Effect of Larval Nutrition on Maternal mRNA Contribution to the Drosophila Egg.

Embryonic development begins under the control of maternal gene products, mRNAs and proteins that the mother deposits into the egg; the zygotic genome is activated some time later. Maternal control of early development is conserved across metazoans. Gene products contributed by mothers are critical to many early developmental processes, and set up trajectories for the rest of development. Maternal deposition of these factors is an often-overlooked aspect of parental investment. If the mother experiences challenging environmental conditions, such as poor nutrition, previous studies in Drosophila melanogaster have demonstrated a plastic response wherein these mothers may produce larger eggs to buffer the offspring against the same difficult environment. This additional investment can produce offspring that are more fit in the challenging environment. With this study, we ask whether D. melanogaster mothers who experience poor nutrition during their own development change their gene product contribution to the egg. We perform mRNA-Seq on eggs at a stage where all mRNAs are maternally derived, from mothers with different degrees of nutritional limitation. We find that nutritional limitation produces similar transcript changes at all degrees of limitation tested. Genes that have lower transcript abundance in nutritionally limited mothers are those involved in translation, which is likely one of the most energetically costly processes occurring in the early embryo. We find an increase in transcripts for transport and localization of macromolecules, and for the electron transport chain. The eggs produced by nutrition-limited mothers show a plastic response in mRNA deposition, which may better prepare the future embryo for development in a nutrition-limited environment

The Dynamical State fo the Starless Dense Core FeSt 1-457: A Pulsating Globule?

High resolution molecular line observations of CS, HCO+, C18O and N2H+ were obtained toward the starless globule FeSt 1-457 in order to investigate its kinematics and chemistry. The HCO+ and CS spectra show clear self-reversed and asymmetric profiles across the face of the globule. The sense of the observed asymmetry is indicative of the global presence of expansion motions in the outer layers of the globule. These motions appear to be subsonic and significantly below the escape velocity of the globule. Comparison of our observations with near-infrared extinction data indicate that the globule is gravitationally bound. Taken together these considerations lead us to suggest that the observed expansion has its origin in an oscillatory motion of the outer layers of the globule which itself is likely in a quasi-stable state near hydrostatic equilibrium. Analysis of the observed linewidths of CO and N2H+ confirm that thermal pressure is the dominant component of the cloud's internal support. A simple calculation suggests that the dominant mode of pulsation would be an l = 2 mode with a period of 0.3 Myr. Deformation of the globule due to the large amplitude l = 2 oscillation may be responsible for the double-peaked structure of the core detected in high resolution extinction maps. Detailed comparison of the molecular-line observations and extinction data provides evidence for significant depletion of C18O and perhaps HCO+ while N2H+ may be undepleted to a cloud depth of about 40 magnitudes of visual extinction.Comment: to appear in ApJ vol 665 20 August 2007

Current Star Formation in the Ophiuchus and Perseus Molecular Clouds: Constraints and Comparisons from Unbiased Submillimeter and Mid-Infrared Surveys. II

We present a census of the population of deeply embedded young stellar objects (YSOs) in the Ophiuchus molecular cloud complex based on a combination of Spitzer Space Telescope mid-infrared data from the "Cores to Disks" (c2d) legacy team and JCMT/SCUBA submillimeter maps from the COMPLETE team. We have applied a method developed for identifying embedded protostars in Perseus to these datasets and in this way construct a relatively unbiased sample of 27 candidate embedded protostars with envelopes more massive than our sensitivity limit (about 0.1 M_sun). Embedded YSOs are found in 35% of the SCUBA cores - less than in Perseus (58%). On the other hand the mid-infrared sources in Ophiuchus have less red mid-infrared colors, possibly indicating that they are less embedded. We apply a nearest neighbor surface density algorithm to define the substructure in each of the clouds and calculate characteristic numbers for each subregion - including masses, star formation efficiencies, fraction of embedded sources etc. Generally the main clusters in Ophiuchus and Perseus (L1688, NGC1333 and IC348) are found to have higher star formation efficiencies than small groups such as B1, L1455 and L1448, which on the other hand are completely dominated by deeply embedded protostars. We discuss possible explanations for the differences between the regions in Perseus and Ophiuchus, such as different evolutionary timescales for the YSOs or differences, e.g., in the accretion in the two clouds.Comment: Accepted for publication in ApJ (56 pages, 13 figures; abstract abridged). Version with full-resolution figures available at http://www.astro.uni-bonn.de/~jes/paper120.pd

A Turbulent Origin for Flocculent Spiral Structure in Galaxies

The flocculent structure of star formation in 7 galaxies has a Fourier transform power spectrum for azimuthal intensity scans with a power law slope that increases systematically from -1 at large scales to -1.7 at small scales. This is the same pattern as in the power spectra for azimuthal scans of HI emission in the Large Magellanic Clouds and for flocculent dust clouds in galactic nuclei. The steep part also corresponds to the slope of -3 for two-dimensional power spectra that have been observed in atomic and molecular gas surveys of the Milky Way and the Large and Small Magellanic Clouds. The same power law structure for star formation arises in both flocculent and grand design galaxies, which implies that the star formation process is the same in each. Fractal Brownian motion models that include discrete stars and an underlying continuum of starlight match the observations if all of the emission is organized into a global fractal pattern with an intrinsic 1D power spectrum having a slope between 1.3 and 1.8. We suggest that the power spectrum of optical light in galaxies is the result of turbulence, and that large-scale turbulent motions are generated by sheared gravitational instabilities which make flocculent spiral arms first and then cascade to form clouds and clusters on smaller scales.Comment: accepted for ApJ, 31 pg, 9 figure

Hubble Space Telescope Photometry of the Dwarf Spheroidal Galaxy ESO 410-G005

We present HST WFPC2 imaging of the nearby low-surface-brightness dwarf spheroidal galaxy ESO 410-G005, which has been resolved into stars for the first time. The resulting color-magnitude diagram for about 2500 stars shows a red giant branch branch with a tip at I=(22.4+-0.15), which yields a distance of D=(1.9+-0.2) Mpc. ESO 410-G005 is found to be metal-poor with a mean metallicity of (-1.8+-0.4) dex estimated from its red giant branch. Upper asymptotic giant branch stars appear to be present near the center of the galaxy, indicative of a substantial, centrally concentrated intermediate-age population, unless these objects are artifacts of crowding. Previous studies did not detect ESO 410-G005 in H alpha or in HI. ESO 410-G005 is a probable member of the Sculptor group. Its linear separation from the nearest spiral, NGC 55, is 230 kpc on the sky. The deprojected separation ranges from 340 to 615 kpc depending on the assumed distance of NGC 55. ESO 410 G005 appears to be a relatively isolated dSph within the Sculptor group. Its absolute magnitude, Mv = (-12.1+-0.2) mag, its central surface brightness, mu_V = (22.7+-0.1) mag/arcsec^2, and its mean metallicity, [Fe/H] = (-1.8+-0.4) dex, follow the trend observed for dwarf galaxies in the Local Group. (abridged)Comment: Accepted for publication in the Astrophysical Journal, Vol. 542 (Oct 20). 23 pages in AASTEX style, 9 figures, partially in gif format to save spac

Magellanic Cloud Periphery Carbon Stars IV: The SMC

The kinematics of 150 carbon stars observed at moderate dispersion on the periphery of the Small Magellanic Cloud are compared with the motions of neutral hydrogen and early type stars in the Inter-Cloud region. The distribution of radial velocities implies a configuration of these stars as a sheet inclined at 73+/-4 degrees to the plane of the sky. The near side, to the South, is dominated by a stellar component; to the North, the far side contains fewer carbon stars, and is dominated by the neutral gas. The upper velocity envelope of the stars is closely the same as that of the gas. This configuration is shown to be consistent with the known extension of the SMC along the line of sight, and is attributed to a tidally induced disruption of the SMC that originated in a close encounter with the LMC some 0.3 to 0.4 Gyr ago. The dearth of gas on the near side of the sheet is attributed to ablation processes akin to those inferred by Weiner & Williams (1996) to collisional excitation of the leading edges of Magellanic Stream clouds. Comparison with pre LMC/SMC encounter kinematic data of Hardy, Suntzeff, & Azzopardi (1989) of carbon stars, with data of stars formed after the encounter, of Maurice et al. (1989), and Mathewson et al. (a986, 1988) leaves little doubt that forces other than gravity play a role in the dynamics of the H I.Comment: 30 pages; 7 figures, latex compiled, 1 table; to appear in AJ (June 2000

Low-extinction windows in the inner Galactic Bulge

We built K band extinction maps in the area of two candidate low-extinction windows in the inner Bulge: W0.2-2.1 at (l,b) = (0.25o,-2.15o), and W359.4-3.1 at (l,b) = (359.40o,-3.10o). We employed JHKs photometry from the 2MASS Point Source Catalog. Extinction values were determined by fitting the upper giant branch found in the present 2MASS Ks x J-Ks diagrams to a de-reddened bulge stellar population reference giant branch. We tested the method on the well known Baade's and Sgr I windows: the 2MASS mean extinction values in these fields agreed well with those of previous studies. We confirm the existence of low-extinction windows in the regions studied, as local minima in the A_K maps reaching A_K values about 2 standard deviations below the mean values found in the neighbouring areas. Schlegel et al.'s (1998) FIR extinction maps, which integrate dust contributions throughout the Galaxy, are structurally similar to those derived with 2MASS photometry in the two studied windows. We thus conclude that the dust clouds affecting the 2MASS and FIR maps in these directions are basically the same and are located on foreground of the bulk of bulge stars. However, the A_K absolute values differ significantly. In particular, the FIR extinction values for W359.4-3.1 are a factor ~1.45 larger than those derived from the 2MASS photometry. Possible explanations of this effect are discussed. The lower Galactic latitudes of the low-extinction windows W359.4-3.1 and W0.2-2.1, as compared to Baade's Window, make them promising targets for detailed studies of more central bulge regions.Comment: 8 pages, 10 figures, LaTeX, aa.cls. To appear in Astron. & Astroph., in pres

Detection of a Corrugated Velocity Pattern in the Spiral Galaxy NGC 5427

Here we report the detection, in Halpha emission, of a radial corrugation in the velocity field of the spiral galaxy NGC 5427. The central velocity of the Halpha line displays coherent, wavy-like variations in the vicinity of the spiral arms. The spectra along three different arm segments show that the maximum amplitude of the sinusoidal line variations are displaced some 500 pc from the central part of the spiral arms. The peak blueshifted velocities appear some 500 pc upstream the arm, whereas the peak redshifted velocities are located some 500 pc downstream the arm. This kinematical behavior is similar to the one expected in a galactic bore generated by the interaction of a spiral density wave with a thick gaseous disk, as recently modeled by Martos & Cox (1998).Comment: Accepted for publication in Ap

Magellanic Cloud Structure from Near-IR Surveys II: Star Count Maps and the Intrinsic Elongation of the LMC

I construct a near-IR star count map of the LMC and demonstrate, using the viewing angles derived in Paper I, that the LMC is intrinsically elongated. I argue that this is due to the tidal force from the Milky Way. The near-IR data from the 2MASS and DENIS surveys are used to create a star count map of RGB and AGB stars, which is interpreted through ellipse fitting. The radial number density profile is approximately exponential with a scale-length 1.3-1.5 kpc. However, there is an excess density at large radii that may be due to the tidal effect of the Milky Way. The position angle and ellipticity profile converge to PA_maj = 189.3 +/- 1.4 degrees and epsilon = 0.199 +/- 0.008 for r > 5 deg. At large radii there is a drift of the center of the star count contours towards the near side of the plane, which can be undrestood as due to viewing perspective. The fact that PA_maj differes from the line of nodes position angle Theta = 122.5 +/- 8.3 (cf. Paper I) indicates that the LMC disk is not circular, but has an intrinsic ellipticity of 0.31. The LMC is elongated in the general direction of the Galactic center, and is elongated perpendicular to the Magellanic Stream and the velocity vector of the LMC center of mass. This suggests that the elongation of the LMC has been induced by the tidal force of the Milky Way. The position angle of the line of nodes differs from the position angle Theta_max of the line of maximum line of sight velocity gradient: Theta_max - Theta = 20-60 degrees. This could be due to: (a) streaming along non-circular orbits in the elongated disk; (b) uncertainties in the transverse motion of the LMC center of mass; (c) precession and nutation of the LMC disk as it orbits the Milky Way (expected on theoretical grounds). [Abridged]Comment: Astronomical Journal, in press. 34 pages, LaTeX, with 7 PostScript figures. Contains minor revisions with respect to previously posted version. Check out http://www.stsci.edu/~marel/lmc.html for a large scale (23x21 degree) stellar number-density image of the LMC constructed from RGB and AGB stars in the 2MASS and DENIS surveys. The paper is available with higher resolution color figures from http://www.stsci.edu/~marel/abstracts/abs_R32.htm

Neutral Hydrogen and Star Formation in the Irregular Galaxy NGC 2366

We present UBVJHKHalpha and HI data of the irregular galaxy NGC 2366. It is a normal boxy-shaped disk seen at high inclination angle. We do not see any unambiguous observational signature of a bar. There is an asymmetrical extension of stars along one end of the major axis of the galaxy, and this is where the furthest star-forming regions are found, at 1.3R_Holmberg. The HI is normal in many respects but shows some anomalies: 1) The integrated HI shows two ridges running parallel to the major axis that deproject to a large ring. 2) The velocity field exhibits several large-scale anomalies superposed on a rotating disk. 3) The inclination and position angles derived from the kinematics differ from those dervied from the optical and HI mor- phology. 4) There are regions in the HI of unusually high velocity dispersion that correlate with deficits of HI emission in a manner suggestive of long-range, turbulent pressure equilibrium. Star-forming regions are found where the gas densities locally exceed 6 Msolar/pc^2. NGC 2366, like other irregulars, has low gas densities relative to the critical gas densities of gravitational instability models. Because of the lack of shear in the optical galaxy, there is little competition to the slow gravitational contraction that follows energy dissipation. However, the peak gas densities in the star-forming regions are equal to the local tidal densities for gravitational self-binding of a rotating cloud. Evidently the large scale gas concentrations are marginally bound against background galactic tidal forces. This condition for self-binding may be more fundamental than the instability condition because it is local, three-dimensional, and does not involve spiral arm generation as an intermediate step toward star formation.Comment: To be published in ApJ; better figures available ftp.lowell.edu, cd pub/dah/n2366pape