MicroRNA-222 regulates muscle alternative splicing through Rbm24 during differentiation of skeletal muscle cells

A number of microRNAs have been shown to regulate skeletal muscle development and differentiation. MicroRNA-222 is downregulated during myogenic differentiation and its overexpression leads to alteration of muscle differentiation process and specialized structures. By using RNA-induced silencing complex (RISC) pulldown followed by RNA sequencing, combined with in silico microRNA target prediction, we have identified two new targets of microRNA-222 involved in the regulation of myogenic differentiation, Ahnak and Rbm24. Specifically, the RNA-binding protein Rbm24 is a major regulator of muscle-specific alternative splicing and its downregulation by microRNA-222 results in defective exon inclusion impairing the production of muscle-specific isoforms of Coro6, Fxr1 and NACA transcripts. Reconstitution of normal levels of Rbm24 in cells overexpressing microRNA-222 rescues muscle-specific splicing. In conclusion, we have identified a new function of microRNA-222 leading to alteration of myogenic differentiation at the level of alternative splicing, and we provide evidence that this effect is mediated by Rbm24 protei

Experimental Investigation on Latent Thermal Energy Storages (LTESs) Based on Pure and Copper-Foam-Loaded PCMs

In this work, a commercial paraffin PCM (RT35) characterized by a change range of the solid-liquid phase transition temperature Ts−l=29–36 °C and the low thermal conductivity λSL=0.2 W/m K is experimentally tested by submitting it to thermal charging/discharging cycles. The paraffin is contained in a case with a rectangular base and heated from the top due to electrical resistance. The aim of this research is to show the benefits that a 95% porous copper metal foam (pore density PD=20PPI) can bring to a PCM-based thermal storage system by simply loading it, due to the consequent increase in the effective thermal conductivity of the medium (λLOAD=7.03 W/m K). The experimental results highlight the positive effects of the copper foam presence, such as the heat conduction improvement throughout the system, and a significant reduction in time for the complete melting of the PCM. In addition, the experimental data highlight that in the copper-foam-loaded PCM the maximum temperature reached during the heating process is lower than 20K with respect to the test with pure PCM, imposing the same heat flux on the top (P=3.5 W/m2)

Impact of mild oven cooking treatments on carotenoids and tocopherols of cheddar and depurple cauliflower (Brassica oleracea L. var. Botrytis)

The effect of steam and sous-vide oven procedures on liposoluble antioxidants of colored cauliflower (orange and purple) was assessed for the first time and compared with domestic practice (boiling). In raw samples, the total carotenoid content was 10-fold higher in Cheddar than in Depurple (20.9 \ub1 2.1 vs. 2.3 \ub1 0.5 mg/kg dry weight), whereas the level of tocopherols was similar (28.5 \ub1 4.4 vs. 33 \ub1 5.2 mg/kg dry weight). The Cheddar liposoluble antioxidant matter contained violaxanthin, neoxanthin, \u3b1-carotene and \u3b4-tocopherol, not detected in Depurple. All tests increased the bioactive compounds extractability with steam oven and sous-vide displaying similar effects, lower than boiling. In boiled Cheddar cauliflower, the total carotenoids and tocopherols contents increased with cooking time until they were 13-fold and 6-fold more than in raw cauliflower, respectively. Conversely, in the Depurple variety, contents increased by half with respect to the orange variety. However, from a nutritional point of view, no differences were revealed among the three different cooking treatments in terms of vitamin A and E levels expressed in \u3bcg/100 g of fresh vegetable because of the higher water content of boiled samples that must be considered when evaluating the effect of thermal treatment on cauliflower nutritional traits

The First Survey of X-ray Flares from Gamma Ray Bursts Observed by Swift: Temporal Properties and Morphology

We present the first systematic investigation of the morphological and timing properties of flares in GRBs observed by Swift/XRT. We consider a large sample drawn from all GRBs detected by Swift, INTEGRAL and HETE-2 prior to 2006 Jan 31, which had an XRT follow-up and which showed significant flaring. Our sample of 33 GRBs includes long and short, at low and high redshift, and a total of 69 flares. The strongest flares occur in the early phases, with a clear anti-correlation between the flare peak intensity and the flare time of occurrence. Fitting each X-ray flare with a Gaussian model, we find that the mean ratio of the width and peak time is = 0.13+/-0.10, albeit with a large scatter. Late flares at times > 2000 seconds have long durations, Delta t>300 s, and can be very energetic compared to the underlying continuum. We further investigated if there is a clear link between the number of pulses detected in the prompt phase by BAT and the number of X-ray flares detected by XRT, finding no correlation. However, we find that the distribution of intensity ratios between successive BAT prompt pulses and that between successive XRT flares is the same, an indication of a common origin for gamma-ray pulses and X-ray flares. All evidence indicates that flares are indeed related to the workings of the central engine and, within the standard fireball scenario, originate from internal shocks rather than external shocks. While all flares can be explained by long-lasting engine activity, 29/69 flares may also be explained by refreshed shocks. However, 10 can only be explained by prolonged activity of the central engine.Comment: submitted to Ap

Seesaw model in SO(10) with an upper limit on right-handed neutrino masses

In the framework of SO(10) gauge unification and the seesaw mechanism, we show that the upper bound on the mass of the heaviest right-handed neutrino $M_{R_3} < 3 \times 10^{11}$ GeV, given by the Pati-Salam intermediate scale of $B-L$ spontaneous symmetry breaking, constrains the observables related to the left-handed light neutrino mass matrix. We assume such an upper limit on the masses of right-handed neutrinos and, as a first approximation, a Cabibbo form for the matrix $V^L$ that diagonalizes the Dirac neutrino matrix $m_D$. Using the inverse seesaw formula, we show that our hypotheses imply a triangular relation in the complex plane of the light neutrino masses with the Majorana phases. We obtain normal hierarchy with an absolute scale for the light neutrino spectrum. Two regions are allowed for the lightest neutrino mass $m_1$ and for the Majorana phases, implying predictions for the neutrino mass measured in Tritium decay and for the double beta decay effective mass $||$.Comment: 14 pages. Revised version with 3 figure

Swift X-ray Afterglows and the Missing Jet Break Problem

We present a systematic survey of the temporal and spectral properties of all GRB X-ray afterglows observed by Swift-XRT between January 2005 and July 2007. We have constructed a catalog of all light curves and spectra and investigate the physical origin of each afterglow segment in the framework of the forward shock models by comparing the data with the closure relations. We search for possible jet-like breaks in the lightcurves and try to explain some of the "missing" X-ray jet breaks in the lightcurves.Comment: 4 pages, 4 figures, contributed talk, submitted to the proceedings of Gamma Ray Bursts 2007, Santa Fe, New Mexico, November 5-9 200

Influence of the AOT counterion chemical structure in the generation of organized systems

The impact of the imidazolium counterion structure on the organized systems formed by the surfactant 1,4-bis-2-ethylhexylsulfosuccinate, AOT, both in aqueous solutions and in nonpolar solvents is investigated. With this in mind, we investigated if the ionic liquid-like (IL-like) surfactant 1-ethyl-3-methylimidazolium 1,4-bis-2-ethylhexylsulfosuccinate, emim-AOT, forms direct micelles or vesicles in water. Dynamic light scattering, zeta potential, conductivity, fluorescence spectroscopy, and UV-visible spectroscopy measurements were performed to characterize the organized systems in aqueous solutions. We also studied the self-aggregation of emim-AOT, 1-butyl-3-methylimidazolium 1,4-bis-2-ethylhexylsulfosuccinate, bmim-AOT, and of 1-hexyl-3-methylimidazolium 1,4-bis-2-ethylhexylsulfosuccinate, hmim-AOT, in nonpolar solvents. The results obtained showed that the IL-like surfactant emim-AOT forms direct micelles in water, as sodium 1,4-bis-2-ethylhexylsulfosuccinate (Na-AOT) does. However, emim-AOT aggregates are larger, have a lower surface charge, are more stable, and have a more polar and less fluid micellar interface than Na-AOT micelles. It was also observed that emim-AOT and hmim-AOT form reverse micelles in nonpolar solvents. The size of the imidazolium cations dramatically influences the size of the reverse micelles and their ability to solubilize water.Fil: Lépori, Cristian Marcelo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina. Universidad Nacional de Córdoba; ArgentinaFil: Correa, Nestor Mariano. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Silber, Juana J.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Falcone, Ruben Dario. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: López-López, Manuel. Universidad de Huelva; EspañaFil: Moyá, María Luisa. Universidad de Sevilla; Españ

X-ray flares from propagation instabilities in long Gamma-Ray Burst jets

We present a numerical simulation of a gamma-ray burst jet from a long-lasting engine in the core of a 16 solar mass Wolf-Rayet star. The engine is kept active for 6000 s with a luminosity that decays in time as a power-law with index -5/3. Even though there is no short time-scale variability in the injected engine luminosity, we find that the jet's kinetic luminosity outside the progenitor star is characterized by fluctuations with relatively short time scale. We analyze the temporal characteristics of those fluctuations and we find that they are consistent with the properties of observed flares in X-ray afterglows. The peak to continuum flux ratio of the flares in the simulation is consistent with some, but not all, the observed flares. We propose that propagation instabilities, rather than variability in the engine luminosity, are responsible for the X-ray flares with moderate contrast. Strong flares such as the one detected in GRB 050502B, instead, cannot be reproduced by this model and require strong variability in the engine activity.Comment: 6 pages, MNRAS in pres