Genetic study of the production of sexually dimorphic cuticular hydrocarbons in relation with the sex-determination gene transformer in Drosophila melanogaster

0016-6723 (Print) Journal Article Research Support, Non-U.S. Gov'tIn Drosophila melanogaster, the main cuticular hydrocarbons (HCs) are some of the pheromones involved in mate discrimination. These are sexually dimorphic in both their occurrence and their effects. The production of predominant HCs has been measured in male and female progeny of 220 PGa14 lines mated with the feminising UAS-transformer transgenic strain. In 45 lines, XY flies were substantially or totally feminised for their HCs. Surprisingly, XX flies of 14 strains were partially masculinised. Several of the PGa14 enhancer-trap variants screened here seem to interact with sex determination mechanisms involved in the control of sexually dimorphic characters. We also found a good relationship between the degree of HC transformation and GAL4 expression in oenocytes. The fat body was also involved in the switch of sexually dimorphic cuticular hydrocarbons but its effect was different between the sexes

Temperature affects the ontogeny of sexually dimorphic cuticular hydrocarbons in Drosophila melanogaster

0022-0949 (Print) Journal Article Research Support, Non-U.S. Gov'tHydrocarbons on the cuticle of mature Drosophila melanogaster flies play a crucial role in mate recognition, and protect against dehydration. We measured the effect of temperature on mature cuticular hydrocarbons (CHs) by (i) rearing two control strains at different temperatures, (ii) shifting the temperature after metamorphosis and (iii) inducing a single heat-shock pulse in control and heat-sensitive transgenic strains, over a period of 3 days following adult eclosion. This study describes the time course of the events involved in the production of male- and female-predominant CHs. We also found that 'immature' CHs, sexually monomorphic CHs on younger flies, were not affected by these treatments

A Mutation With Major Effects on Drosophila melanogaster Sex Pheromones

Sex pheromones are intraspecific chemical signals that are crucial for mate attraction and discrimination. In Drosophila melanogaster, the predominant hydrocarbons on the cuticle of mature female and male flies are radically different and tend to stimulate or inhibit male courtship, respectively. This sexual difference depends largely upon the number of double bonds (one in males and two in females) added by desaturase enzymes. A mutation was caused by a PGal4 transposon inserted in the desat1 gene that codes for the desaturase crucial for setting these double bonds. Homozygous mutant flies produced 70–90% fewer sex pheromones than control flies, and the pheromonal difference between the sexes was almost abolished. A total of 134 excision alleles were induced by pulling out all or a part of the transposon. The pheromonal profile was generally rescued in excision alleles with a completely or largely removed transposon whereas it remained mutant in alleles with a larger piece of the transposon. Five desat1 transcripts were detected during larval-to-adult development. Their levels were precisely quantified in 24-hr-old adults, a critical period for the production of sex pheromones. Three transcripts significantly varied between control females and males; however, the predominant transcript showed no difference. In mutant flies, the predominant transcript was highly decreased with the two sexually dimorphic transcripts.These two transcripts were also absent in the sibling species D. simulans, which shows no sexually dimorphic hydrocarbons. We also induced a larval-lethal allele that lacked all transcripts and failed to complement the defective hydrocarbon phenotype of mutant alleles

Does the queen win it all? Queen-worker conflict over male production in the bumblebee, Bombus terrestris

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Genetic elimination of known pheromones reveals the fundamental chemical bases of mating and isolation in Drosophila

Overexpression of the UAS-tra transgene in Drosophila melanogaster females led to the complete elimination of their cuticular pheromones. According to current models of Drosophila behavior, these flies should induce no courtship. In fact, they are still attractive to conspecific males. Three classes of stimuli are shown to induce courtship, with different effects on male behavior: (i) known pheromones produced by control females, (ii) stimuli produced by living control and transgenic flies, and (iii) as-yet-undetermined pheromones present on both control and transgenic flies. Only the latter class of pheromones are required for mating. They appear to represent a layer of ancestral attractive substances present in D. melanogaster and its sibling species; known cuticular pheromones modulate this attractivity positively or negatively. The absence of inhibitory pheromones leads to high levels of interspecific mating, suggesting an important role for these cuticular hydrocarbons in isolation between species

Age-specific olfactory attraction between Western honey bee drones (Apis mellifera) and its chemical basis.

During the mating season, drones (males) of the Western honey bee (Apis mellifera) form congregations numbering thousands high in the air. Virgin queens arrive at these congregations after they have formed and mate on the fly with 15-20 drones. To explain the formation of drone congregations, a drone-produced aggregation pheromone has been proposed many years ago but due to the low accessibility of natural mating sites in bees, its study has progressed slowly. Recently, we used a walking simulator in controlled laboratory conditions to show that drones are indeed attracted by groups of other drones. Since these previous experiments were carried out with drones captured when flying out of the hive, it is currently unclear if this olfactory attraction behaviour is related to the drones' sexual maturity (usually reached between 9 and 12 days) and may thus be indicative of a possible role in congregation formation, or if it is observed at any age and may represent in-hive aggregation. We thus assessed here the dependency of drone olfactory attraction on their age. First, we performed behavioural experiments in the walking simulator to measure olfactory preferences of drones in three age groups from 2-3 to 12-15 days. Then, we performed chemical analyses in the same age groups to evaluate whether chemical substances produced by the drones may explain age differences in olfactory attraction. We show that honey bee drones are attracted by conspecifics of the same age when they are sexually mature (12-15 days old) but not when they are younger (2-3 and 7-8 days old). In parallel, our data show that drones' chemical profile changes with age, including its most volatile fraction. These results are discussed in the context of drone mutual attraction both within the hive and at drone congregations

Discrimination of the Social Parasite Ectatomma parasiticum by Its Host Sibling Species (E. tuberculatum)

Among social parasites, workerless inquilines entirely depend on their host for survival and reproduction. They are usually close phylogenetic relatives of their host, which raises important questions about their evolutionary history and mechanisms of speciation at play. Here we present new findings on Ectatomma parasiticum, the only inquiline ant described in the Ectatomminae subfamily. Field data confirmed its rarity and local distribution in a facultative polygynous population of E. tuberculatum in Mexico. Genetic analyses demonstrated that the parasite is a sibling species of its host, from which it may have diverged recently. Polygyny is suggested to have favored the evolution of social parasite by sympatric speciation. Nevertheless, host workers from this population were able to discriminate parasites from their conspecifics. They treated the parasitic queens either as individuals of interest or as intruders, depending on their colonial origin, probably because of the peculiar chemical profile of the parasites and/or their reproductive status. We suggest that E. parasiticum could have conserved from its host sibling species the queen-specific substances that produce attracting and settling effect on workers, which, in return, would increase the probability to be detected. This hypothesis could explain the imperfect social integration of the parasite into host colonies