Evaluation of peri-implant soft and hard tissues around dental implants placed at the Faculty of Dentistry, UiTM / Mohd Faizal Hafez Hidayat … [et al.]

This study is carried out to assess the soft and hard tissue conditions around dental implant cases at the Faculty of Dentistry Universiti Teknologi MARA (UiTM). Methods: Patients with dental implants of more than 6 months in function were recalled for clinical and radiographic evaluation. Probing pocket depth, suppuration, bleeding, recession and plaque index were clinically evaluated. Periapical radiograph (IOPA) were also taken. The data from the findings were statistically evaluated using SPSS program. Result: 14.3% of implants placed in consider healthy, 4.8% implants have clinical stability and 81% have peri-implant mucositis and none of the participants have peri-implantitis and severe peri-implantitis. Conclusion: Within the limitation of this study, it’s concluded that the status of peri-implant tissues is mainly affected by gender, systemic disease, smoking status,oral hygiene and years of implants functioning in oral condition. In addition, age plays a significant role that contribute to periimplant health

Salivary transcriptome biomarkers: For the identification of periodontitis susceptibility.

Periodontitis (gum disease) is a chronic infectious disease affecting the supporting tissue around the teeth. Bacteria cause the infection and subsequently activate the natural inflammatory host response. The response to bacterial infection varies between individuals. Epidemiological studies have shown that only a minority of the population are susceptible to advanced chronic periodontitis while the majority of the population have mild to moderate forms of the disease. Identifying individuals who are susceptible to periodontitis will enable focused patient management and timely preventive programs. A readily available and non-invasive source of potential biomarkers is saliva. The “salivary transcriptome” defines the RNA present in saliva. Significant changes to the salivary transcriptome of oral cancer patients have been described previously. The aim of this study was to discover potential biomarkers of susceptibility with the identification of mRNAs that are differentially expressed in the saliva of healthy and chronic periodontitis patients. Using an Oragene® RNA kit total RNA was purified from the saliva of 10 chronic periodontitis patients and 10 with healthy or only mildly inflamed gingivae (the health/gingivitis group). The quantity and quality of the total RNA was determined, and a measure of gene expression via cDNA was undertaken using the Affymetrix Microarray system. The microarray profiling result was further validated by real-time quantitative PCR. The result showed that there was acceptable quality and quantity of total RNA from saliva but a high proportion of it was of microbial origin and there was insufficient human salivary transcriptome for expression studies. Detecting the human salivary transcriptome is difficult as it is mostly partially fragmented and degraded in saliva. Nevertheless, the prospect of identifying a saliva biomarker in the gene expression profile of susceptible patients is novel however, further work is required to enhance the extraction process of human mRNA from saliva

Salivary transcriptome biomarkers: For the identification of periodontitis susceptibility.

Periodontitis (gum disease) is a chronic infectious disease affecting the supporting tissue around the teeth. Bacteria cause the infection and subsequently activate the natural inflammatory host response. The response to bacterial infection varies between individuals. Epidemiological studies have shown that only a minority of the population are susceptible to advanced chronic periodontitis while the majority of the population have mild to moderate forms of the disease. Identifying individuals who are susceptible to periodontitis will enable focused patient management and timely preventive programs. A readily available and non-invasive source of potential biomarkers is saliva. The “salivary transcriptome” defines the RNA present in saliva. Significant changes to the salivary transcriptome of oral cancer patients have been described previously. The aim of this study was to discover potential biomarkers of susceptibility with the identification of mRNAs that are differentially expressed in the saliva of healthy and chronic periodontitis patients. Using an Oragene® RNA kit total RNA was purified from the saliva of 10 chronic periodontitis patients and 10 with healthy or only mildly inflamed gingivae (the health/gingivitis group). The quantity and quality of the total RNA was determined, and a measure of gene expression via cDNA was undertaken using the Affymetrix Microarray system. The microarray profiling result was further validated by real-time quantitative PCR. The result showed that there was acceptable quality and quantity of total RNA from saliva but a high proportion of it was of microbial origin and there was insufficient human salivary transcriptome for expression studies. Detecting the human salivary transcriptome is difficult as it is mostly partially fragmented and degraded in saliva. Nevertheless, the prospect of identifying a saliva biomarker in the gene expression profile of susceptible patients is novel however, further work is required to enhance the extraction process of human mRNA from saliva

The impact of vitamin D on clinical parameters and bone turnover biomarkers in ligature-induced periodontitis: An experimental study in rats

Objectives and Background: Vitamin D has been associated with an increased risk of tooth loss and the severity of periodontal diseases. This study aimed to evaluate the effect of vitamin D on the clinical, radiographic, and serum level changes of bone turnover biomarkers in ligature-induced periodontitis. Methods: A total of 28 rats were included in this study and divided into test groups: Vitamin D supplement (VS), Vitamin D deficient (VD), and control (CG). Ligature-induced periodontal tissue destruction was performed and kept for 21 days. Clinical attachment and radiographic changes were recorded, and serum samples were tested for Osteoprotegerin (OPG), Dickkopf-1 (DKK1), Sclerostin (SOST), and Fibroblast growth factor 23 (FGF23) on the initial and final day of the study. Results: Groups that were made VD exhibited a more significant amount of clinical attachment loss (1.05 ± 0.50 mm) compared to the CG (0.83 ± 0.14 mm) and VS group (0.60 ± 0.13 mm), showing significant differences (p < 0.05). The radiographic alveolar bone loss amount was greater in the VD group compared to the other groups. For serum level assessment, the VD groups also exhibited a statistically significant reduction in the levels of OPG. They showed higher concentrations of DKK1, SOST, and FGF23 than other groups, with significant differences (p < 0.05). Conclusion: The results revealed that Vitamin D may play a role in the progression of periodontal disease. It was found to affect both clinical parameters and bone turnover biomarkers, suggesting its potential impact on the disease process

Interdisciplinary approach involving medical colleagues in managing periodontitis patient with type 2 diabetes: a case report

Introduction: Diabetes mellitus has been recognized as a systemic risk factor for periodontitis. Success in controlling periodontitis requires interdisciplinary solutions involving medical counterparts. Case report: This case report highlights the importance of a bidirectional communication between medical and periodontal specialist in the management of a 63-year-old Malay lady patient diagnosed with Generalized Periodontitis, Stage IV and Grade C. The outcome of the interdisciplinary approach was both the stabilization of her periodontal conditions and medically, the glycated haemoglobin level. Conclusions: The bidirectional communication between medical and periodontal specialist is as important as managing the patients medically for a holistic treatment approach of an uncontrolled diabetic patient