Guiding Illiterate Parents in Assistning their Children in Emergent Literacy

As the number of illiterate adults continues to grow, increasing numbers of children have parents with limited reading and writing skills. Such children are deprived of the joys of reading, for their parents do not read to them at bedtime nor do such parents peruse the morning paper

Histone Hypervariants H2A.Z.1 and H2A.Z.2 Play Independent and Context-Specific Roles in Neuronal Activity-Induced Transcription of Arc/Arg3.1 and Other Immediate Early Genes.

The histone variant H2A.Z is an essential and conserved regulator of eukaryotic gene transcription. However, the exact role of this histone in the transcriptional process remains perplexing. In vertebrates, H2A.Z has two hypervariants, H2A.Z.1 and H2A.Z.2, that have almost identical sequences except for three amino acid residues. Due to such similarity, functional specificity of these hypervariants in neurobiological processes, if any, remain largely unknown. In this study with dissociated rat cortical neurons, we asked if H2A.Z hypervariants have distinct functions in regulating basal and activity-induced gene transcription. Hypervariant-specific RNAi and microarray analyses revealed that H2A.Z.1 and H2A.Z.2 regulate basal expression of largely nonoverlapping gene sets, including genes that code for several synaptic proteins. In response to neuronal activity, rapid transcription of our model gene Arc is impaired by depletion of H2A.Z.2, but not H2A.Z.1. This impairment is partially rescued by codepletion of the H2A.Z chaperone, ANP32E. In contrast, under a different context (after 48 h of tetrodotoxin, TTX), rapid transcription of Arc is impaired by depletion of either hypervariant. Such context-dependent roles of H2A.Z hypervariants, as revealed by our multiplexed gene expression assays, are also evident with several other immediate early genes, where regulatory roles of these hypervariants vary from gene to gene under different conditions. Together, our data suggest that H2A.Z hypervariants have context-specific roles that complement each other to mediate activity-induced neuronal gene transcription

Prediction of Fretting Crack Location and Orientation in a Single Crystal Nickel Alloy

Fretting is a structural damage mechanism arising between two nominally clamped surfaces subjected to an oscillatory loading. A critical location for fretting induced damage has been identified at the blade/disk and blade/damper interfaces of gas turbine engine turbomachinery and space propulsion components. The high- temperature, high-frequency loading environment seen by these components lead to severe stress gradients at the edge-of-contact that could potentially foster crack growth leading to component failure. These contact stresses drive crack nucleation in fretting and are very sensitive to the geometry of the contacting bodies, the contact loads, materials, temperature, and contact surface tribology (friction). Recently, a high-frequency, high-temperature load frame has been designed for experimentally investigating fretting damage of single crystal nickel materials employed in aircraft and spacecraft turbomachinery. A modeling method for characterizing the fretting stresses of the spherical fretting contact stress behavior in this experiment is developed and described. The calculated fretting stresses for a series of experiments are then correlated to the observed fretting damage. Results show that knowledge of the normal stresses and resolved shear stresses on each crystal plane can aid in predicting crack locations and orientations

Advertising, product quality, and complex evolving marketing systems

The paper analyses the advertising as power vs. advertising as information controversy as well as its recent empirical testing. It is stressed that this distinction focuses too much on the interaction between consumer and manufacturer while ignoring the retailer as an important stake-holder. To compensate for this lack, a complex marketing system perspective is introduced in which consumer, retailer, and manufacturer interact. However, these complex marketing systems might drift towards market equilibria which are against the consumer interests: that is, firmsmight lock out brands from the market by means of trade and sales promotions and then use advertising to protect their position. Consequently brands of better quality and/or innovative brands are barred from trade shelves

Status of QCD

I have been asked to discuss the status of QCD. It seems to me that there are three main points to be made about the present status of QCD: $\bullet$ QCD is right, and we can do many beautiful things with it. $\bullet$ There are several important concrete problems that lie just beyond the edge of our current understanding. $\bullet$ There are some foundational issues in QCD, and some recent developments, that may point toward entirely new directions. These points will, I believe, emerge quite clearly from the following more detailed discussion. The discussion will be in three parts. I'll first discuss elementary processes, then more complicated processes, and then finally foundational issues.Comment: 28 pages, use Phyzzx, figures available by FAX or mail on request, IASSNS-HEP-93/6

The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis

The Ribosomal Database Project (RDP-II) provides the research community with aligned and annotated rRNA gene sequences, along with analysis services and a phylogenetically consistent taxonomic framework for these data. Updated monthly, these services are made available through the RDP-II website (http://rdp.cme.msu.edu/). RDP-II release 9.21 (August 2004) contains 101 632 bacterial small subunit rRNA gene sequences in aligned and annotated format. High-throughput tools for initial taxonomic placement, identification of related sequences, probe and primer testing, data navigation and subalignment download are provided. The RDP-II email address for questions or comments is [email protected]

A Low-Cost Microprocessor-Controlled Stance-Control Knee Orthosis for Pediatric Mobility Impairments

Knee-ankle-foot orthoses (KAFOs) are commonly prescribed for individuals with knee instability or quadriceps weakness resulting from a variety of conditions such as spinal cord injury, stroke, spina bifida, and cerebral palsy. A locking KAFO fixes the leg fully straight and is the most prescribed orthotic intervention for these conditions. However, the functional benefits of lower extremity orthoses are maximally realized in stance-controlled knee-ankle-foot orthoses (SC-KAFOs) which allow free knee motion during swing but lock during stance. Existing SC-KAFO options are generally unreliable or expensive, and thus an unmet need exists for a reliable, versatile orthosis which can be manufactured at relatively low cost. The SC-KO team is working to meet this need by developing an intelligent stance-controlled knee orthotic with an any-angle locking mechanism controlled by a microprocessor based on information received from onboard inertial gait-phase sensing. The resulting device will allow for reliable knee locking for support during the stance phase, easy unlocking even under load for the free swing phase, and predictable, safe behavior on stairs and uneven terrain. The system is being developed as a knee-only orthosis but can be adapted for a full knee-ankle-foot orthosis, with ankle support being prescribed as needed. The first system developed will be configured for pediatric use to address mobility impairments arising from cerebral palsy and spina bifida with CURE Ethiopia serving as the clinical partner for the development and testing. Funding for this work provided by The Collaboratory for Strategic Partnerships and Applied Research.https://mosaic.messiah.edu/engr2022/1017/thumbnail.jp