Recent Progress and Challenges on the Microfluidic Assay of Pathogenic Bacteria Using Biosensor Technology

Microfluidic technology is one of the new technologies that has been able to take advantage of the specific properties of micro and nanoliters, and by reducing the costs and duration of tests, it has been widely used in research and treatment in biology and medicine. Different materials are often processed into miniaturized chips containing channels and chambers within the microscale range. This review (containing 117 references) demonstrates the significance and application of nanofluidic biosensing of various pathogenic bacteria. The microfluidic application devices integrated with bioreceptors and advanced nanomaterials, including hyperbranched nano-polymers, carbon-based nanomaterials, hydrogels, and noble metal, was also investigated. In the present review, microfluid methods for the sensitive and selective recognition of photogenic bacteria in various biological matrices are surveyed. Further, the advantages and limitations of recognition methods on the performance and efficiency of microfluidic-based biosensing of photogenic bacteria are critically investigated. Finally, the future perspectives, research opportunities, potential, and prospects on the diagnosis of disease related to pathogenic bacteria based on microfluidic analysis of photogenic bacteria are provided

DNA based biosensing of Acinetobacter baumannii using nanoparticles aggregation method

Acinetobacter baumannii is the main cause of nosocomial infections in blood, urinary tract, wounds and in lungs leading to pneumonia. Apart from its strong predilection to be the cause of serious illnesses in intensive care units. Herein, we present a specific and sensitive approach for the monitoring of Acinetobacter baumannii genome based on citrate capped silver nanoparticles (Cit-AgNPs) using spectroscopic methods. In this study, (5' SH-TTG TGA ACT ATT TAC GTC AGC ATG C3') sequence was used as a probe DNA (pDNA) of Acinetobacter baumannii. Then, complementary DNA (cDNA) was used for hybridization. After the hybridization of pDNA with cDNA, target DNA (5' GCA TGC TGA CGT AAA TAGTTC ACA A 3') was recognized and detected using turn-on fluorescence bioassay. After the hybridization of pDNA with cDNA, the target DNA was successfully measured in optimum time of 2 min by spectrophotometric techniques. Moreover, the selectivity of designed bioassay was evaluated in the presence of two mismatch sequences and excellent differentiation was obtained. 1 Zepto-molar (zM) of low limit of quantification (LLOQ) was achieved by this genosensor. The present study paved the way for quick (2 min) and accurate detection of Acinetobacter baumannii, which can be a good alternative to the traditional methods. Current study proposed a novel and significant diagnostic test towards Acinetobacter baumannii detection based on silver nanoparticles aggregation which has the capability of being a good alternative to the traditional methods. Moreover, the proposed genosensor successfully could be applied for the detection of other pathogens.Funding Agency: Tabriz University of Medical Sciences </p