90 research outputs found
Iron Overload Promotes Arrhythmias via ROS Production and Mitochondrial Membrane Potential Depolarization
ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ ΠΏΠΎΠ»Π½ΡΡ ΡΠ°ΡΡΡΠΎΡΠ½ΠΈΠΉ ΠΈ ΠΌΠ΅Ρ Π½Π΅Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎΡΡΠΈ Π΄Π»Ρ ΡΠΎΡΠΌΡΠ» Π»ΠΎΠ³ΠΈΠΊ ΠΡΠΊΠ°ΡΠ΅Π²ΠΈΡΠ° Π΄Π»Ρ Π°Π²ΡΠΎΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ»Π°ΡΡΠ΅ΡΠΈΠ·Π°ΡΠΈΠΈ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ² Π»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ Π²ΡΡΠΊΠ°Π·ΡΠ²Π°Π½ΠΈΠΉ ΠΈΠ· Π±Π°Π·Ρ Π·Π½Π°Π½ΠΈΠΉ
Π ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ Π»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π²ΡΡΠΊΠ°Π·ΡΠ²Π°Π½ΠΈΡ ΡΠΊΡΠΏΠ΅ΡΡΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΠΌΠΎΠΆΠ½ΠΎ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΡ Π² Π²ΠΈΠ΄Π΅ Π»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΎΡΠΌΡΠ» n-Π·Π½Π°ΡΠ½ΠΎΠΉ Π»ΠΎΠ³ΠΈΠΊΠΈ ΠΡΠΊΠ°ΡΠ΅Π²ΠΈΡΠ°. ΠΡΠΏΠΎΠ»ΡΠ·ΡΡ ΡΠ΅ΠΎΡΠ΅ΡΠΈΠΊΠΎ-ΠΌΠΎΠ΄Π΅Π»ΡΠ½ΡΠΉ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄, Π²Π²Π΅Π΄Π΅Π½Ρ ΠΏΠΎΠ»Π½ΡΠ΅ ΡΠ°ΡΡΡΠΎΡΠ½ΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ ΡΠΎΡΠΌΡΠ»Π°ΠΌΠΈ ΠΈ ΠΌΠ΅ΡΡ Π½Π΅Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎΡΡΠΈ ΡΠΎΡΠΌΡΠ». ΠΠ·ΡΡΠ΅Π½Ρ ΡΠ²ΠΎΠΉΡΡΠ²Π° Π²Π²Π΅Π΄Π΅Π½Π½ΡΡ
Π²Π΅Π»ΠΈΡΠΈΠ½. Π’Π°ΠΊΠΆΠ΅ ΠΏΠΎΠΊΠ°Π·Π°Π½Ρ Π²ΡΡΠΈΡΠ»Π΅Π½ΠΈΡ ΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π²Π΅Π»ΠΈΡΠΈΠ½ Π΄Π»Ρ ΠΊΠ»Π°ΡΡΠ΅ΡΠΈΠ·Π°ΡΠΈΠΈ Π³ΡΡΠΏΠΏ ΡΠΎΡΠΌΡΠ» n-Π·Π½Π°ΡΠ½ΠΎΠΉ Π»ΠΎΠ³ΠΈΠΊΠΈ ΠΡΠΊΠ°ΡΠ΅Π²ΠΈΡΠ° ΠΈΠ· Π±Π°Π·Ρ Π·Π½Π°Π½ΠΈΠΉ (ΡΠΊΡΠΏΠ΅ΡΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ)
FEATURES OF THE USE OF INTRODUCTORY COMPONENTS IN L.N. TOLSTOYβS NOVEL Β«WAR AND PEACEΒ» IN THE LESSONS OF RUSSIAN LANGUAGE AS A FOREIGN
Π Π΄Π°Π½Π½ΠΎΠΉ ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ ΡΠ»ΠΎΠΆΠ½ΠΎΡΡΠΈ, Π²ΠΎΠ·Π½ΠΈΠΊΠ°ΡΡΠΈΠ΅ ΠΏΡΠΈ ΡΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΠΈ Π²Π²ΠΎΠ΄Π½ΡΡ
ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΉ Ρ ΡΡΡΠ΄Π΅Π½ΡΠΎΠ², ΠΈΠ·ΡΡΠ°ΡΡΠΈΡ
ΡΡΡΡΠΊΠΈΠΉ ΡΠ·ΡΠΊ ΠΊΠ°ΠΊ ΠΈΠ½ΠΎΡΡΡΠ°Π½Π½ΡΠΉ. ΠΡΡΠΎΡΠ½ΠΈΠΊΠΎΠΌ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½ΠΈΡ ΠΏΡΠΈΠΌΠ΅ΡΠΎΠ² ΡΠ°ΠΊΠΈΡ
ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΉ Π±ΡΠ΄Π΅Ρ ΡΠΎΠΌΠ°Π½ Π.Π. Π’ΠΎΠ»ΡΡΠΎΠ³ΠΎ Β«ΠΠΎΠΉΠ½Π° ΠΈ ΠΌΠΈΡΒ».=This article is discusses the difficulties that arise from the use of introductory constructions among students studying Russian as a foreign language. The source of consideration of examples of such constructions is the novel of Leo Tolstoy Β«War and PeaceΒ»
FEATURES OF THE USE OF INTRODUCTORY COMPONENTS IN L.N. TOLSTOYβS NOVEL Β«WAR AND PEACEΒ» IN THE LESSONS OF RUSSIAN LANGUAGE AS A FOREIGN
Π Π΄Π°Π½Π½ΠΎΠΉ ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ ΡΠ»ΠΎΠΆΠ½ΠΎΡΡΠΈ, Π²ΠΎΠ·Π½ΠΈΠΊΠ°ΡΡΠΈΠ΅ ΠΏΡΠΈ ΡΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΠΈ Π²Π²ΠΎΠ΄Π½ΡΡ
ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΉ Ρ ΡΡΡΠ΄Π΅Π½ΡΠΎΠ², ΠΈΠ·ΡΡΠ°ΡΡΠΈΡ
ΡΡΡΡΠΊΠΈΠΉ ΡΠ·ΡΠΊ ΠΊΠ°ΠΊ ΠΈΠ½ΠΎΡΡΡΠ°Π½Π½ΡΠΉ. ΠΡΡΠΎΡΠ½ΠΈΠΊΠΎΠΌ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½ΠΈΡ ΠΏΡΠΈΠΌΠ΅ΡΠΎΠ² ΡΠ°ΠΊΠΈΡ
ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΉ Π±ΡΠ΄Π΅Ρ ΡΠΎΠΌΠ°Π½ Π.Π. Π’ΠΎΠ»ΡΡΠΎΠ³ΠΎ Β«ΠΠΎΠΉΠ½Π° ΠΈ ΠΌΠΈΡΒ».=This article is discusses the difficulties that arise from the use of introductory constructions among students studying Russian as a foreign language. The source of consideration of examples of such constructions is the novel of Leo Tolstoy Β«War and PeaceΒ»
Emotional Intelligence as a Factor in Adolescent Hardiness
The article discusses the possibilities of developing emotional intelligence among teachers in order to increase the hardiness of children and adolescents.Π ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠΌΠΎΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠ΅Π»Π»Π΅ΠΊΡΠ° Ρ ΠΏΠ΅Π΄Π°Π³ΠΎΠ³ΠΎΠ² Ρ ΡΠ΅Π»ΡΡ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠΈ ΠΆΠΈΠ·Π½Π΅ΡΡΠΎΠΉΠΊΠΎΡΡΠΈ Π΄Π΅ΡΠ΅ΠΉ ΠΈ ΠΏΠΎΠ΄ΡΠΎΡΡΠΊΠΎΠ²
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Repression of interrupted and intact rDNA by the SUMO pathway in Drosophila melanogaster
Ribosomal RNAs (rRNAs) are essential components of the ribosome and are among the most abundant macromolecules in the cell. To ensure high rRNA level, eukaryotic genomes contain dozens to hundreds of rDNA genes, however, only a fraction of the rRNA genes seems to be active, while others are transcriptionally silent. We found that individual rDNA genes have high level of cell-to-cell heterogeneity in their expression in Drosophila melanogaster. Insertion of heterologous sequences into rDNA leads to repression associated with reduced expression in individual cells and decreased number of cells expressing rDNA with insertions. We found that SUMO (Small Ubiquitin-like Modifier) and SUMO ligase Ubc9 are required for efficient repression of interrupted rDNA units and variable expression of intact rDNA. Disruption of the SUMO pathway abolishes discrimination of interrupted and intact rDNAs and removes cell-to-cell heterogeneity leading to uniformly high expression of individual rDNA in single cells. Our results suggest that the SUMO pathway is responsible for both repression of interrupted units and control of intact rDNA expression
ΠΠ΅Π»Π°Π½ΠΎΠΌΠ° ΠΊΠΎΠΆΠΈ: ΠΎΡ ΡΠΈΡΡΠ΅ΠΌΠ½ΠΎΠΉ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΊ ΠΏΠ΅ΡΡΠΎΠ½ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ
Systematic biology is a new field of biomedicine based on the integrative approach to molecular mechanisms of the operation of living systems including in case of the development of pathological processes. In this connection, up-to-date therapeutic approaches to skin melanoma treatment can be considered on the basis of key changes in intermolecular interactions taking place during tumor development.Π‘ΠΈΡΡΠ΅ΠΌΠ½Π°Ρ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡ ΡΠ²Π»ΡΠ΅ΡΡΡ Π½ΠΎΠ²ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΡΡ Π±ΠΈΠΎΠΌΠ΅Π΄ΠΈΡΠΈΠ½Ρ, Π² ΠΎΡΠ½ΠΎΠ²Π΅ ΠΊΠΎΡΠΎΡΠΎΠΉ Π»Π΅ΠΆΠΈΡ ΠΈΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΠ²Π½ΡΠΉ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ ΠΊ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½ΠΈΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΆΠΈΠ²ΡΡ
ΡΠΈΡΡΠ΅ΠΌ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ ΠΏΡΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΠΈ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ². Π ΡΡΠΎΠΉ ΡΠ²ΡΠ·ΠΈ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Ρ Π² ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ ΠΊΠΎΠΆΠΈ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ ΠΊΠ»ΡΡΠ΅Π²ΡΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ ΠΌΠ΅ΠΆΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ, ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΡΡΠΈΡ
ΠΏΡΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Π΄Π°Π½Π½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ
Dynamics of the chemokine ENA-78/CXCL5 levels in blood serum and skin exudate in patients with atopic dermatitis
Currently, there are only scarce data on dynamics of biologically active substances in the lesions associated with atopic dermatitis. Persistence of microorganisms in atopic dermatitis is high on the skin surface. However, pathophysiological significance of ENA-78/CXCL5 for development of atopic dermatitis was not studied so far. The ENA-78/CXCL5 is known to be produced by endotheliocytes, keratinocytes, eosinophils, fibroblasts to activate neutrophil migration, especially under the influence of LPS-containing microorganisms. The aim of this study was to evaluate the dynamics of ENA-78/CXCL5 chemokine levels in blood serum and skin exudates in the patients with atopic dermatitis, as well as to determine pathophysiological role of the chemokine in pathogenesis of dermatosis. 80 patients with limited and widespread forms of atopic dermatitis and 15 volunteers were under observation. The dynamics of ENA-78/CXCL5 levels was studied in blood sera and skin exudates. Blood samples for the study were drawn at the time periods of exacerbation and remission. Skin exudates were taken from the patients during the exacerbation period using disposable insulin syringes and 20-G disposable needles. In healthy volunteers, the skin exudate was obtained by the βskin windowβ technique as described by V.V. Klimov and coauthors βA method for assessing minimal inflammatory activity of skin in atopic dermatitis in remissionβ. The cell analysis was conducted by flow cytofluorimetry using the LEGEND plex TM Human Proinflammatory Chemokine Panel (USA) according to the manufacturerβs protocol. Serum concentrations of chemokine ENA-78/CXCL5 in adolescents with atopic dermatitis, exceeded the range for healthy volunteers. During remission of dermatitis, the chemokine level did not reach the indices in the control group. In adults, the ENA-78/CXCL5 concentration, both at the onset of symptoms and upon their resolution, was below the control levels. Maximal concentrations of ENA-78/CXCL5 chemokine were detected in the skin exudates. As based on our data on the dynamics of ENA-78/CXCL5 chemokine levels, it could be assumed that this substance may represent a sufficient link in pathogenesis of atopic dermatitis, by causing migration of neutrophils and monocytes to the affected area. The ENA-78/CXCL5 chemokine may be a marker of microbial pathogenesis and cellular damage in atopic dermatitis
Potential Arrhythmogenic Role of TRPC Channels and Store-Operated Calcium Entry Mechanism in Mouse Ventricular Myocytes
Background and Purpose: Store-operated calcium entry (SOCE) is an important physiological phenomenon that extensively mediates intracellular calcium ion (Ca2+) load. It has been previously found in myocytes isolated from neonatal or diseased hearts. We aimed to determine its existence, molecular nature in undiseased hearts and its potential arrhythmogenic implications under hyperactive conditions.Experimental Approach: Ventricular myocytes isolated from adult FVB mice were studied by using Ca2+ imaging and whole-cell perforated patch-clamp recording. In addition, lead II ECGs were recorded in isolated Langendorff-perfused mice hearts. Functional TRPC channel antibodies and inhibitors, and TRPC6 activator hyperforin were used.Key Results: In this study, we demonstrate the existence and contribution of SOCE in normal adult mouse cardiac myocytes. For an apparent SOCE activation, complete depletion of sarcoplasmic reticulum (SR) Ca2+ by employing both caffeine (10 mM) and thapsigargin (1 ΞΌM) or cyclopiazonic acid (10 ΞΌM) was required. Consistent with the notion that SOCE may be mediated by heteromultimeric TRPC channels, SOCEs observed from those myocytes were significantly reduced by the pretreatment with anti-TRPC1, 3, and 6 antibodies as well as by gadolinium, a non-selective TRPC channel blocker. In addition, we showed that SOCE may regulate spontaneous SR Ca2+ release, Ca2+ waves, and triggered activities which may manifest cardiac arrhythmias. Since the spontaneous depolarization in membrane potential preceded the elevation of intracellular Ca2+, an inward membrane current presumably via TRPC channels was considered as the predominant cause of cellular arrhythmias. The selective TRPC6 activator hyperforin (0.1β10 ΞΌM) significantly facilitated the SOCE, SOCE-mediated inward current, and calcium load in the ventricular myocytes. ECG recording further demonstrated the proarrhythmic effects of hyperforin in ex vivo mouse hearts.Conclusion and Implications: We suggest that SOCE, which is at least partially mediated by TRPC channels, exists in adult mouse ventricular myocytes. TRPC channels and SOCE mechanism may be involved in cardiac arrhythmogenesis via promotion of spontaneous Ca2+ waves and triggered activities under hyperactivated conditions
Time course of autoantibodies to collagen type I and III in blood serum and skin exudate in atopic dermatitis
In accordance with Clinical Guidelines of the Russian Society of Dermatovenerologists and Cosmetologists, atopic dermatitis is a chronic allergic genetically determined dermatosis of a multifactorial nature. There are, however, some aspects that challenge the allergic nature of dermatosis. For example, according to literature data, not all the patients have increased synthesis of immunoglobulin E, some of them are torpid to antihistamine treatment, and, when examining the skin of some patients with atopic dermatitis, an absolute polymorphism of rashes is revealed, thus being not typical to the reagin-type allergic reactions. According to modern data, autoimmune theory is assumed for the mechanisms of atopic dermatitis. However, objective proofs of this theory have not been presented, thus drawing our attention to the studies of this issue. The aim of this study was to identify autoimmune pathogenetic mechanisms of atopic dermatitis. The study included 40 adolescents and 40 adult patients with limited and extended forms of atopic dermatitis. The patients were evaluated during the period of exacerbation and remission of the disease. Blood and skin exudates samples were taken from all the patients. The control group consisted of 30 practically healthy volunteers in whom skin exudate was obtained by the βskin windowβ technique as proposed by Klimov V.V. et al. βA method for assessing minimal inflammatory activity of skin in atopic dermatitis in remissionβ. Concentrations of IgG autoantibodies to collagen types I and III were determined in blood serum and skin exudate samples applying ELISA techniques with ready-made panels AEA571Hu ELISA Kit for Anti-Collagen Type I Antibody (USA), AEA176Hu ELISA Kit for Anti-Collagen Type III Antibody (USA), according to the manufacturerβs protocols. For the first time, the contents of autoantibodies to skin collagen types I and III in the patients with atopic dermatitis we studied in parallel, i.e., at systemic level and in affected skin. If compared to the group of healthy volunteers, the concentration of autoantibodies to collagen types I and III was found to be increased in all the patients with atopic dermatitis, both during exacerbation and in remission of the disease. The maximal values of autoantibodies to collagen types I and III were recorded in blood serum upon development of clinical symptoms of dermatosis, along with low contents of these antibodies detectable in their skin exudates. Permanently high concentrations of autoantibodies to collagen types I and III in blood serum at exacerbation and remission of atopic dermatitis, and their low level in their skin exudate suggest emergence of circulating and precipitating immune complexes, thus allowing us to consider atopic dermatitis as an autoimmune process
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