Stability of diluted adenosine solutions in polyvinyl chloride infusion bags

Purpose The stability of diluted adenosine solutions in polyvinyl chloride infusion bags was studied. Methods Adenosine 50-, 100-, and 220-μg/mL solutions were prepared in 50-mL polyvinyl chloride (PVC) infusion bags containing 0.9% sodium chloride injection or 5% dextrose injection and stored at room temperature (23–25 °C) or under refrigeration (2–8 °C). Each sample of every combination of concentration, diluent, and storage temperature was prepared in triplicate, yielding 36 samples. The samples were assayed using a stability-indicating, reverse-phase high-performance liquid chromatographic method immediately after preparation (time zero) and at 24 hours, 48 hours, 7 days, and 14 days. pH was measured at time zero, 48 hours, 7 days, and 14 days. Time zero concentrations were calculated from the equation produced from a calibration curve of standards ranging from 10 to 500 μg/mL. Samples were also visually inspected against a light background for clarity, color, and the presence of crystalline particulate matter. Stability was defined as retaining at least 90% of the initial adenosine concentration. Results After 14 days, all samples retained greater than 98% of the initial adenosine concentration, with no evidence of adsorption, visible precipitation, or considerable change in pH, suggesting minimal to no loss of product due to degradation or adsorption. Conclusion Adenosine 50-, 100-, and 220-μg/mL solutions in 50-mL PVC infusion bags containing 0.9% sodium chloride injection or 5% dextrose injection stored at room temperature and refrigerated conditions were stable for at least 14 days

Characteristics of pine needle combustion in a semi-gasifier burner

Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, June 2011."June 2011." Cataloged from PDF version of thesis.Includes bibliographical references (p. 29).The motivation behind this report was to develop a stronger understanding of pine needle combustion behavior in a semi-gasifier, in order to ultimately design an effective pine needle cook stove for people in the developing world. Pine needles are a cheap, energy-dense fuel source that can be harnessed for cooking purposes, but they are often physically or chemically altered prior to being used as a fuel. The challenge remains to develop a stove that uses unprocessed pine needles. Using principles of a semi-gasification biomass stove, this paper explores the combustion and burn characteristics of unprocessed pine needles by measuring the effects of temperature in a configured burner when altering primary and secondary airflow into the burner. Experiments showed that pine needles in a semi-gasifier combust in two regimes--one characterized by secondary combustion of volatiles released from the primary burn of raw pine needles, and another characterized by the smoldering of pine needle charcoal. Each regime needed unique balances between primary air and secondary airflow. The first regime required a relatively low primary airflow with a high secondary airflow, while the second regime needed a high primary airflow to maintain charring and low or no secondary airflow. The balance of airflow in the first regime was crucial in determining whether the volatiles combusted or were released as plumes of smoke. The findings of this report can be used as an initial benchmark and resource in the further development of an existing proof-of-concept prototype, which was built by the authors with a team of MIT students and affiliates and a community partner in Uttarakhand, India.by Liane Jessica Fang and Caroline M. Hane-Weijman.S.B

Talk of the town: August Man Malaysia's roundup of the most controvesial headlines of the year

Before we look forward, let us reminisce about the things that have impacted us the most this year 2013 seemed to be a particular big one: our country had its long-awaited general elections, the U.S government shut down, and really, we've just had plenty of raging controversial issues that continued to hit our headlines one after another We take a look at some of the most talked about issues that have gone viral and sparked massive debate alike

Observing the Effects of Galaxy Interactions on the Circumgalactic Medium

We continue our empirical study of the emission line flux originating in the cool ($T\sim10^4$ K) gas that populates the halos of galaxies and their environments. Specifically, we present results obtained for a sample of galaxy pairs with a range of projected separations, {\bf $10 < {S_p/\rm kpc} < 200$}, and mass ratios $<$ 1:5, intersected by 5,443 SDSS lines of sight at projected radii of 10 to 50 kpc from either or both of the two galaxies. We find significant enhancement in H$\alpha$ emission and a moderate enhancement in [N {\small II}]6583 emission for low mass pairs (mean stellar mass per galaxy, $\overline{\rm M}_*, <10^{10.4} {\rm M}_\odot$) relative to the results from a control sample. This enhanced H$\alpha$ emission comes almost entirely from sight lines located between the galaxies, consistent with a short-term, interaction-driven origin for the enhancement. We find no enhancement in H$\alpha$ emission, but significant enhancement in [N {\small II}]6583 emission for high mass ($\overline{\rm M}_* >10^{10.4}{\rm M}_\odot$) pairs. Furthermore, we find a dependence of the emission line properties on the galaxy pair mass ratio such that those with a mass ratio below 1:2.5 have enhanced [N {\small II}]6583 and those with a mass ratio between 1:2.5 and 1:5 do not. In all cases, departures from the control sample are only detected for close pairs ($S_p <$ 100 kpc). Attributing an elevated [N {\small II}]6583/H$\alpha$ ratio to shocks, we infer that shocks play a role in determining the CGM properties for close pairs that are among the more massive and have mass ratios closer to 1:1.Comment: 6 pages and 3 figure

Treatment patterns and outcomes in patients with Pancoast tumors: a national cancer database analysis

Background: Pancoast tumors represent 5% of non-small cell lung cancers. Complete surgical resection and no lymph node involvement are important positive prognostic factors. Previous literature has identified neoadjuvant chemoradiation treatment, followed by surgical resection, as the standard of care. But many institutions choose upfront surgery. Our goal was to identify the treatment patterns and outcomes in patients with node-negative Pancoast tumors using the National Cancer Database (NCDB). Methods: The NCDB was queried from 2004 through 2017 to identify all patients who had undergone surgery for a Pancoast tumor. Treatment patterns, including the percentage of patients who received neoadjuvant treatment, were recorded. Logistic regression and survival analyses were used to determine outcomes based on different treatment patterns. Secondary analyses were performed on the cohort who received upfront surgery. Results: A total of 2,910 patients were included in the study. Overall 30- and 90-day mortality were 3% and 7% respectively. Only 25% (717/2,910) of the group received neoadjuvant chemoradiation treatment prior to surgery. Patients who received neoadjuvant chemoradiation treatment experienced significantly improved 90-day survival (P\u3c0.01) and overall survival (P\u3c0.01). When analyzing the cohort who received upfront surgery, there was a statistically significant difference in survival based on adjuvant treatment pattern (P\u3c0.01). Patients in this group who received adjuvant chemoradiation had the best survival, whereas patients who received adjuvant radiation only or no treatment had the worst outcomes. Conclusions: Patients with Pancoast tumors receive neoadjuvant chemoradiation treatment in only a quarter of cases nationally. Patients who received neoadjuvant chemoradiation treatment had improved survival compared to patients who had upfront surgery. Similarly, when surgery is performed first, adjuvant chemoradiation treatment improved survival compared to other adjuvant strategies. These results suggest underutilization of neoadjuvant treatment for patients with node-negative Pancoast tumors. Future studies with a more clearly defined cohort are needed to assess the treatment patterns being utilized on patients with node-negative Pancoast tumors. It will be beneficial to see whether neoadjuvant treatment for Pancoast tumors has increased in recent years

Poly-ADP-Ribosylation of Estrogen Receptor-Alpha by PARP1 Mediates Antiestrogen Resistance in Human Breast Cancer Cells

Therapeutic targeting of estrogen receptor-α (ERα) by the anti-estrogen tamoxifen is standard of care for premenopausal breast cancer patients and remains a key component of treatment strategies for postmenopausal patients. While tamoxifen significantly increases overall survival, tamoxifen resistance remains a major limitation despite continued expression of ERα in resistant tumors. Previous reports have described increased oxidative stress in tamoxifen resistant versus sensitive breast cancer and a role for PARP1 in mediating oxidative damage repair. We hypothesized that PARP1 activity mediated tamoxifen resistance in ERα-positive breast cancer and that combining the antiestrogen tamoxifen with a PARP1 inhibitor (PARPi) would sensitize tamoxifen resistant cells to tamoxifen therapy. In tamoxifen-resistant vs. -sensitive breast cancer cells, oxidative stress and PARP1 overexpression were increased. Furthermore, differential PARylation of ERα was observed in tamoxifen-resistant versus -sensitive cells, and ERα PARylation was increased by tamoxifen treatment. Loss of ERα PARylation following treatment with a PARP inhibitor (talazoparib) augmented tamoxifen sensitivity and decreased localization of both ERα and PARP1 to ERα-target genes. Co-administration of talazoparib plus tamoxifen increased DNA damage accumulation and decreased cell survival in a dose-dependent manner. The ability of PARPi to overcome tamoxifen resistance was dependent on ERα, as lack of ERα-mediated estrogen signaling expression and showed no response to tamoxifen-PARPi treatment. These results correlate ERα PARylation with tamoxifen resistance and indicate a novel mechanism-based approach to overcome tamoxifen resistance in ER+ breast cancer

A global view of aquaculture policy

Aquaculture is among the most dynamic sectors in the global food system, yet it remains surprisingly underrepresented in the mainstream literature on food policy. This article reviews 204 published articles and re­ ports and shows that government policies have strongly influenced the geographic distribution of aquaculture growth, as well as the types of species, technology, management practices, and infrastructure adopted in different locations. Global cross-section studies reveal a broad spectrum of under- to over-regulated aquaculture systems that correspond, respectively, to high- and low-growth areas for aquaculture. The bulk of this paper centers on aquaculture policy as it plays out six individual countries plus the EU: Bangladesh, Zambia, Chile, China, USA, and Norway. These case studies shed light on aquaculture policies aimed at economic development, aquaculture disease management, siting, environmental performance, and trade protection. Experiences from these countries point to the need to find the right policy balance between semi-subsistence farms, small and medium enterprises (SME), and large-scale commercial operations, particularly in low-income settings. The cases also highlight the importance of addressing aquaculture disease pressures and misuse of antimicrobials in many parts of the world, and identifying successful aquaculture policy instruments and institutions that can be transferred between countries. The review underscores the challenges of establishing nutrition-sensitive aquaculture policies and of incorporating aquaculture directly into food policy and global food system dialogues and action

Tree species traits but not diversity mitigate stem breakage in a subtropical forest following a rare and extreme ice storm

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