Measured Tan Delta (plain colour circles) versus strain and the final model from the lmer model with fixed effects (solid colour lines) for both <i>Linum usitatissimum</i> cv. Drakkar and cv. Eden.

<p>All parameters were log-transformed. Legend: type is type of wound (C: Control/no wound, L: Longitudinal wound, T: Transversal wound).</p

Schematic drawing of the sampling area in relation to the wound in order to perform the DMA tests.

<p>(A) sampling area of a transversal wounded stem and (B) sampling area of a longitudinal wounded stem.</p

Measured storage modulus (plain colour circles) versus strain and the final model from the lmer model with fixed effects (solid colour lines) for both <i>Linum usitatissimum</i> cv. Drakkar and cv. Eden.

<p>All parameters were log-transformed. Legend: type is type of wound (C: Control/no wound, L: Longitudinal wound, T: Transversal wound).</p

Detail of the data structure of <i>Linum usitatissimum</i> cv. Drakkar and cv. Eden showing the fixed factors and random factors with crossed and nested data.

<p>Detail of the data structure of <i>Linum usitatissimum</i> cv. Drakkar and cv. Eden showing the fixed factors and random factors with crossed and nested data.</p

Schematic drawing of wound types.

<p>The two types of mechanical damage performed with a razor blade on <i>Linum</i> stems showing (a) longitudinal wound with a cut parallel to the fibres and (b) transversal wound with a cut perpendicular to the fibres.</p

Measured stiffness (plain colour circles) versus strain and the final model from the lmer model with fixed effects (solid colour lines) for both <i>Linum usitatissimum</i> cv. Drakkar and cv. Eden.

<p>All parameters were log-transformed. Legend: type is type of wound (C: Control/no wound, L: Longitudinal wound, T: Transversal wound).</p

Stem cross sections of <i>Linum usitatissimum</i> showing wound tissue lignification.

<p>(A-C) Stem cross section of a transversal wounded stem and (D-E) stem cross section of a longitudinal wounded stem. Staining: (A,B,D) Fuchsin-Chrysoidin-Astrablue, (C,E) Phloroglucinol.</p

Average (Least squares means) mechanical properties and morphological traits for each wound type presented with 95% confidence interval for both <i>Linum usitatissimum</i> cultivars.

<p>Calculated from the final linear mixed effect model (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0185958#pone.0185958.s001" target="_blank">S1 Fig</a> in Supporting Information) taking into account both fixed and random effects. The mechanical properties were obtained with a Strain sweep from 0.003% up to 0.14%.</p

Estimates of the fixed effects from the final linear mixed effects models fitted against type of wound, cultivar and amplitude.

<p>Non-significant terms were removed. Random i.e. sample effects were retained (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0185958#pone.0185958.s001" target="_blank">S1 Fig</a> in Supporting Information). The approximation of degrees of freedom is Satterthwaite's. LogSTRAIN is Log(Strain), type is type of wound (L: Longitudinal wound, T: Transversal wound), cv is cultivar of <i>Linum usitatissimum</i> (ED: cv. Eden), SE is standard error.</p

Wound morphology and stem cross sections panel of <i>Linum usitatissimum</i> cv. Drakkar.

<p>Stems at 95 DAS (A-E) control stem without wound, (F-N) 26-day-old wound parallel to the fibres and (O-S) 26-day-old wound transversal to the fibres. Staining: (B,E,G,J,K,N,P,S) Safranin, Acid Yellow and Methyleneblue. (C,D,H,I;L,M,Q,R) Acridine Orange. Scale bars: (A,F,O) = 2 mm, (D,E,I,J,M,N,R,S) = 100 μm.</p