Erratum

Evolution des teneurs en polyamines dans les boutons floraux, les fleurs et les jeunes baies de Vitis villifera L. (cv. Cabernet Sauvignon) atteints d'eutypioseVitis 43 (3), 139-144 (2004

Isolation and characterization of an antifungal agent active against human pathogenic fungi, produced by Pseudomonas fluorescens FSJ-3

An antifungal agent, produced by Pseudomonas fiuorescens strain FSJ-3 originating from Moroccan soil (Fès), was isolated and characterized. It was extracted from Sabouraud's glucose broth culture by n-butanol and purified by gel filtration and silica gel chromatography giving yellow crystals. Its structure was assigned to 1-phenazinecarboxamide by analysing IH and 13 C-NMR and mass spectral data. It shOwed excellent activity against several species ofbacteria, yeasts and human pathogenic filamentous fungi , including Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus and caused morphological modifications on Arthroderma simii hyphae. I-phenazinecarboxamide was non toxicwhen tested on rat leucocytes up to the highest concentration (200 pg/ml).Isolement et caractérisation d'une substance antifongique élaborée par Pseudomonas fluorescens FSJ-3, active contre des champignons pathogènes de l'hommeLa souche FSJ-3 de Pseudomonas fZuorescens douée d'une forte activité antifongique contre les champignons pathogènes de l'homme a été isolée à partir du sol (Fès, Maroc). La substance active, élaborée par cette souche, a été extraite du milieu de culture liquide, avec du n-butanol et purifiée par chromatographie sur gel de filtration et sur gel de silice. Le I-phénazinecarboxamide, obtenu sous forme de cristaux jaunes, a été analysé par RMN -IH et I3C et par spectrométrie de masse. Cette molécule inhibe la croissance de plusieurs bactéries, levures et champignons filamenteux, comme Candida albicans, Cryptococcus neoformans , Aspergillus fumigatus. Le 1-phénazinecarboxamide provoque des altérations morphologiques sur les hyphes d' Arthroderma simii. À forte concentration (200 pg/ml), il n'est pas toxique pour les leucocytes de rat

Evolution des teneurs en polyamines dans les boutons floraux, les fleurs et les jeunes baies de Vitis vinifera L. (cv. Cabernet Sauvignon) atteints d’eutypiose

Development of polyamine levels in flower buds, flowers and young berries of Vitis vinifera L. (cv. Cabernet Sauvignon) infected by eutypiosis fungus, Eutypa lata Effects of eutypiosis on polyamines (PAs) and tyramine contents in flower buds, flowers and young berries of grapevine were studied to determine the relationship between these components and disease development. The study was performed using organs (1) from healthy vines, (2) healthy appearing organs from vines with one symptomless arm and one diseased arm, and (3) diseased organs. Free PAs, conjugated polyamines ( PAs-fpm) and wall-bound polyamines (PAs-mm) were analysed in Vitis vinifera L. cv. Cabernet Sauvignon which is sensitive to eutypiosis. An increase of PAs-mm and a decrease of free PAs were observed in diseased organs; particularly putrescine and diaminopropane were concerned. On the other hand these substances were not affected by eutypiosis in the healthy appearing organs. Alterations of free PAs and PAs-mm in diseased organs were correlated with alteration of the flowering process. In contrast, the PAsfpm levels decreased in diseased organs in response to eutypiosis whereas they increased in healthy appearing organs. This group of PAs seems to play an important role in the response to Eutypa lata attacks to prevent the expression of symptoms. Accumulation of the conjugated tyramine in diseased and healthy appearing organs as a response to eutypiosis might be used as marker of Eutypa lata infection

Forme ictérique de la Leptospirose Humaine: présentation d’un cas à El Jadida, Maroc

Leptospirosis has been scarcely described in Morocco and is probably underestimated in the current local medical practices. In October 2010, one patient, 22 years old, was admitted to Mohamed V hospital in El Jadida presenting clinical symptoms assimilable with leptospirosis and hospitalized for biological investigation of leptospirosis markers by serology techniques (Elisa-IgM test and the micro-agglutination reference test) and receiving adequate care and treatment. Seven serogroups of Leptospira have been identified: L. bataviae, L.canicola, L. cynopteri, L. hebdomadis, L.icterohaemorrhagiae, L. patoc (non pathogenic), L. pyrogenes, illustrating a probable wide range of animal reservoir hosts, with predominance of L. icterohaemorrhagiae and L. patoc.Au  Maroc, La  leptospirose est jusqu’à présent peu étudiée et probablement sous- estimée. En octobre 2010, un patient,  âgé de 22 ans, a été admis à l’hôpital Mohamed V d’El Jadida pour un syndrome assimilable à la leptospirose et a été hospitalisé pour recherche des marqueurs biologiques de cette infection par techniques sérologiques (Elisa- IgM et test de microagglutination) et réception  de  soins  et  traitement adéquats. Sept  sérogroupes de Leptospira(L.) ont été identifiés : L. bataviae, L. canicola, L. cynopteri, L.hebdomadis, L.icterohaemorrhagiae, L. patoc (reconnue non pathogène), L. pyrogenes illustrant ainsi la grande diversité des hôtes réservoirs avec prédominance de L.icterohaemorrhagiae et Leptospira patoc