Nanoparticle-based techniques for bladder cancer imaging: a review

Bladder cancer is very common in humans and is often characterized by recurrences, compromising the patient's quality of life with a substantial social and economic impact. Both the diagnosis and treatment of bladder cancer are problematic due to the exceptionally impermeable barrier formed by the urothelium lining the bladder; this hinders the penetration of molecules via intravesical instillation while making it difficult to precisely label the tumor tissue for surgical resection or pharmacologic treatment. Nanotechnology has been envisaged as an opportunity to improve both the diagnostic and therapeutic approaches for bladder cancer since the nanoconstructs can cross the urothelial barrier and may be functionalized for active targeting, loaded with therapeutic agents, and visualized by different imaging techniques. In this article, we offer a selection of recent experimental applications of nanoparticle-based imaging techniques, with the aim of providing an easy and rapid technical guide for the development of nanoconstructs to specifically detect bladder cancer cells. Most of these applications are based on the well-established fluorescence imaging and magnetic resonance imaging currently used in the medical field and gave positive results on bladder cancer models in vivo, thus opening promising perspectives for the translation of preclinical results to the clinical practice

Unsupervised analysis of small animal dynamic Cerenkov luminescence imaging

Clustering analysis (CA) and principal component analysis (PCA) were applied to dynamic Cerenkov lumi- nescence images (dCLI). In order to investigate the per- formances of the proposed approaches, two distinct dy- namic data sets obtained by injecting mice with 32 P-ATP and 18 F-FDG were acquired using the IVIS 200 optical im- ager. The k-means clustering algorithm has been applied to dCLI and was implemented using interactive data lan- guage 8.1. We show that cluster analysis allows us to ob- tain good agreement between the clustered and the corre- sponding emission regions like the bladder, the liver, and the tumor. We also show a good correspondence between the time activity curves of the different regions obtained by using CA and manual region of interest analysis on dCLIT and PCA images. We conclude that CA provides an auto- matic unsupervised method for the analysis of preclinical dynamic Cerenkov luminescence image data. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). (DOI: 10.1117/1.3663442

Photodynamic therapy using Cerenkov and radioluminescence light

In this short review the potential use of Cerenkov radiation and radioluminescence as internal sources for Photodynamic therapy (PDT) is discussed. PDT has been developed over the course of more than 100 years and is based on the induced photo conversion of a drug called photosensitizer (PS) that triggers the production of cytotoxic reactive oxygen species (ROS) leading to the killing of the cells. In order to overcome the problem of light penetration in the tissues, different solutions were proposed in the past. The use of radioisotopes like: F-18, Cu-64, Y-90, Lu-177 as internal light sources increase the light fluence at the PS compared to an external source, resulting in a larger cytotoxic effect

Dynamic of lipid droplets and gene expression in response to beta-aminoisobutyric acid treatment on 3T3-L1 cells

Research on adipobiology has recognized the browning process of white adipocytes as a potential therapeutic strategy for the treatment of obesity and related morbidities. Physical exercise stimulates the secretion of myokines, such as \uf062-aminoisobutyric acid (BAIBA), which in turn promotes adaptive thermogenesis. White adipocyte conversion to brown cells involves dynamic changes in lipid droplet (LD) dimension and in the transcription of brown-specific marker genes. This study analyzes the effect of different doses of BAIBA and at different days of development on 3T3-L1 cells by evaluating morphological changes in LDs and the expression of browning gene markers. Results suggested that the highest concentration of BAIBA after 4 days of differentiation produced the most significant effects. The number of LDs per cell increased in comparison to control cells, whereas the surface area significantly decreased. Brown adipocyte markers were up-regulated, but the effect of treatment was lost at 10 days of differentiation. The thermogenic program induced by BAIBA may reflect a rapid adaptation of adipose tissue to physical exercise. This connection stresses the beneficial impact of physical exercise on metabolic health

Evaluación de papa (Solanum tuberosum L.) transgénica con el receptor EFR en respuesta a marchitez bacteriana considerando aspectos en bioseguridad

Programa de Posgrad

Monte Carlo simulations support non-Cerenkov radioluminescence production in tissue

There is experimental evidence for the production of non-Cerenkov radioluminescence in a variety of materials, including tissue. We constructed a Geant4 Monte Carlo simulation of the radiation from P32 and Tc99m interacting in chicken breast and used experimental imaging data to model a scintillation-like emission. The same radioluminescence spectrum is visible from both isotopes and cannot otherwise be explained through fluorescence or filter miscalibration. We conclude that chicken breast has a near-infrared scintillation-like response with a light yield three orders of magnitude smaller than BGO

Overview of the optical properties of fluorescent nanoparticles for optical imaging

Nanoparticles (NPs) include a wide group of small structures composed by very different materials and characterized by peculiar properties that make them suitable for many applications, especially imaging and drug delivery. In this overview we focus on the optical properties of fluorescent NPs available for in vivo, in vitro and ex vivo preclinical studies and detectable with the optical imaging technique alone or in combination with microscopic confocal imaging. We summarize here the basic principles of the optical detection of fluorescent NPs, elucidate which are the current issues to be resolved and possible solutions to achieve the highest sensitivity and specificity for an unbiased analysis. So far NPs application in clinic is in evaluation due to safety questions still unaddressed but in the future they could dramatically improve both preclinical research and patient clinical care

Hyaluronic acid-based nanocomplexes as an innovative therapeutic tool to treat myotonic dystrophy

Hyaluronic acid-based nanocomplexes have been synthesized and tested as an innovative therapeutic tool to treat myotonic dystroph

Optical imaging of Tc-99m-based tracers: in vitro and in vivo results.

It has been recently shown that optical imaging (OI) methods can be used to image the in vivo biodistribution of several radiopharmaceuticals labeled with beta or alpha emitters. In this work particular attention has been focused on investigating the weaker optical signal induced by an almost pure gamma emitter like Tc-99m. Visible light emission measurements of a water solution containing Tc-99m were performed using a small animal OI system. A sequence of images was acquired for 24 h in order to study the decay of the luminescence signal. The difference between the luminescence decay half life and well-known Tc-99m half life was equal to 1%. in vivo imaging was performed by injecting one control nude mice with Tc-99m-MDP. Optical images obtained with equipment designed for bioluminescence imaging showed that a visible light emission was distinguishable and correctly localized in the bladder region where a higher concentration of Tc-99m-MDP was expected. The bladder to background ratio was always greater than 1. We conclude that the experimental data presented in this paper show that it is possible to detect in vivo luminescence optical photons induced by Tc-99m. This is important especially considering the large number of Tc-99m-based radiopharmaceutical currently available

The 1966-1967 Outburst of V1647 Orionis and the Appearance of McNeil's Nebula

We present the results of an investigation aimed at characterizing previous eruptions of V1647 Orionis, the illuminating star of McNeil's Nebula. Photographic plates and films from the Asiago Observatory (1960-1998) and Harvard Observatory (1898-1974) collections were inspected for the nebula. We have determined that, to the plate limits, the known eruption of 1966-1967 is the only one detected during the 38 yr period covered by the Asiago archive. In the Harvard collection, we did not find any additional occurrences in approximately 400 plates of the region taken over the last 100 yr. Based on the Asiago material, we here present a study of the 1966-1967 event. McNeil's Nebula and V1647 Ori are clearly visible on 19 plates obtained with the Asiago 67/92 cm Schmidt telescope from 1966 October 22 to 1967 March 4. The object is not seen on plates taken in 1966 March nor in 1967 November, thus setting a minimum duration time for the eruptive event of 5 months and a maximum of 20 months. The 19 plates showing McNeil's Nebula were digitized, and a morphological and photometric analysis of the object was undertaken. These data show temporal photometric variability, as well as structural differences between different color bands. We finally compare the 1966-1967 event to the recent 2003-2006 eruption and consider the nature of V1647 Ori within the framework of EX Lupi- and FU Orionis-type eruptions