An Unusual Case of Tertiary Syphilis Behaving Like Tongue Squamous Cell Carcinoma.

Syphilis may present with a myriad of oral manifestations in the primary, secondary, and tertiary stages, and may be confused with malignancy. Despite a rise in the incidence of syphilis, tertiary syphilis is exceedingly rare. Tertiary syphilis gummas usually affect the hard palate, while tongue involvement is very rare. A 55-year-old male with extensive smoking and alcohol use was referred for malignancy evaluation with an ulcerative mass creating a tongue cleft, and a positron emission tomography scan suggestive for malignancy. Biopsy results demonstrated no carcinoma but histology demonstrated granulomatous inflammation. Further laboratory results demonstrated elevated rapid plasma reagin titers with Treponema pallidum immunoglobulin G antibodies present. The patient was diagnosed with tertiary syphilis, received appropriate antibiotic therapy, and had healing of the tongue with a persistent cleft. Syphilis may mimic many disease processes. As such, it is important to include this disease in the differential of an unusual tongue lesion. An oral lesion may be the first sign of infection

Molecular recognition of blunt-ended DNA

Many important enzymatic and physical processes occur specifically at the ends of DNA molecules. The goal of this project was to develop a molecule that would be an effective probe of the chemistry a. the ends of DNA. To do this, ethidium bromide, a well-known intercalation (a molecule that binds between DNA base pairs), was blocked to intercalation with bulky t-butyl groups. We proposed that this new molecule would preferentially bind to blunt-ended DNA, where there is much less steric hindrance. The synthesized molecule was characterized by NMR, Fluorescence, UV/Vis, and Mass Spectroscopy. Supporting data were acquired through fluorescence lifetime measurements, which reflect the chemical environment of the fluorophore. Preliminary data indicated that the lifetime of our target molecule lengthened from 3.8 ns to 13.8 ns when the molecule was exposed to short DNA with blunt ends. This effect could not have been due to intercalation, because there was no observed change in the lifer\u3eme of the molecule when it was exposed to long DNA with few blunt ends. Unfortunately, we have not yet been able to duplicate the data supporting blunt-end binding, partially because the association between our target molecule and blunt-ended DNA is extremely small. An interesting aside is that the target molecule converts to another form at low pH and low ionic strength. We believe that it might be stacking on itself to form multimers, but the current evidence is inconclusive. In addition to the fluorescence studies computer modeling was done using Macromodel and Spartan on an SGI workstation. After minimizing the energies of ethidium bromide and the target molecule, several docking studies were done to predict the ability of each molecule to intercalate DNA. Modeling will be used to help select future blunt-end binding target molecules

Preclinical translation of exosomes derived from mesenchymal stem/stromal cells.

Exosomes are nanovesicles secreted by virtually all cells. Exosomes mediate the horizontal transfer of various macromolecules previously believed to be cell-autonomous in nature, including nonsecretory proteins, various classes of RNA, metabolites, and lipid membrane-associated factors. Exosomes derived from mesenchymal stem/stromal cells (MSCs) appear to be particularly beneficial for enhancing recovery in various models of disease. To date, there have been more than 200 preclinical studies of exosome-based therapies in a number of different animal models. Despite a growing number of studies reporting the therapeutic properties of MSC-derived exosomes, their underlying mechanism of action, pharmacokinetics, and scalable manufacturing remain largely outstanding questions. Here, we review the global trends associated with preclinical development of MSC-derived exosome-based therapies, including immunogenicity, source of exosomes, isolation methods, biodistribution, and disease categories tested to date. Although the in vivo data assessing the therapeutic properties of MSC-exosomes published to date are promising, several outstanding questions remain to be answered that warrant further preclinical investigation

Snap-8 mercury corrosion and materials research, volume iii topical report, jun. 1960 - dec. 1962

SNAP-8 materials research - mercury corrosion capsule tests of ferritic alloys for mass transfer, stress corrosion, mode of attack, and mechanical propertie

Exploring the Use of Social Bookmarking Technology in Education: An Analysis of Students’ Experiences using a Course-specific Delicious.com Account

With more than 4.6 million people, mostly undergraduates, enrolling in at least one online course in fall of 2008, students are showing that they are comfortable with the concept of technology in education. Many students in online classes, however still have to deal with the high cost of textbooks and supplemental materials. Online technologies, however, can provide other alternatives to costly coursepacks and textbooks. Faculty and students may be able to replace or supplement coursepacks and textbooks with social bookmarking sites. This study shows how social bookmarking, specifically Delicious.com, can be used in a course to provide an inexpensive answer to the question of rising course materials costs. Through a series of online focus groups, 53 students enrolled in a “Social Media and Public Relations” course revealed their apprehension toward using an unknown technology and discussed their positive and negative experiences with using the course-specific Delicious.com account. Implications for how social bookmarking can impact online and offline learning are discussed

Autofluorescence lifetime augmented reality as a means for real-time robotic surgery guidance in human patients.

Due to loss of tactile feedback the assessment of tumor margins during robotic surgery is based only on visual inspection, which is neither significantly sensitive nor specific. Here we demonstrate time-resolved fluorescence spectroscopy (TRFS) as a novel technique to complement the visual inspection of oral cancers during transoral robotic surgery (TORS) in real-time and without the need for exogenous contrast agents. TRFS enables identification of cancerous tissue by its distinct autofluorescence signature that is associated with the alteration of tissue structure and biochemical profile. A prototype TRFS instrument was integrated synergistically with the da Vinci Surgical robot and the combined system was validated in swine and human patients. Label-free and real-time assessment and visualization of tissue biochemical features during robotic surgery procedure, as demonstrated here, not only has the potential to improve the intraoperative decision making during TORS but also other robotic procedures without modification of conventional clinical protocols

A 9Cr-1Mo steel as a mercury containment material for the SNAP-8 boiler

9Cr-1Mo steel as mercury containment material for SNAP-8 boile

Evaluation of tantalum for mercury containment in the SNAP-8 boiler

Corrosion testing of tantalum for mercury containment in SNAP 8 boile