or knock out (GHR-KO) mice report global GH resistance, increased insulin sensitivity, reduced insulin-like growth factor 1 (IGF-1), and cognitive retention in old-age, however, little is known about the neurobiological status of these mice. The aim of this study was to determine if glutamatergic and inflammatory markers that are altered in aging and/or age-related diseases and disorders, are preserved in mice that experience increased healthspan. We examined messenger ribonucleic acid (mRNA) expression levels in the brain of 4- to 6-, 8- to 10-, and 20- to 22-month GHR-KO and normal aging control mice. In the hippocampus, glutamate transporter 1 (GLT-1) and anti-inflammatory nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB)-p50 were elevated in 8- to 10-month GHR-KO mice compared with age-matched controls. In the hypothalamus, NFκB-p50, NFκB-p65, IGF-1 receptor (IGF-1R), glutamate/aspartate transporter (GLAST), and 2-amino-3-(5-methyl-3-oxo 2,3-dihydro-1,2 oxazol-4-yl) propanoic acid receptor subunit 1 (GluA1) were elevated in 8- to 10- and/or 20- to 22-month GHR-KO mice when comparing genotypes. Finally, interleukin 1-beta (IL-1β) mRNA was reduced in 4- to 6- and/or 8- to 10-month GHR-KO mice compared with normal littermates in all brain areas examined. These data support the importance of decreased brain inflammation in early adulthood and maintained homeostasis of the glutamatergic and inflammatory systems in extended longevity

Electron microscopy study of CeOx–Pd/α-Al2O3 catalysts for methane dry reforming

We have investigated the interaction between Pd and Ce in a 0.47 wt % CeOx–Pd 1 wt %/ -Al2O3 catalyst that is used in the reforming reaction of CH4 with CO2. The freshly reduced catalyst was characterized by various electron microscopy techniques, such as elemental mapping, Z-contrast imaging, and electron energy-loss spectroscopy to understand the role of Ce on a microscopic scale. The high spatial resolution elemental mapping indicates that CeOx is located in close proximity of the palladium nanoparticles. High-resolution lattice images and energy-loss spectra obtained in the vicinity of the Pd particles show an anisotropic distribution of CeOx crystallites limited to the interface region between Pd and the substrate but not covering the surface of the Pd nanoparticles. Energy-loss near edge fine structure of Pd M edges reveals that the Pd nanoparticles are not oxidized.Fil: Moreno, M. S.. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; ArgentinaFil: Wang, Feiya. National Institute for Nanotechnology; CanadáFil: Malac, M.. National Institute for Nanotechnology; CanadáFil: Kasama, T.. University of Cambridge; Reino UnidoFil: Gigola, Carlos Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Costilla, Ignacio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Sánchez, M. D.. Universidad Nacional del Sur. Departamento de Física; Argentin

A STUDY ON COPPER SURFACE PRE-TREATMENT AND WELDING WITH LASERS

ABSTRACT To improve the copper absorption coefficient to lasers, and to realize a high welding quality with a low power laser machine, a black-treating method was proposed in this study. With this method, the reflection coefficient of the copper to lasers with 1064nm wavelength could be reduced from 95% to 15.5%. The experimental study of copper welding with a 500 W fiber laser machine was carried out. The shape, the strength and the micro-hardness of the welding seam with different welding velocity and defocus amount were compared. And, the best welding parameter was obtained. Moreover, the laser welding quality of copper with black-treating and that with graphite coating was compared, and the result showed that the microhardness and the strength of the welding seam of copper with black-treating was better than that with graphite coating

Research on surface subsidence law in high-intensity mining of shallow buried with thick coal seam

Taking Daliuta Coal Mine in Western Shendong mining area as the engineering background, this paper selected typical shallow buried high-intensity mining panel 52307 to establish the observation station for surface movement. Combined RTK technology and 3D laser scanning technology for real-time monitoring, based on key layer theory and CISPM comprehensive surface subsidence prediction model software, the characteristics of surface movement and deformation, surface movement angular parameters and surface cracks in high-intensity mining of shallow buried was studied. The results showed that with the advance of the panel from the set-up room, the surface subsidence was small at the set-up room position and then increased suddenly and rapidly. Simultaneously, the subsidence curve became steep sharply. When the maximum subsidence value was reached, the subsidence velocity tended to slow down. The surface movement deformation was mainly concentrated in the middle of the panel, while the subsidence deformation around the panel and the influence range of surface movement was small. The surface movement angular parameters were quite larger in the Daliuta coal mine area, while the bedrock movement angle and boundary angle reached 87.7 ° and 84.1 ° respectively. The surface cracks caused by mining were in an overall “C” shape, mainly distributed in the middle of the panel. Then, As the working face continued to advance, the ground fissures continue to slowly extend, develop, and gradually close from the set-up room to the tailgate side, eventually forming a continuous surrounding "funnel" shape. Ground fissures always lagged behind the working face position and the lag distance of surface cracks increases linearly with the mining speed. According to the analysis of surface subsidence law and surface cracks development, it was concluded that under the condition of high-intensity mining of shallow buried with thick coal seam, due to the high mining intensity of the working face, fast advancing speed, single key layer structure and low occurrence horizon, the roof activity was intense and easy to slide and lose stability. As a result, it was appeared resulting in rapid convergence around the surface movement basin, serious damage in the middle, and intensive development of ground fissures

East Asian International Students and Psychological Well-Being: A Systematic Review

The present article reports a systematic review of the studies related to psychological well-being among East Asian international students. A total of 18 quantitative studies published in peer-reviewed journals from 2000 to 2011 were reviewed. Our review revealed three major results: (1) a majority of researchers (n=13, 72.2%) tend to choose Chinese international students as a representative of East Asian and Asian international students in their studies; (2) studies on psychological well-being of East Asian international students are closely associated with the following variables: length of stay in host country, English proficiency, attitudes toward seeking help, depression, and acculturation; (3) depression was the most frequently reported variable (n=6,33.3%), followed by acculturation (n=5, 27.8%). Recommendations for further research in psychological well-being were provided

Analysis of the Role of General Practitioners Services in Rural Areas during the COVID-19 Epidemic

Since the outbreak of the COVID-19, various regions of China have been rapidly deployed under the leadership of the Central Committee of the Party to actively prevent and control the COVID-19. The rural areas of my country have weak links to the prevention and management of public health emergencies. Problems include lack of medical and health resources and farmers’ low awareness of epidemic prevention. Situations that correspond to the prevention and management of the COVID-19 are more serious. As the patient’s first contact and “gatekeeper” in the fight against the epidemic, the general practitioner is responsible for the “first visit-subsequent ongoing intervention”. This article is about the prevention and control of the COVID-19 epidemics and epidemic prevention in terms of dissemination of knowledge, informed crowd control, joint prevention and control, and standardized management of people. This is a summary of the efforts of general practitioners. Quarantine at home, interactive referrals to medical consortiums, special care for contracted families. The function during the management period aims to analyze the role played by general practitioners during the epidemic and to provide new ideas for the prevention and management of the epidemic. Provide more targeted general practitioner-style services in rural areas to promote the implementation and improvement of health and poverty alleviation. The health level of the rural population provides a theoretical standard

Duration of Rapamycin Treatment Has Differential Effects on Metabolism in Mice

SummaryThe evolutionarily conserved target of rapamycin (TOR) signaling controls growth, metabolism, and aging. In the first robust demonstration of pharmacologically-induced life extension in mammals, longevity was extended in mice treated with rapamycin, an inhibitor of mechanistic TOR (mTOR). However, detrimental metabolic effects of rapamycin treatment were also reported, presenting a paradox of improved survival despite metabolic impairment. How rapamycin extended lifespan in mice with such paradoxical effects was unclear. Here we show that detrimental effects of rapamycin treatment were only observed during the early stages of treatment. These effects were reversed or diminished in mice treated for 20 weeks, with better metabolic profiles, increased oxygen consumption and ketogenesis, and markedly enhanced insulin sensitivity. Thus, prolonged rapamycin treatment lead to beneficial metabolic alterations, consistent with life extension previously observed. Our findings provide a likely explanation of the “rapamycin paradox” and support the potential causal importance of these metabolic alterations in longevity

Temporal bone osteoblastoma involving temporomandibular joint diagnosed as simple disc disorders: A case report

BackgroundOsteoblastoma is quite rare in the oromaxillo-facial region, while the mandible is always the predilection. However, in our case, the lesion was located in the left temporal articular tubercle, involving the adjacent skull base, which is extremely rare in the literature.Case reportsIt had been diagnosed as the most common temporomandibular joint disorder in the local hospital before the patient came to our department, mainly due to the primary symptom, that was, the patient got pain in the left temporomandibular joint area while opening the mouth. However, we found a mass of bone lesions at the left temporal articular tubercle in MRI and cone beam CT, and it turned out to be an osteoblastoma after surgery. The patient's primary symptom disappeared after recovering from the surgery, and there have been no indications of complication or recurrence up to now.ConclusionOsteoblastoma is very rare in the temporomandibular joint region. It could easily miss the possibility of a benign tumor due to its unusual location and confusing chief complaint in this case. Our report provides experience in the identification of osteoblastoma in rare sites

Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity

Individuals homozygous for the “Z” mutation in alpha-1 antitrypsin deficiency are known to be at increased risk for liver disease. It has also become clear that some degree of risk is similarly conferred by the heterozygous state. A lack of model systems that recapitulate heterozygosity in human hepatocytes has limited the ability to study the impact of a single Z alpha-1 antitrypsin (ZAAT) allele on hepatocyte biology. Here, we describe the derivation of syngeneic induced pluripotent stem cells (iPSCs) engineered to determine the effects of ZAAT heterozygosity in iPSC-hepatocytes (iHeps). We find that heterozygous MZ iHeps exhibit an intermediate disease phenotype and share with ZZ iHeps alterations in AAT protein processing and downstream perturbations including altered endoplasmic reticulum (ER) and mitochondrial morphology, reduced mitochondrial respiration, and branch-specific activation of the unfolded protein response in cell subpopulations. Our model of MZ heterozygosity thus provides evidence that a single Z allele is sufficient to disrupt hepatocyte homeostatic function.This work was supported by an Alpha-1 Foundation John W. Walsh Translational Research Award (to J.E.K.); a CJ Martin Early Career Fellowship from the Australian National Health and Medical Research Council (to R.B.W.); NIH grant R01HL095993 (to D.N.K.); and NIH grants R01DK101501 (to A.A.W.) and R01DK117940 (to A.N.H. and A.A.W.). iPSC distribution and disease modeling is supported by NIH grants U01TR001810 (to D.N.K. and A.A.W.) and N0175N92020C00005 (to D.N.K.); and by The Alpha-1 Project (TAP), a wholly owned subsidiary of the Alpha-1 Foundation (to D.N.K. and A.A.W.)

Metabolic effects of intra-abdominal fat in GHRKO mice

Mice with targeted deletion of the growth hormone receptor (GHRKO mice) are growth hormone (GH) resistant, small, obese, hypoinsulinemic, highly insulin sensitive and remarkably long-lived. To elucidate the unexpected coexistence of adiposity with improved insulin sensitivity and extended longevity, we examined effects of surgical removal of visceral (epididymal and perinephric) fat on metabolic traits related to insulin signaling and longevity. Comparison of results obtained in GHRKO mice and in normal animals from the same strain revealed disparate effects of visceral fat removal (VFR) on insulin and glucose tolerance, adiponectin levels, accumulation of ectopic fat, phosphorylation of insulin signaling intermediates, body temperature, and respiratory quotient (RQ). Overall, VFR produced the expected improvements in insulin sensitivity and reduced body temperature and RQ in normal mice and had opposite effects in GHRKO mice. Some of the examined parameters were altered by VFR in opposite directions in GHRKO and normal mice, and others were affected in only one genotype or exhibited significant genotype x treatment interactions. Functional differences between visceral fat of GHRKO and normal mice were confirmed by measurements of adipokine secretion, lipolysis, and expression of genes related to fat metabolism. We conclude that in the absence of GH signaling, the secretory activity of visceral fat is profoundly altered and unexpectedly promotes enhanced insulin sensitivity. The apparent beneficial effects of visceral fat in GHRKO mice may also explain why reducing adiposity by calorie restriction fails to improve insulin signaling or further extend longevity in these animals