Genetic, ecological, behavioral and geographic differentiation of populations in a thistle weevil: implications for speciation and biocontrol

Because weevils are used as biocontrol agents against thistles, it is important to document and understand host shifts and the evolution of host-specificity in these insects. Furthermore, such host shifts are of fundamental interest to mechanisms of speciation. The mediterranean weevil Larinus cynarae normally parasitizes either one of two thistle genera, Onopordum and Cynara, being locally monophagous. In Sardinia, however, both host genera are used. We used three types of data to help understand this complex host use: (i) weevil attack rates on the two host genera among 53 different populations in Sardinia and nearby Corsica, (ii) host preference in a lab setting, and (iii) genetic (allozyme) differentiation among weevil populations exploiting the same or different hosts. Using a subset of populations from northern Sardinia, we attempted to relate interpopulation differences in host preference to gene flow among populations by comparing pairwise differences in oviposition preference (Qst) and in allozyme frequencies (Fst). Overall, Qst and Fst were positively correlated. Fst was positively correlated with geographic distance among pairs of populations using the same host, but not among different-host population pairs. As mating occurs on the hosts, this result suggests reinforcement. Genetic evidence indicates Cynara as the ancestral host of the weevils from both islands and our current studies suggest repeated attempts to colonize Onopordum, with a successful shift in Corsica and a partial shift in Sardinia. This scenario would explain why in Sardinia the level of attack was higher on Cynara than on Onopordum and why, when given a choice in the laboratory, Sardinian weevils preferred Cynara even when sampled from Onopordum. The lability of host shifts in L. cynarae supports caution in using these or related weevils as biocontrol agents of exotic thistles

Insights from 180 years of mitochondrial variability in the endangered Mediterranean monk seal (Monachus monachus)

Mediterranean monk seals (MMS) are among the most endangered marine mammals on Earth.We screened mitochondrial variability (control region [CR1] and mitogenomes) of the species through a 180‐yr timeframe and extended by 20% (n = 205) the number of samples from a previous investigation, including historical specimens from 1833 to 1975. Although we detected two new, rare CR1 haplotypes, genetic diversity remained extremely low. Fully resolved haplotype median network and rarefaction analysis both suggested low probability for further unscreened haplotypes. There was no clear phylogeographic structure across the 12 marine subdivisions covered by the species' range. Haplotypes previously considered diagnostic of the extant North Atlantic and eastern Mediterranean populations had their distributions extended into the western Mediterranean and the North Atlantic, respectively, by both historical and recent samples. Our study suggests that MMS have been genetically depauperate since at least the mid‐19th century, and that the massive 1997 die‐off in Western Sahara (North Atlantic) could have caused local haplotype extinctions. Our results support the hypothesis of past metapopulation dynamics across the species range, where the current segregation into geographically distant and genetically depauperate breeding populations (i.e., North Atlantic and eastern Mediterranean Sea) derives from the combined effects of historical extinctions, genetic drift on small breeding groups, and persistently low levels of genetic diversity

Comparative allozyme and microsatellite population structure in a narrow endemic plant species, Centaurea corymbosa Pourret (Asteraceae)

International audienceCentaurea corymbosa Pourret (Asteraceae) is a narrow endemic species known only from six populations located in a 3-km2 area in the south of France. Earlier field experiments have suggested that pollen and seed dispersal were highly restricted within and among populations. Consistent with the field results, populations were highly differentiated for five allozyme loci and among-population variation fitted an isolation-by-distance model. In the present study, we investigated the genetic structure of C. corymbosa using six microsatellite loci. As with allozymes, microsatellites revealed no within-population structure and a large differentiation among populations. However, allozyme loci were less powerful than microsatellites in detecting the extent of gene flow assessed by assignment tests. The patterns of structuration greatly varied among loci for both types of marker; we suggest that differences in single-locus pattern could mainly be an effect of stochastic variation for allozymes and an effect of variation in mutation rate for microsatellites. In contrast to the multilocus results, the two most polymorphic microsatellite loci did not show any isolation-by-distance pattern. Our results suggest that highly variable loci might not always be the best suited markers to quantify levels of gene flow among populations

Isolation of ten microsatellite markers using a pyrosequencing procedure and cross-priming in the Salaria genus

International audienceTen new microsatellite markers were isolatedfrom the freshwater blenny, Salaria fluviatilis. These lociwere characterised for three distinct populations (VarRiver and Lake Bourget in France, and Lake Kinneret inIsrael). S. fluviatilis populations in the North-West of theMediterranean Sea are all polymorphic and under Hardy–Weinberg equilibrium. These microsatellite markers aresuggested for studying the genetic structure in this area.Seven of the ten microsatellite markers are applicable forall the freshwater blenny populations of the entire Mediterraneanbasin. Cross-species characterisation was alsoperformed on the peacock blenny, Salaria pavo. Seven ofthe ten microsatellite loci exhibited successful PCRamplification on this latter species. Polymorphism isobserved on four of these seven loci

Isolation and characterization of microsatellites in the endemic species Centaurea corymbosa Pourret (Asteraceae) and other related species

PRIMER NOTEInternational audienc

The cellular phenotype of cytoplasmic incompatibility in Culex pipiens in the light of cidB diversity.

Wolbachia are maternally inherited endosymbiotic bacteria, widespread among arthropods thanks to host reproductive manipulations that increase their prevalence into host populations. The most commonly observed manipulation is cytoplasmic incompatibility (CI). CI leads to embryonic death in crosses between i) infected males and uninfected females and ii) individuals infected with incompatible Wolbachia strains. CI can be conceptualized as a toxin-antidote system where a toxin deposited by Wolbachia in the sperm would induce embryonic death unless countered by an antidote produced by Wolbachia present in the eggs. In Drosophila melanogaster, transgenic expression of Wolbachia effector cidB revealed its function of CI-inducing toxin. Moreover in Culex pipiens, the diversity of cidB variants present in wPip strains accounts for the diversity in crossing-types. We conducted cytological analyses to determine the CI mechanisms that lead to embryonic death in C. pipiens, and assess whether diversity in crossing-types could be based on variations in these mechanisms. We revealed that paternal chromatin condensation and segregation defects during the first embryonic division are always responsible for embryonic death. The strongest observed defects lead to an exclusion of the paternal chromatin from the first zygotic division, resulting in haploid embryos unable to hatch. The proportion of unhatched haploid embryos, developing with only maternal chromatin, which reflects the frequency of strong defects can be considered as a proxy of CI intensity at the cellular level. We thus studied the putative effect of variations in crossing types and cidB diversification on CI defects intensity. Incompatible crosses involving distinct wPip strains revealed that CI defects intensity depends on the Wolbachia strains hosted by the males and is linked to the diversity of cidB genes harbored in their genomes. These results support that, additionally to its implication in C. pipiens crossing type variability, cidB diversification also influences the strength of CI embryonic defects

Discovery of Culex pipiens associated tunisia virus: a new ssRNA(+) virus representing a new insect associated virus family

International audienceIn the global context of arboviral emergence, deep sequencing unlocks the discovery of new mosquito-borne viruses. Mosquitoes of the species Culex pipiens, C. torrentium, and C. hortensis were sampled from 22 locations worldwide for transcriptomic analyses. A virus discovery pipeline was used to analyze the dataset of 0.7 billion reads comprising 22 individual transcriptomes. Two closely related 6.8 kb viral genomes were identified in C. pipiens and named as Culex pipiens associated tunisia virus (CpATV) strains Ayed and Jedaida. The CpATV genome contained four ORFs. ORF1 possessed helicase and RNA-dependent RNA polymerase (RdRp) domains related to new viral sequences recently found mainly in dipterans. ORF2 and 4 contained a capsid protein domain showing strong homology with Virgaviridae plant viruses. ORF3 displayed similarities with eukaryotic Rhoptry domain and a merozoite surface protein (MSP7) domain only found in mosquito-transmitted Plasmodium, suggesting possible interactions between CpATV and vertebrate cells. Estimation of a strong purifying selection exerted on each ORFs and the presence of a polymorphism maintained in the coding region of ORF3 suggested that both CpATV sequences are genuine functional viruses. CpATV is part of an entirely new and highly diversified group of viruses recently found in insects, and that bears the genomic hallmarks of a new viral family

Characterization and Multiplexing of 21 Microsatellite Markers for the Herb Noccaea caerulescens (Brassicaceae)

Premise of the study: Multiplexed microsatellite markers were developed for population genetic studies in the pseudometallophyteNoccaea caerulescens (Brassicaceae), a model species to investigate metal tolerance and hyperaccumulation in higherplants.• Methods and Results: Microsatellite loci were isolated through pyrosequencing of an enriched DNA library. Three multiplexescombining four previously published and 17 newly designed markers were developed. The new markers were screened inmetallicolous and nonmetallicolous populations from southern France. The total number of alleles per locus ranged from fi veto 18. The observed heterozygosity per locus and per population ranged from 0 to 0.83, and expected heterozygosity rangedfrom 0 to 0.89.• Conclusions: The investigated loci showed reasonable to high levels of polymorphism at the regional scale. The multiplex setshould be helpful in investigating genetic diversity, population structure, and demographic history in N. caerulescens at variousspatial scales

In Cold Blood: Compositional Bias and Positive Selection Drive the High Evolutionary Rate of Vampire Bats Mitochondrial Genomes

International audienceMitochondrial genomes of animals have long been considered to evolve under the action of purifying selection. Nevertheless, there is increasing evidence that they can also undergo episodes of positive selection in response to shifts in physiological or environmental demands. Vampire bats experienced such a shift, as they are the only mammals feeding exclusively on blood and possessing anatomical adaptations to deal with the associated physiological requirements (e.g., ingestion of high amounts of liquid water and iron). We sequenced eight new chiropteran mitogenomes including two species of vampire bats, five representatives of other lineages of phyllostomids and one close outgroup. Conducting detailed comparative mitogenomic analyses, we found evidence for accelerated evolutionary rates at the nucleotide and amino acid levels in vampires. Moreover, the mitogenomes of vampire bats are characterized by an increased cytosine (C) content mirrored by a decrease in thymine (T) compared with other chiropterans. Proteins encoded by the vampire bat mitogenomes also exhibit a significant increase in threonine (Thr) and slight reductions in frequency of the hydrophobic residues isoleucine (Ile), valine (Val), methionine (Met), and phenylalanine (Phe). We show that these peculiar substitution patterns can be explained by the co-occurrence of both neutral (mutational bias) and adaptive (positive selection) processes. We propose that vampire bat mitogenomes may have been impacted by selection on mitochondrial proteins to accommodate the metabolism and nutritional qualities of blood meals