Juglone Effects by Dual Way on mTOR Gene Expression, Which Plays Central Role in Cell Growth, Survival and Metabolism, in PANC-1 and BxPC-3 Pancreatic Cancer Cell Lines

Background/Aims: Juglone, as a naphthoquinone, has been shown to have cytotoxic and apoptotic effects in various cancer cells and besides this effects it was reported to have anti-invasive and anti-metastatic effects in PANC-1 and BxPC-3 cells in our previous studies. In this study, we investigated the effects of juglone on GRP75, TFAM and mTOR genes encoding key proteins associated with mitochondrial biogenesis and activation in PANC-1 and BxPC-3 pancreatic cancer cells since mitochondria has central roles in cancer cell survival, metastasis and therapeutic resistance. Methods: In our study; 5, 10, 15 and 20 μM juglone doses were selected as the application doses considering the IC50 value determined after MTT test results and the expressions of the target genes were analyzed by qPCR method after application of juglone doses for 24 hours. Results: Our study results revealed that juglone had an opposite and strong effects on mTOR expression in both cell lines. Conclusion: Our findings suggest that juglone has a developable potential and is a promising theurapeutic agent to develop new strategies for the battle with cancer with those effects on mTOR gene which plays a central role in cellular homeostasis and several cellular events including cell growth, survival and metabolism

Incidence and Prognosis of COVID-19 in Patients with Psoriasis: A Multicenter Prospective Study from the Eastern Black Sea Region of Turkey

COVID-19 infection can have a poor prognosis, especial- ly in patients with chronic diseases and those receiving immunosup- pressive or immunomodulating therapies. This study aimed to investigate the severity of COVID-19 infection in patients with psoriasis and compare the infection severity for sys- temic treatments and comorbidities. We conducted a study in the dermatology clinics of five different centers in the Eastern Black Sea region of Turkey. Four hundred and eighty-eight patients were included, and 22.5% were confirmed as having COVID-19 infection. In our study, the frequency of hospitalization rates due to COVID-19 infection were similar (15.4%, 25.9% respectively) in patients receiv- ing biological treatment and receiving non-biological systemic treat- ment (P=0.344). Hospitalization rates were higher in patients with hypertension, androgenetic alopecia, and acitretin use (P=0.043, P=0.028, P=0.040). In conclusion, current biologic treatments and non-biologic system- ic treatments in patients with psoriasis did not appear to increase the risk of the severe form of COVID-19, except for acitretin

Incidence and Prognosis of COVID-19 in Patients with Psoriasis: A Multicenter Prospective Study from the Eastern Black Sea Region of Turkey

COVID-19 infection can have a poor prognosis, especial- ly in patients with chronic diseases and those receiving immunosup- pressive or immunomodulating therapies. This study aimed to investigate the severity of COVID-19 infection in patients with psoriasis and compare the infection severity for sys- temic treatments and comorbidities. We conducted a study in the dermatology clinics of five different centers in the Eastern Black Sea region of Turkey. Four hundred and eighty-eight patients were included, and 22.5% were confirmed as having COVID-19 infection. In our study, the frequency of hospitalization rates due to COVID-19 infection were similar (15.4%, 25.9% respectively) in patients receiv- ing biological treatment and receiving non-biological systemic treat- ment (P=0.344). Hospitalization rates were higher in patients with hypertension, androgenetic alopecia, and acitretin use (P=0.043, P=0.028, P=0.040). In conclusion, current biologic treatments and non-biologic system- ic treatments in patients with psoriasis did not appear to increase the risk of the severe form of COVID-19, except for acitretin

Oral health-related quality of life in elderly and young patients with periodontal diseases

Objective: Due to the increase in the prevalence of periodontal diseases, quality of life decreases with aging. The purpose of this study was to investigate the quality of life and periodontal disease in young adults and elderly individuals. Materials and Method: A total of 104 individuals, including 52 elderly patients with periodontal disease (Test group; 37 with gingivitis and 15 with periodontitis) (mean age 67.56 ± 3.12) and 52 young adult patients with periodontal disease (Control group; 37 with gingivitis and 15 with periodontitis) (mean age 27.88 ± 6.59), were included in the study. The patients’ quality of life was determined by the Oral Health Impact Profile-14 (OHIP-14) questionnaire. Clinical periodontal parameters (gingival index (GI), plaque index (PI), clinical attachment loss (CAL), and probing pocket depth (PPD)) were used in the evaluation. Results: In periodontitis, there was no difference in total OHIP scores between the groups (p>0.05), whereas, in gingivitis, OHIP scores were higher in elderly patients (p<0.05). While GI was significantly higher in elderly patients, PPD and CAL were significantly higher in young adult patients with periodontitis (p<0.05). OHIP sub-groups’ scores for "physical pain, psychological discomfort, and psychological disability" were significantly higher in elderly patients (p<0.05). Conclusion: Oral health-related quality of life was significantly lower in elderly patients with periodontal disease. While gingivitis had a significantly higher effect on the quality of life in elderly patients compared to younger patients, it was observed that there was no difference in both age groups in the presence of periodontitis. Improving oral health with atraumatic and effective treatment applications at early ages can prevent periodontal problems that may occur in advanced ages and increase the quality of life

Evaluation of salivary cortisol levels and perceived stress levels in gingivitis and periodontal healthy individuals

Amaç: Psikososyal faktörler periodontal hastalık için önemli bir risk faktörü olarak tanımlanır, konak direncini değiştirerek ve endokrin dengesizliklere neden olarak periodontal dokuları etkileyebilir. Bu çalışmada algılanan stres ve strese bağlı hormon düzeyiyle periodontal durum arasındaki ilişkinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Bu çalışmaya 42 gingivitisli ve 30 periodontal olarak sağlıklı, toplam 72 birey dahil edildi. Hastaların klinik ve radyolojik periodontal değerlendirmesi yapıldı. Stres düzeyleri tükürük kortizol seviyesi ve Cohen Algılanan Stres Ölçeği (ASÖ-10) değerleri kullanılarak incelendi. Bireylerin tükürükteki kortizol seviyesi ELİSA yöntemi kullanılarak ölçüldü. Elde edilen veriler p<0.05 anlamlılık düzeylerine göre istatistiksel olarak analiz edildi. Bulgular: Sosyodemografik veriler değerlendirildiğinde fırçalama sıklığının kontrol grubunda daha yüksek olduğu görüldü (p<0.05). Periodontal olarak sağlıklı gruptaki tükürük kortizol seviyesi ve ASÖ-10 skorları, gingivitis grubuna kıyasla daha düşüktü (p<0.05). Tükürük kortizol seviyesi ve algılanan stres ölçeği skorları ile periodontal klinik parametreler arasında pozitif korelasyon gözlendi. Sonuç: Gingivitisli bireylerde daha yüksek stres seviyesi görülmektedir. Çalışmamızın sonuçları dahilinde stres ile periodontal hastalık arasında pozitif bir ilişki olduğu söylenebilir. Bununla birlikte, stres ve periodontal hastalık arasındaki ilişkiyi açıklamak için daha ileri araştırmalara ihtiyaç vardır.Aim: Psychosocial factors are identified as animportant risk factor for periodontal disease, affectingperiodontal tissues by altering host resistance andendocrine imbalances. At present study, there wasaimed to evaluate the relationship between perceivedstress scale and stress hormone level and periodontalstatus.Material and Methods: There was included the totalof 72 individuals, 42 subjects with gingivitis and 30periodontal healthy subjects in this study. Clinical andradiological periodontal evaluation of all participantswere performed. Stress levels were assessed usingsalivary cortisol level and Cohen's Perceived StressScale (PSS-10) scores. Salivary cortisol level was measuredusing by ELISA method. The data were analyzedstatistically according to p 0.05 significant levels.Results: When the sociodemographic data werecompared, no statistical difference was found betweenthe groups (p 0.05). Salivary cortisol level and PSS10scores in periodontal healthy subjects were lowerthan subjects with gingivitis (p 0.05). There was apositive correlation between salivary cortisol level andperceived stress scale scores and periodontal clinicalparameters.Conclusion: There were higher levels of stress inindividuals with gingivitis. According to the presentstudy might mention that there is a positiverelationship between stress and periodontal disease.However, the further investigations are needed toexplain the relationship between stress andperiodontal disease

Psoriazis ve periodontal durum arasındaki ilişkinin değerlendirilmesi

Amaç: Periodontal hastalıklar mikrobiyal dental plaktaki bakterilerin neden olduğu kronik bir enfeksiyondur. Periodontopatojen bakteriler hem neden oldukları enflamasyon hem de bakteriyel ürünlerin immün yanıtta oluşturduğu değişikliklerle psoriazis hastalığının seyrini ve şiddetini etkileyebilir. Bu çalışmada psoriazis teşhisi konulmuş olan bireylerle, sistemik olarak sağlıklı bireyler arasındaki periodontal durumun karşılaştırılması amaçlandı.Gereç ve Yöntem: Bu çalışmaya psoriazis tedavisi devam eden 65 birey (test grubu; 29 kadın, 36 erkek) ve sistemik olarak sağlıklı 100 birey (kontrol grubu; 52 kadın, 48 erkek) dahil edildi. Hastaların klinik ve radyolojik periodontal değerlendirmesi yapıldı. Hastalar periodontal durumlarına göre gingivitis, Stage 1 periodontitis ve Stage 2 periodontitis olarak alt gruplara ayrıldı. Psoriazisli bireylerin dermatolojik değerlendirilmesi Psoriazis Alan ve Şiddet İndeksi (PAŞİ) kullanılarak yapıldı. Elde edilen veriler p&lt;0.05 anlamlılık düzeylerine göre istatistiksel olarak analiz edildi.Bulgular: Demografik verilerin değerlendirmesi, gruplar arasında benzer yaş, cinsiyet, medeni durum ve eğitim durumu seviyeleri olduğunu gösterdi (p&gt;0.05). Fırçalama sıklığı, oral hijyen seviyesi ve sosyoekonomik durum kontrol grubunda daha yüksek bulundu (p&lt;0.05). Periodontal klinik parametreler değerlendirildiğinde test grubunda sondalama cep derinliği (SCD) ve plak indeksi (Pİ) seviyelerinin daha yüksek olduğu görüldü(p&lt;0.05). Psoriazisli bireylerde PAŞİ seviyeleri ile periodontal klinik parametreler arası ilişki incelendiğinde ise Pİ, Gİ ve SKİ seviyeleri, PAŞİ değerleri 0-5, 5-10 ve &gt;10 olan bireylerde benzer bulundu (p&gt;0.05). SCD seviyeleri açısından psoriazisli hastaların PASI alt grupları arasında anlamlı farklılık gözlendi(p&lt;0.05).Sonuç: Psoriazis hastalarının, sistemik sağlıklı bireylere göre, ağız hijyeni seviyelerinin ve periodontal durumlarının daha kötü olduğu görülmektedir. Çalışmamızın sonuçları dahilinde psoriazis ile periodontal hastalık arasında pozitif bir ilişki olduğu söylenebilir

Poly(vinyl alcohol)/polyethyleneimine (PVA/PEI) blended monolithic cryogel columns for the depletion of haemoglobin from human blood

We have synthesized PVA/PEI monolithic cryogel columns chelated with Cu2+ ions as a model adsorbent, which is capable of binding haemoglobin (Hb) from human blood. The goal of this study is to perform the depletion of Hb via a single and easy process to be useful in proteomic studies. PVA/PEI-Cu2+ cryogel columns were subjected to adsorption studies of Hb from both aqueous solution and human plasma to evaluate the extent of interaction between cryogel columns and Hb. The effects of experimental parameters, such as pH, Hb equilibrium concentration, adsorption time, temperature, and ionic strength, on Hb adsorption capacity were investigated