An excess of goodness? Volunteering among Aid professionals in Cambodia

This paper explores the meaning of volunteering among professional aid workers. While they experience disenchantment in their daytime work, volunteering provides them with benefits lacking in their paid jobs. At the same time, a compensatory model does not capture the complex dimensions of this relationship. One motive behind their professional work – bringing about positive change for others - is also the driving force behind their voluntary practices. Such excess of doing good may be indicative of their overall commitment. If aid workers make sense of their actions within a framework of alienated labour, rendering their waged aid work as a commodity, volunteering emerges as a remedial response. At the same time, their paid and unpaid work is animated by the impulse of giving. Such co-existence implies that gifts and commodities are not mutually exclusive; or indeed that both can be understood, following Parry (1986), as emerging from a highly developed capitalist system

Unpacking distinction within mobility : social prestige and international students

Author thanks the Social Sciences and Humanities Research Council of Canada (SSHRC) for their funding on this projecThis paper investigates the complex ways in which young people engage in social distinction within international mobility. The study offers novel conceptual and empirical insights by examining how distinction and social advantage is reproduced through short‐term student mobility from the Global North to the Global South. In doing so, it elucidates the iterative process of distinction‐making within mobility and argues that young mobile people negotiate a tension between different forms of distinction. Specifically, it unpacks and conceptualises distinction into dual categories—collective and individual—and suggests that students alternate and waver between these categories in order to both validate and elevate their position within a mobility hierarchy. The paper also considers how particular places are viewed as more distinctive and affording greater gains in cultural and symbolic capital. It concludes with future interrogations and ways forward for research on international mobility and distinction.PostprintPeer reviewe

A glimpse on Staphylococcus aureus translation machinery and its control

© 2016, Pleiades Publishing, Inc.Staphylococcus aureus is a major opportunistic and versatile pathogen. Because the bacteria rapidly evolve multi-resistances towards antibiotics, there is an urgent need to find novel targets and alternative strategies to cure bacterial infections. Here, we provide a brief overview on the knowledge acquired on S. aureus ribosomes, which is one of the major antibiotic targets. We will show that subtle differences exist between the translation at the initiation step of Gram-negative and Gram-positive bacteria although their ribosomes display a remarkable degree of resemblance. In addition, we will illustrate using specific examples the diversity of mechanisms controlling translation initiation in S. aureus that contribute to shape the expression of the virulence factors in a temporal and dynamic manner

An evaluation of the SWATRER and, CERES-Millet models for southwest Niger

The SWATKER (Dierckx el aL, 1986) soil water and CERES-Millet (Godwin el ol.. 1984; Jones el al. 1984) growth models wcre evaluated for millet (PennisNwn arnuicmun) during the 1989 growing season at Tara, Niger (300 km, south of Niamey). The required minimum data sets for the plant and soil components of each modcl were either f~ld determined or obtained from the literature. A field experiment was carried out in order to validate the soil water balance and plant growth subroutine6 Soil water content. leaf area and dry matter (DM) were measured weekly. Good agreement between simulated and measured soil water content was obtained with SWATRER. The CERES-Millet modcl overestimated leaf area index (LAI). DM and yield by 46%, 40% and 101% respectively. The CERES-Millet modcl consistently overestimated soil water content by 5% throughout the growing season. The SWATRER model showed good promise for evaluating waterilertilizcr management strategies. Once the CERES-Millet modcl is calibrated. it can be utilized to evaluate new production zonq new cultivara and numerous cultural practices

Claims to a nation, dressing the part and other boundary making strategies by skilled migrants in response to ethnic categorization

This article is about self-defined social identities, other people's perceptions of us and the potentially conflictual relationship between these two. Building on a Barthian focus on group boundaries, the article takes the interplay between external categorizations and internal group definitions as its point of departure to examine how individuals negotiate the boundaries of their social identities. Based on a case study of skilled migrants with racialized ethnicities in Finland, I look at how they express their self-defined identity as well-to-do, skilled professionals in the face of contradicting categorizations of them as unskilled , lower-class migrant subjects. I identify two types of complementary approaches employed by the skilled migrants in boundary making strategies to their identity negotiations: those de-emphasizing ethnicity (or its importance), and those emphasizing class status. These approaches are two sides of the same coin; coming from different perspectives, they both aim at a more positively viewed identity, and for individuals to be seen as well-to-do, educated, working professionals, rather than as ethnic migrant subjects. As such, the article also highlights the interconnection of class and ethnicity for the social identities of skilled migrants in Finland.This article is about self-defined social identities, other people’s perceptions of us and the potentially conflictual relationship between these two. Building on a Barthian focus on group boundaries, the article takes the interplay between external categorizations and internal group definitions as its point of departure to examine how individuals negotiate the boundaries of their social identities. Based on a case study of skilled migrants with racialized ethnicities in Finland, I look at how they express their self-defined identity as well-to-do, skilled professionals in the face of contradicting categorizations of them as un-skilled, lower-class migrant subjects. I identify two types of complementary approaches employed by the skilled migrants in boundary making strategies to their identity negotiations: those de-emphasizing ethnicity (or its importance), and those emphasizing class status. These approaches are two sides of the same coin; coming from different perspectives, they both aim at a more positively viewed identity, and for individuals to be seen as well-to-do, educated, working professionals, rather than as ethnic migrant subjects. As such, the article also highlights the interconnection of class and ethnicity for the social identities of skilled migrants in Finland.Peer reviewe

Nucleic Acids Res

Cells adapt to environmental changes by efficiently adjusting gene expression programs. Staphylococcus aureus, an opportunistic pathogenic bacterium, switches between defensive and offensive modes in response to quorum sensing signal. We identified and studied the structural characteristics and dynamic properties of the core regulatory circuit governing this switch by deterministic and stochastic computational methods, as well as experimentally. This module, termed here Double Selector Switch (DSS), comprises the RNA regulator RNAIII and the transcription factor Rot, defining a double-layered switch involving both transcriptional and post-transcriptional regulations. It coordinates the inverse expression of two sets of target genes, immuno-modulators and exotoxins, expressed during the defensive and offensive modes, respectively. Our computational and experimental analyses show that the DSS guarantees fine-tuned coordination of the inverse expression of its two gene sets, tight regulation, and filtering of noisy signals. We also identified variants of this circuit in other bacterial systems, suggesting it is used as a molecular switch in various cellular contexts and offering its use as a template for an effective switching device in synthetic biology studies

Interobserver agreement on definition of the target volume in stereotactic radiotherapy for pancreatic adenocarcinoma using different imaging modalities

PURPOSE The aim of this study was to evaluate interobserver agreement (IOA) on target volume definition for pancreatic cancer (PACA) within the Radiosurgery and Stereotactic Radiotherapy Working Group of the German Society of Radiation Oncology (DEGRO) and to identify the influence of imaging modalities on the definition of the target volumes. METHODS Two cases of locally advanced PACA and one local recurrence were selected from a large SBRT database. Delineation was based on either a planning 4D CT with or without (w/wo) IV contrast, w/wo PET/CT, and w/wo diagnostic MRI. Novel compared to other studies, a combination of four metrics was used to integrate several aspects of target volume segmentation: the Dice coefficient (DSC), the Hausdorff distance (HD), the probabilistic distance (PBD), and the volumetric similarity (VS). RESULTS For all three GTVs, the median DSC was 0.75 (range 0.17-0.95), the median HD 15 (range 3.22-67.11) mm, the median PBD 0.33 (range 0.06-4.86), and the median VS was 0.88 (range 0.31-1). For ITVs and PTVs the results were similar. When comparing the imaging modalities for delineation, the best agreement for the GTV was achieved using PET/CT, and for the ITV and PTV using 4D PET/CT, in treatment position with abdominal compression. CONCLUSION Overall, there was good GTV agreement (DSC). Combined metrics appeared to allow a more valid detection of interobserver variation. For SBRT, either 4D PET/CT or 3D PET/CT in treatment position with abdominal compression leads to better agreement and should be considered as a very useful imaging modality for the definition of treatment volumes in pancreatic SBRT. Contouring does not appear to be the weakest link in the treatment planning chain of SBRT for PACA

Staphylococcus aureus RNAIII Binds to Two Distant Regions of coa mRNA to Arrest Translation and Promote mRNA Degradation

Staphylococcus aureus RNAIII is the intracellular effector of the quorum sensing system that temporally controls a large number of virulence factors including exoproteins and cell-wall-associated proteins. Staphylocoagulase is one major virulence factor, which promotes clotting of human plasma. Like the major cell surface protein A, the expression of staphylocoagulase is strongly repressed by the quorum sensing system at the post-exponential growth phase. Here we used a combination of approaches in vivo and in vitro to analyze the mechanism used by RNAIII to regulate the expression of staphylocoagulase. Our data show that RNAIII represses the synthesis of the protein through a direct binding with the mRNA. Structure mapping shows that two distant regions of RNAIII interact with coa mRNA and that the mRNA harbors a conserved signature as found in other RNAIII-target mRNAs. The resulting complex is composed of an imperfect duplex masking the Shine-Dalgarno sequence of coa mRNA and of a loop-loop interaction occurring downstream in the coding region. The imperfect duplex is sufficient to prevent the formation of the ribosomal initiation complex and to repress the expression of a reporter gene in vivo. In addition, the double-strand-specific endoribonuclease III cleaves the two regions of the mRNA bound to RNAIII that may contribute to the degradation of the repressed mRNA. This study validates another direct target of RNAIII that plays a role in virulence. It also illustrates the diversity of RNAIII-mRNA topologies and how these multiple RNAIII-mRNA interactions would mediate virulence regulation