Redetermination of Fe2[BP3O12]

Explorations of phases in the quaternary FeIII–BIII–PV–O system prepared by the high temperature solution growth (HTSG) method led to single-crystal growth of anhydrous diiron(III) borotriphosphate, Fe2[BP3O12]. This phase has been synthesized previously as a microcrystalline material and its structure refined in space group P3 from powder X-ray diffraction data using the Rietveld method [Chen et al. (2004 ▶). J. Inorg. Mater. 19, 429-432]. In the current single-crystal study, it was shown that the correct space group is P63/m. The three-dimensional structure of the title compound is built up from FeO6 octa­hedra (3.. symmetry), trigonal–planar BO3 groups ( symmetry) and PO4 tetra­hedra (m.. symmetry). Two FeO6 octa­hedra form Fe2O9 dimers via face-sharing, while the anionic BO3 and PO4 groups are connected via corner-sharing to build up the [BP3O12]6− anion. Both units are inter­connected via corner-sharing

Regio- and Stereoselective Synthesis of Tetrasubstituted Alkenes via Ruthenium(II)-Catalyzed C–H Alkenylation/Directing Group Migration

Herein we report the regio- and stereoselective synthesis of tetrasubstituted alkenes from N-carbamoyl indoles and alkynes via ruthenium(II)-catalyzed C−H alkenylation/directing group migration, in which the carbamoyl directing group is endowed with a dual role of auxiliary group and migrating acylation reagent via C−N bond cleavage. This method features broad substrate scope, high atom- and step-economy, good functional group tolerance and mild redox-neutral conditions at room temperature. A series of structurally diverse tetrasubstituted alkenes were synthesized in good to excellent yields, highlighting the efficiency of this method

Theoretical and Numerical Analysis of 1 : 1 Main Parametric Resonance of Stayed Cable Considering Cable-Beam Coupling

For the 1 : 1 main parametric resonances problems of cable-bridge coupling vibration, a main parametric resonances model considering cable-beam coupling is developed and dimensionless parametric resonances differential equations are derived. The main parametric resonances characteristics are discussed by means of multiscale approximation solution methods. Using an actual cable of cable-stayed bridge project for research object, numerical simulation analysis under a variety of conditions is illustrated. The results show that when the coupling system causes 1 : 1 parametric resonance, nonlinear main parametric resonances in response are unrelated to initial displacement of the cable, but with the increase of deck beam end vertical initial displacement increases, accompanied with a considerable “beat” vibration. When the vertical initial displacement of deck beam end is 10−6 m order of magnitude or even smaller, “beat” vibration phenomenon of cable and beam appears. Displacement amplitude of the cable is small and considerable amplitude vibration may not occur at this time, only making a slight stable “beat” vibration in the vicinity of the equilibrium position, which is different from 2 : 1 parametric resonance condition of cable-bridge coupling system. Therefore, it is necessary to limit the initial displacement excitation amplitude of beam end and prevent the occurrence of amplitude main parametric excitation resonances

Awareness research report of hematological disease gene detection in the clinical application

目的  探讨医务工作者对血液病基因检测临床应用的认知程度。方法  对参加“第三届陆道培血液病高峰论坛”的371名学者进行了以微信为平台的调查研究，调查内容涉及被调查者的基本信息、工作习惯以及他们对基因检测临床应用多方面的观点。结果  调查对象的年龄主要分布在40岁以下，基因检测在三甲医院或高水平专科医院内应用的频度较高，有32.36% 被调查者认为费用过高是基因检测临床应用最主要的制约因素，68.2% 调查对象可以容忍报告在5-10个工作日内出具，对于基因突变报告有49.1% 临床医生认为报告结果太复杂。结论  基因检测已经在临床诊断方面得到较为广泛的应用，尤其是三甲医院和高水平专科医院，费用、有明确临床意义的临床检测项目较少及时间是基因检测临床应用的主要制约因素，大部分临床医生希望基因检测报告可以在5-10个工作日内出具，并且认为报告结果太复杂。Objective: To investigate the cognition of the medical workers on hematological disease gene detection in the clinical application.  Methods: We investigated 371 scholars participating in the “Third Ludaopei Hematology Forum” through WeChat. Investigation contents include the basic information of respondents, work habits and their views on gene testing in clinical application. Results: The age of the respondents are mainly less than 40 years old. Genetic testing finds wide application in the tertiary hospitals and high level of specialized hospitals. 32.36% of respondents believe that genetic testing cost is the main factor restricting the clinical application; 68.2% respondents can tolerate the fact that report is issued at 5-10 working days. For gene mutations report, 49.1% clinical doctors think the results are too complicated. Conclusion: Genetic testing in clinical diagnosis has been more widely applied, especially for the tertiary hospitals and high level of specialized hospitals. The cost, time and the fewness of clinical testing programs which are clinically significant, are the main factors which restricted the clinical application of genetic testing. Most clinicians hope that genetic testing report will be issued at 5-10 working days, and deem that the results are too complicated.

Milestones in Autonomous Driving and Intelligent Vehicles Part II: Perception and Planning

Growing interest in autonomous driving (AD) and intelligent vehicles (IVs) is fueled by their promise for enhanced safety, efficiency, and economic benefits. While previous surveys have captured progress in this field, a comprehensive and forward-looking summary is needed. Our work fills this gap through three distinct articles. The first part, a "Survey of Surveys" (SoS), outlines the history, surveys, ethics, and future directions of AD and IV technologies. The second part, "Milestones in Autonomous Driving and Intelligent Vehicles Part I: Control, Computing System Design, Communication, HD Map, Testing, and Human Behaviors" delves into the development of control, computing system, communication, HD map, testing, and human behaviors in IVs. This part, the third part, reviews perception and planning in the context of IVs. Aiming to provide a comprehensive overview of the latest advancements in AD and IVs, this work caters to both newcomers and seasoned researchers. By integrating the SoS and Part I, we offer unique insights and strive to serve as a bridge between past achievements and future possibilities in this dynamic field.Comment: 17pages, 6figures. IEEE Transactions on Systems, Man, and Cybernetics: System

Loss of circSRY reduces γH2AX level in germ cells and impairs mouse spermatogenesis.

Sry on the Y chromosome is the master switch of sex determination in mammals. It has been well established that Sry encodes a transcription factor that is transiently expressed in somatic cells of the male gonad, leading to the formation of testes. In the testis of adult mice, Sry is expressed as a circular RNA (circRNA) transcript. However, the physiological function of Sry circRNA (circSRY) remains unknown since its discovery in 1993. Here we show that circSRY is mainly expressed in the spermatocytes, but not in mature sperm or somatic cells of the testis. Loss of circSRY led to germ cell apoptosis and the reduction of sperm count in the epididymis. The level of γH2AX was decreased, and failure of XY body formation was noted in circSRY KO germ cells. Further study demonstrated that circSRY directly bound to miR-138-5p in spermatocytes, and in vitro assay suggested that circSRY regulates H2AX mRNA through sponging miR-138-5p. Our study demonstrates that, besides determining sex, Sry also plays an important role in spermatogenesis as a circRNA

High remission and low relapse with prolonged intensive DMARD therapy in rheumatoid arthritis (PRINT): A multicenter randomized clinical trial

Objectives: To determine whether prolonged intensive disease-modifying antirheumatic drug (DMARD) treatment (PRINT) leads to high remission and low relapse rates in patients with severe rheumatoid arthritis (RA). Methods: In this multicenter, randomized and parallel treatment trial, 346 patients with active RA (disease activity score (28 joints) [DAS28] (erythrocyte sedimentation rate [ESR]) > 5.1) were enrolled from 9 centers. In phase 1, patients received intensive treatment with methotrexate, leflunomide, and hydroxychloroquine, up to 36 weeks, until remission (DAS28 ≤ 2.6) or a low disease activity (2.6 < DAS28 ≤ 3.2) was achieved. In phase 2, patients achieving remission or low disease activity were followed up with randomization to 1 of 2 step-down protocols: leflunomide plus hydroxychloroquine combination or leflunomide monotherapy. The primary endpoints were good European League Against Rheumatism (EULAR) response (DAS28 (ESR) < 3.2 and a decrease of DAS28 by at least 1.2) during the intensive treatment and the disease state retention rate during step-down maintenance treatment. Predictors of a good EULAR response in the intensive treatment period and disease flare in the maintenance period were sought. Results: A good EULAR response was achieved in 18.7%, 36.9%, and 54.1% of patients at 12, 24, and 36 weeks, respectively. By 36 weeks, 75.4% of patients achieved good and moderate EULAR responses. Compared with those achieving low disease activity and a high health assessment questionnaire (HAQ > 0.5), patients achieving remission (DAS28 ≤ 2.6) and low HAQ (≤ 0.5) had a significantly higher retention rate when tapering the DMARDs treatment (P = 0.046 and P = 0.01, respectively). There was no advantage on tapering to combination rather than monotherapy. Conclusions: Remission was achieved in a proportion of patients with RA receiving prolonged intensive DMARD therapy. Low disease activity at the start of disease taper leads to less subsequent flares. Leflunomide is a good maintenance treatment as single treatment

Glucose and sucrose signaling modules regulate the arabidopsis juvenile-to-adult phase transition

Summary: CINV1, converting sucrose into glucose and fructose, is a key entry of carbon into cellular metabolism, and HXK1 functions as a pivotal sensor for glucose. Exogenous sugars trigger the Arabidopsis juvenile-to-adult phase transition via a miR156A/SPL module. However, the endogenous factors that regulate this process remain unclear. In this study, we show that sucrose specifically induced the PAP1 transcription factor directly and positively controls CINV1 activity. Furthermore, we identify a glucose feed-forward loop (sucrose-CINV1-glucose-HXK1-miR156-SPL9-PAP1-CINV1-glucose) that controls CINV1 activity to convert sucrose into glucose signaling to dynamically control the juvenile-to-adult phase transition. Moreover, PAP1 directly binds to the SPL9 promoter, activating SPL9 expression and triggering the sucrose-signaling-mediated juvenile-to-adult phase transition. Therefore, a glucose-signaling feed-forward loop and a sucrose-signaling pathway synergistically regulate the Arabidopsis juvenile-to-adult phase transition. Collectively, we identify a molecular link between the major photosynthate sucrose, the entry point of carbon into cellular metabolism, and the plant juvenile-to-adult phase transition