Comparing thrB gene sequencing and multiplex PCR method in grouping of the different strains of escherichia coli

Background and purpose: The best-known groups of Escherichia coli are B2, B1, A, and D. Previous studies have clearly shown the difference between this group such as resistance to antibiotics, the growth rate, and pathogenicity. Therefore, identifying different groups of E. coli could be of great benefit in curing infections caused by this bacterium. There are some defects in traditional diagnosis methods for E. coli groups, hence, this study aimed at finding a more efficient method. Material and methods: To identify different E. coli groups, after surveying genomic sequence of 60 E. coli and their grouping which was done by silico multiplex PCR method, a polymorphic sequence of homoserine kinase enzyme was selected. Then, 20 unknown samples of E. coli strains were grouped by this sequence and multiplex PCR methods. Results: The 20 unknown samples of E. coli strains were grouped identically in both methods. Conclusion: Identification and grouping of different E. coli strains by sequencing method was found to be more precise than multiplex PCR method. © 2014, Mazandaran University of Medical Sciences. All rights reserved

The effect of Phoenix dactylifera pollen on the expression of NRF2, SOD2, CAT, and GPX4 genes, and sperm parameters of fertile and infertile men: A controlled clinical trial

Background: Oxidative stress is caused by the imbalance occurring between the creation and clearance of the reactive oxygen species (ROS), which is responsible for 30–40% of male infertility. The positive impact of phoenix dactylifera pollen (Date palm pollen, DPP) on the improvement of sperm parameters has been well documented in animal models. Objective: For evaluating the effect(s) of DPP on sperm parameters, ROS levels, expression of antioxidant genes, and activity of antioxidant enzymes of infertile men. Materials and Methods: In this controlled clinical trial, a total of 60 male case with infertility and 20 normospermic fertile men were recruited. Before and after the treatment with DPP, the case were administered 400 mg/kg of gelatinous capsules daily for 30 consecutive days and semen samples were taken. Quantitative real-time polymerase chain reaction was applied for the evaluation of the mRNA expression levels of Nuclear factor erythroid 2-related factor 2(NRF2), superoxide dismutase (SOD2), glutathione peroxidase 4(GPX4), and catalase (CAT) genes. Results: The mRNA expression levels of NRF2, SOD2, GPX4, and CAT (p < 0.05 for all) and significantly increased after treatment with DPP. The increased expressions of all antioxidant genes and enzymes significantly correlated with improvement in semen parameters including count (p = 0.01), motility (p = 0.05), and morphology (p = 0.01) of sperm. A significant correlation between the alteration of SOD2 gene expression and SOD activity, GPX4 and GPX, and CAT were also observed (p = 0.05). Conclusion: DPP can increase the expressions of NRF2, GPX4, SOD2, and CAT genes and also improve the semen quality in infertile men. Key words: DPP, Male infertility, SOD2, NRF2, GPX4, CAT, ROS, Spermatozoa

Corrigendum to: “The effect of Phoenix dactylifera pollen on the expression of NRF2, SOD2, CAT, and GPX4 genes, and sperm parameters of fertile and infertile men: A controlled clinical trial” [Int J Reprod BioMed 2021; 19: 545-558]"

This article is a Corrigendum to: “The effect of Phoenix dactylifera pollen on the expression of NRF2, SOD2, CAT, and GPX4 genes, and sperm parameters of fertile and infertile men: A controlled clinical trial” [Int J Reprod BioMed 2021; 19: 545-558]". Please download the PDF or view the article HTML

Post surgical adhesion prevention: Vitamin C or Satureja Khuzestanica

Abstract    Abdominal surgeries can result in intra abdominal adhesions. Since Satureja Khuzestanica is used for its anti oxidant, anti lipid, anti inflammatory and anti bacterial effects, it might also play a role in preventing intra abdominal adhesion. Therefore, this study was conducted in order to assess the effects of this plant compared with vitamin C in the prevention of post surgical intra abdominal adhesions.Forty rats were used for this experimental study and were randomly allocated into 4 groups of 10 rats. Ten ml of 0.9% normal saline, 250 mg/kg vitamin C, 250 mg/kg Satureja Khuzestanica, and both vitamin C plus Satureja Khuzestanica were used for the first, second, third and fourth group, respectively. Mazuji classification and pathology assessment were used to grade the amount of adhesion. According to our findings, vitamin C in addition to Satureja Khuzestanica is effective in preventing abdominal adhesions

The Effect of Phoenix Dactylifera Pollen on the Expression of NRF2, SOD2, CAT, and GPX4 Genes, and Sperm Parameters of Fertile and Infertile Men: A Controlled Clinical Trial

Background: Oxidative stress is caused by the imbalance occurring between the creation and clearance of the reactive oxygen species (ROS), which is responsible for 30–40% of male infertility. The positive impact of phoenix dactylifera pollen (Date palm pollen, DPP) on the improvement of sperm parameters has been well documented in animal models. Objective: For evaluating the effect(s) of DPP on sperm parameters, ROS levels, expression of antioxidant genes, and activity of antioxidant enzymes of infertile men. Materials and Methods: In this controlled clinical trial, a total of 60 male case with infertility and 20 normospermic fertile men were recruited. Before and after the treatment with DPP, the case were administered 400 mg/kg of gelatinous capsules daily for 30 consecutive days and semen samples were taken. Quantitative real-time polymerase chain reaction was applied for the evaluation of the mRNA expression levels of Nuclear factor erythroid 2-related factor 2(NRF2), superoxide dismutase (SOD2), glutathione peroxidase 4(GPX4), and catalase (CAT) genes. Results: The mRNA expression levels of NRF2, SOD2, GPX4, and CAT (p < 0.05 for all) and significantly increased after treatment with DPP. The increased expressions of all antioxidant genes and enzymes significantly correlated with improvement in semen parameters including count (p = 0.01), motility (p = 0.05), and morphology (p = 0.01) of sperm. A significant correlation between the alteration of SOD2 gene expression and SOD activity, GPX4 and GPX, and CAT were also observed (p = 0.05). Conclusion: DPP can increase the expressions of NRF2, GPX4, SOD2, and CAT genes and also improve the semen quality in infertile men. Key words: DPP, Male infertility, SOD2, NRF2, GPX4, CAT, ROS, Spermatozoa