TELEX HEBDOMADAIRE NR 95 DU 17.09.82 DESTINE A L'ENSEMBLE DES DELEGATIONS EXTERIEURES ET BUREAUX DE PRESS ET D'INFORMATION INDEPENDANTS DANS LES PAYS TIERS = WEEKLY MEMO NO. 95 FOR 17.09.82 TO FOREIGN DELEGATIONS AND PRESS BUREAUS OF THIRD COUNTRIES

<p>High-performance liquid chromatography (HPLC) results of (A) commercial surfactin sample, and (B) our extract surfactin of <i>B</i>. <i>subtilis</i> HH2 in LB medium. There were three main peaks (Peak A-C) of the extract and the surfactin standard in the same location.</p

Microwave Spectrum and Molecular Structure of Vinyl Chloride–Acetylene, A Side Binding Complex

The structure of the gas-phase bimolecular complex formed between vinyl chloride and acetylene is determined using a combination of broad-band, chirped-pulse, and narrow-band, Balle–Flygare Fourier transform microwave spectroscopy from 5.8 to 20.7 GHz. Although all previous examples of complexes formed between protic acids and haloethylenes are observed to have similar modes of binding regardless of the specific identity of the acid, HF, HCl, or HCCH, the vinyl chloride–HCCH complex has HCCH located at one end of the vinyl chloride with the secondary interaction occurring with the geminal hydrogen atom as opposed to the “top” binding configuration found for vinyl chloride–HF. Nevertheless, the details of the structure, such as hydrogen bond length (3.01 Å) and amount of deviation from linearity (58.5°), do reflect the strength of the interaction and show clear correlations with the gas-phase acidity. Comparison with analogous complexes allows the determination of the relative importance of electrostatic interactions and steric requirements in leading to the observed structures

Presentation_1_Nitric Oxide Affects Rice Root Growth by Regulating Auxin Transport Under Nitrate Supply.pdf

<p>Nitrogen (N) is a major essential nutrient for plant growth, and rice is an important food crop globally. Although ammonium (NH₄⁺) is the main N source for rice, nitrate (NO₃^-) is also absorbed and utilized. Rice responds to NO₃^- supply by changing root morphology. However, the mechanisms of rice root growth and formation under NO₃^- supply are unclear. Nitric oxide (NO) and auxin are important regulators of root growth and development under NO₃^- supply. How the interactions between NO and auxin in regulating root growth in response to NO₃^- are unknown. In this study, the levels of indole-3-acetic acid (IAA) and NO in roots, and the responses of lateral roots (LRs) and seminal roots (SRs) to NH₄⁺ and NO₃^-, were investigated using wild-type (WT) rice, as well as osnia2 and ospin1b mutants. NO₃^- supply promoted LR formation and SR elongation. The effects of NO donor and NO inhibitor/scavenger supply on NO levels and the root morphology of WT and nia2 mutants under NH₄⁺ or NO₃^- suggest that NO₃^--induced NO is generated by the nitrate reductase (NR) pathway rather than the NO synthase (NOS)-like pathway. IAA levels, [³H] IAA transport, and PIN gene expression in roots were enhanced under NO₃^- relative to NH₄⁺ supply. These results suggest that NO₃^- regulates auxin transport in roots. Application of SNP under NH₄⁺ supply, or of cPTIO under NO₃^- supply, resulted in auxin levels in roots similar to those under NO₃^- and NH₄⁺ supply, respectively. Compared to WT, the roots of the ospin1b mutant had lower auxin levels, fewer LRs, and shorter SRs. Thus, NO affects root growth by regulating auxin transport in response to NO₃^-. Overall, our findings suggest that NO₃^- influences LR formation and SR elongation by regulating auxin transport via a mechanism involving NO.</p

FBI-1 Enhances ETS-1 Signaling Activity and Promotes Proliferation of Human Colorectal Carcinoma Cells

<div><p>In this study, we investigated a potential regulatory role of FBI-1 in transcription factor activity of ETS-1. The protein interaction was identified between ETS-1 and FBI-1 in lovo cells. The accumulating data showed that FBI-1 promoted the recruitment of ETS-1 to endogenous promoter of its target genes and increase ETS-1 accumulation in the nuclear. Our work also indicated that the FBI-1 enhances ETS-1 transcription factor activity via down-regulating p53-mediated inhibition on ETS-1. Further, FBI-1 plays a role in regulation of colorectal carcinoma cells proliferation. These findings supported that FBI-1 might be a potential molecule target for treating colorectal carcinoma.</p></div

Effect of FBI-1 on ETS-1 cytoplasmic/nucleus localization.

<p>(A–B) The cells were fractionated into cytoplasmic and nuclear fractions. The fractions were examined with anti-ETS-1 antibody, anti-P53 antibody and anti-FBI-1 antibody. The Lamin A/C and tubulin were used as the nuclear and cytoplasmic indicator, respectively.</p

FBI-1 would potentially interact with ETS-1 and P53 in vivo.

<p>(A) Interaction of FLAG-FBI-1 with exogenous ETS-1 or p53 in vivo. Lovo cells were transfected with FLAG-tagged FBI-1 or FLAG empty vector. Then, cell lysates were immunoprecipitated (IP) by anti-FLAG beads, and the precipitates were then immunoblotted (IB) with anti-FLAG antibody, anti-ETS-1 antibody, or anti-p53 antibody. (B) Lovo cells were transfected with FLAG-ETS-1 vector or FLAG empty vector. The IP analysis was performed with anti-FLAG antibody, and the IB analysis was performed with anti-FLAG antibody, anti-FBI-1 antibody, or anti-P53 antibody.</p

The proposed models for certain roles of FBI-1 function in Lovo cells.

<p>ETS-1 would be activated in presence of HGF and be blocked by ARQ-197. FBI-1 may modulate ETS-1 activity through potential protein interaction, recruitment to endogenous MMP1 promoter, or cytoplasmic/nucleus translocation. The regulatory effect of FBI-1 on ETS-1 is also through regulating P53 activity.</p

FBI-1 can enhance the recruitment of ETS-1 to the MMP1 promoter.

<p>(A) Lovo cells stably transfected with FBI-1 or empty vector were prepared and subjected to ChIP by using IgG antibody (negative control) or antibodies for ETS-1, FBI-1 and p53. The Immunoprecipitated DNA fragment was quantified by real-time PCR assay. (B) Lovo cells, which were stably transfected with FBI-1 siRNA, or control siRNA, were harvested for the ChIP assays. The ChIP assays were performed with IgG antibody (negative control) or antibodies for ETS-1, FBI-1 and p53. *P<0.05 versus the empty vector or the FBI-1 vector (A); or versus the control siRNA or the FBI-1 siRNA (B). The cloned promoter region of MMP1 is showed above the figure.</p

FBI-1 modulates ETS-1 downstream genes expression in Lovo cells which were stably transfected with plasmids.

<p>(A-B) Western blotting with various antibodies showed the overexpression of FBI-1 or specific knockdown effect of FBI-1 siRNA on the endogenous FBI-1 protein level. Lovo cells were harvested for WB assays and detected by anti-FBI-1 antibody, anti-MMP1 antibody, anti-MMP9 antibody, anti-u-PA antibody, anti-c-Met antibody, anti-ETS-1 antibody, or anti-GAPDH antibody.</p

FBI-1 promotes colorectal carcinoma cells proliferation in vitro.

<p>(A–B) LoVo, (C–D) HR8348, and (E–F) HT29 cells were stably transfected with the plasmids. Then, relative cell numbers were determined by the MTT assay. Relative cell numbers (A and B) shown are Mean± SD of triplicate measurements and have been repeated 3 times with similar O.D. value results. *P<0.05 versus the empty vector or the FBI-1 vector (A, C, E), *P<0.05 versus the FBI-1 siRNA vector or the control siRNA vector (B, D, F).</p