FBI-1 can enhance the recruitment of ETS-1 to the MMP1 promoter.

Abstract

<p>(A) Lovo cells stably transfected with FBI-1 or empty vector were prepared and subjected to ChIP by using IgG antibody (negative control) or antibodies for ETS-1, FBI-1 and p53. The Immunoprecipitated DNA fragment was quantified by real-time PCR assay. (B) Lovo cells, which were stably transfected with FBI-1 siRNA, or control siRNA, were harvested for the ChIP assays. The ChIP assays were performed with IgG antibody (negative control) or antibodies for ETS-1, FBI-1 and p53. *P<0.05 versus the empty vector or the FBI-1 vector (A); or versus the control siRNA or the FBI-1 siRNA (B). The cloned promoter region of MMP1 is showed above the figure.</p

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