CAIMAN Experiment

Non-acoustic detection systems can be used in combination with Sonar systems to determine the presence of an underwater threat, such as terrorist divers. The goal of the CAIMAN (Coastal Anti Intruders MAgnetometers Network) joint experiment (Italian Navy, NATO Undersea Research Centre and INGV Marine Geophysics) is the application of High Definition Geophysics Magnetic techniques in a port protection scenario, where conventional measurements of very low magnetic sources, like intruder swimmers, are strongly disturbed by ambient, natural and artificial, background noise and other time-variant magnetic anomalies. Two tri-axial fluxgate magnetometers were deployed on the sea bottom and connected to a shore side measurement station. A team of navy divers, wearing both COTS and EOD equipment, performed some coastal approach runs on each magnetometer alternatively. Magnetic signature data were logged and post processed using MATLAB®. Results demonstrated the effectiveness of high definition time reduction techniques using a self-referred integrated array design

Chloroquine supplementation increases the cytotoxic effect of curcumin against Her2/neu overexpressing breast cancer cells in vitro and in vivo in nude mice while counteracts it in immune competent mice

Autophagy is usually a pro-survival mechanism in cancer cells, especially in the course of chemotherapy, thus autophagy inhibition may enhance the chemotherapy-mediated anti-cancer effect. However, since autophagy is strongly involved in the immunogenicity of cell death by promoting ATP release, its inhibition may reduce the immune response against tumors, negatively influencing the overall outcome of chemotherapy. In this study, we evaluated the in vitro and in vivo anti-cancer effect of curcumin (CUR) against Her2/neu overexpressing breast cancer cells (TUBO) in the presence or in the absence of the autophagy inhibitor chloroquine (CQ). We found that TUBO cell death induced by CUR was increased in vitro by CQ and slightly in vivo in nude mice. Conversely, CQ counteracted the Cur cytotoxic effect in immune competent mice, as demonstrated by the lack of in vivo tumor regression and the reduction of overall mice survival as compared with CUR-treated mice. Immunohistochemistry analysis revealed the presence of a remarkable FoxP3 T cell infiltrate within the tumors in CUR/CQ treated mice and a reduction of T cytotoxic cells, as compared with single CUR treatment. These findings suggest that autophagy is important to elicit anti-tumor immune response and that autophagy inhibition by CQ reduces such response also by recruiting T regulatory (Treg) cells in the tumor microenvironment that may be pro-tumorigenic and might counteract CUR-mediated anti-cancer effects

A phase 1 study evaluating the pharmacokinetics, safety and tolerability of repeat dosing with a human IL-13 antibody (CAT-354) in subjects with asthma

<p>Abstract</p> <p>Background</p> <p>IL-13 has been implicated in the development of airway inflammation and hyperresponsiveness. This study investigated the multiple-dose pharmacokinetics and safety profile of human anti-IL-13 antibody (CAT-354) in adults with asthma.</p> <p>Methods</p> <p>This was a multiple-dose, randomised, double-blind, placebo-controlled phase 1 study in asthmatics (forced expiratory volume in 1 second [FEV₁] ≥ 80% predicted). Subjects were randomised to receive three intravenous infusions of CAT-354 (1 mg/kg, 5 mg/kg or 10 mg/kg) or placebo at 28-day intervals. Blood samples were taken for pharmacokinetic measurements. Safety was assessed by adverse events, vital signs, ECGs, laboratory and pulmonary function parameters.</p> <p>Results</p> <p>Twenty-three subjects (aged 21-60 years, FEV₁88-95% predicted) received ≥ 1 dose of study medication. The half-life of CAT-354 was 12-17 days and was dose-independent. The maximum serum concentration and area under the curve were dose-dependent. Clearance (2.2-2.6 mL/day/kg) and volume of distribution (44-57 mL/kg) were both low and dose-independent. The observed maximum serum concentration after each dose increased slightly from dose 1 through dose 3 at all dose levels, consistent with an accumulation ratio of 1.4 to 1.7 for area under the curve. Most adverse events were deemed mild to moderate and unrelated to study medication. One SAE was reported and deemed unrelated to study drug. There were no effects of clinical concern for vital signs, ECG, laboratory or pulmonary parameters.</p> <p>Conclusions</p> <p>CAT-354 exhibited linear pharmacokinetics and an acceptable safety profile. These findings suggest that at the doses tested, CAT-354 can be safely administered in multiple doses to patients with asthma.</p> <p>Trial registration</p> <p>NCT00974675.</p

Anti-intruder port protection “MAC (Magnetic ACoustic) System”: advances in the magnetic component performance

In the following, we report the advances made in the development of a magnetic component for peripheral monitoring of port environments for anti-intruder systems. The project was initiated by the Office of Research and Development, COMFORDRAG, Italian Navy, in 2004, with the aim of improving the detection performance of current acoustic-based anti-intruder systems, in the vicinity of quays or the seabed or in acoustic-shadow zones behind natural or artificial objects. In the five-year period from 2004 to 2008, the system has been subjected to detection-performance tests in experiments involving divers equipped with air tanks and rebreather kits in real port environments and, at the same time, the associated signal processing has been subjected to development, to enhance detection performance. In tests involving simulated diver attacks against a vessel moored alongside a quay in the port of La Spezia, the system has been proved particularly effective by detecting 9 divers out of 9. The processing of the magnetic signal has been enhanced by implementing an amplitude threshold, as a means of filtering the passive energy components, termed Passive Energy Cutting (PEC), that is, the elimination of components that comprise no useful information regarding diver transits. The use of PEC boosts the reliability of the system in terms of detection versus false alarm rate, as evidenced by the comparison of the ROC curves for the system with and without PEC

Viral Infection and Autophagy Dysregulation. The Case of HHV-6, EBV and KSHV

Human Herpes Virus-6 (HHV-6), Epstein-Barr Virus (EBV) and Kaposi Sarcoma Herpes Virus (KSHV) are viruses that share with other member of the Herpesvirus family the capacity to interfere with the autophagic process. In this paper, mainly based on the findings of our laboratory, we describe how, through different mechanisms, these viruses converge in reducing autophagy to impair DC immune function and how, by infecting and dysregulating autophagy in different cell types, they promote the pathologies associated with their infection, from the neurodegenerative diseases such Alzheimer's disease to cancer

SCAI-r: an algorithm for obtaining synoptic and spatial atmospheric parameters.

Abstract: The highest performance of multiband optical data (Visible - VIS ? Shortwave Infrared - SWIR ? (400-2500 nm) (e.g. imaging spectroscopy approaches) can be reached mainly through atmospheric correction effects; the conversion of the image values to surface reflectance, allowing, for instance, to estimate the amount of biochemical compounds in the target. This work aims to present an algorithm dedicated to atmospheric parameters obtaining from MODIS and related sensors, encapsulated in a user-friendly interface through Software to Collect Atmospheric Information (SCAI-r), applied to the correction of atmosphere effects on Rapideye image. The results show that SCAI-r generates information needed to perform atmospheric correction and that it has a greater influence over water-related objects, when compared to parameters randomly inserted as input data in atmospheric correction algorithms

Targeting COX-2/PGE(2) pathway in HIPK2 knockdown cancer cells: impact on dendritic cell maturation.

production after HIPK2 depletion and how to modulate it./VEGF production. At translational level, while conditioned media of both siRNA control and HIPK2 depleted cells inhibited DCs maturation, conditioned media of only zinc-treated HIPK2 depleted cells efficiently restored DCs maturation, seen as the expression of co-stimulatory molecules CD80 and CD86, cytokine IL-10 release, and STAT3 phosphorylation./VEGF production; and 3) zinc treatment of HIPK2 depleted cells restored DCs maturation

Harbour water managment for port structures and sea bottom design, coast proximity navigation managment, water quality control.

Tide is a sea level up-down water motion basically depending on three different phenomena: the Earth-Moon-Sun gravitational relationship, the water surface fluid reaction to atmospheric meteorological dynamic action and the Newtonian vertical adjustment of the sea surface due to atmospheric pressure variations. The first tide component (astro-tide) is periodic and well known in all points of the Earth surface; the second one is directly related to the meteorological phenomenon and then it is foreseeable; the Newtonian component, on the contrary, is not readily predictable by a general hydrostatic law, because the factor “J” that represents the Newtonian transfer (from the atmospheric weight to the consequent sea level) is variable in each harbour area. A statistical study and the related numerical data interpretation of the measurements performed in the Ports of Genoa, La Spezia, Marina di Carrara, Livorno, Piombino, Civitavecchia and Ravenna (belonging to the Italian Newtonian Meteotide Network) show port values of Jph (from 1.4-1.6 cm/hPa to > 2 cm/hPa, on the contrary of the off-shore areas where Jph is about 1 cm/hPa). This phenomenon (hydrobarometric tide wave) produces even double values of harbour sea level fluctuations amplitude in comparison to astronomic tide sea level oscillations, and is characterized by a wavelength from 8-12 h to same days and a 10-25 days/year as mean temporal occurrence in the Northern Tyrrhenian Sea. This is the most relevant ordinary risk factor for our ports activity and structures design. The present note shows a quantitative method to define the values of Jph factor for ports and its use in the Harbour WaterSide Management (HWSM) based on the joined use of barometers, hydrometers and clocks, the preliminary results related to the use of the gravimeters as hydrobarometric predictor in La Spezia Port and two examples of use of Jph factor in the port management: refloating of a landing ship and optimisation of a dock performance as pleasure boats mooring

Multislice computed tomography SYNTAX score for coronary artery disease evaluation prior to transcatheter aortic valve implantation

Background: Coronary computed tomography angiography (CCTA) is a useful tool for the evaluation of coronary anatomy prior to both surgical and transcatheter aortic valve implantation (TAVI). Multislice Computed Tomography (MSCT) SYNTAX score (SXscore) strongly correlates with the traditional angiographic SXscore, and the latter has proven to predict cardiovascular events in patients with coronary artery disease (CAD) referred to TAVI. Purpose: The aim of the study is to evaluate the feasibility and accuracy of the calculation of MSCT SXscore in TAVI patients, compared to the gold standard angiographic SXscore. Materials and methods: We evaluated 65 patients eligible for TAVI who underwent both CCTA and invasive coronary angiography (ICA) prior to valve replacement. CCTA was compared to ICA in terms of sensitivity, specificity, and positive and negative predictive values. CCTA performance was evaluated at 3 levels: patient level, vessellevel and segmentlevel. MSCT SXscore was calculated, when possible (i.e. only in fullyevaluable scans), and compared to the angiographic SXscore. Results: Overall CCTA diagnostic performance was good, with high sensitivity and negative predictive values (97.2% and 96.0%, respectively) and good agreement with ICA (k=0.81). As expected, specificity and positive predictive values were lower (82.8% and 87.5%, respectively). At vessellevel, the circumflex artery (CA) was more often misdiagnosed than the other arteries. We were able to calculate MSCT SXscore in 50/65 scans (76.9%). The correlation between MSCT and angiographic SXscore was excellent (Pearson's R=0.965, P&lt;0.001). Conclusions: MSCT SXscore emerges as an interesting tool with strong agreement with angiographic SXscore, providing a noninvasive ambulatory alternative to assess CAD severity in TAVI patients