34 research outputs found
Surgical treatment of chronic acromioclavicular joint dislocation with autogenous tendon grafts
Vertebrate Vitellogenin Gene Duplication in Relation to the â3R Hypothesisâ: Correlation to the Pelagic Egg and the Oceanic Radiation of Teleosts
The spiny ray-finned teleost fishes (Acanthomorpha) are the most successful group of vertebrates in terms of species diversity. Their meteoric radiation and speciation in the oceans during the late Cretaceous and Eocene epoch is unprecedented in vertebrate history, occurring in one third of the time for similar diversity to appear in the birds and mammals. The success of marine teleosts is even more remarkable considering their long freshwater ancestry, since it implies solving major physiological challenges when freely broadcasting their eggs in the hyper-osmotic conditions of seawater. Most extant marine teleosts spawn highly hydrated pelagic eggs, due to differential proteolysis of vitellogenin (Vtg)-derived yolk proteins. The maturational degradation of Vtg involves depolymerization of mainly the lipovitellin heavy chain (LvH) of one form of Vtg to generate a large pool of free amino acids (FAA 150â200 mM). This organic osmolyte pool drives hydration of the ooctye while still protected within the maternal ovary. In the present contribution, we have used Bayesian analysis to examine the evolution of vertebrate Vtg genes in relation to the â3R hypothesisâ of whole genome duplication (WGD) and the functional end points of LvH degradation during oocyte maturation. We find that teleost Vtgs have experienced a post-R3 lineage-specific gene duplication to form paralogous clusters that correlate to the pelagic and benthic character of the eggs. Neo-functionalization allowed one paralogue to be proteolyzed to FAA driving hydration of the maturing oocytes, which pre-adapts them to the marine environment and causes them to float. The timing of these events matches the appearance of the Acanthomorpha in the fossil record. We discuss the significance of these adaptations in relation to ancestral physiological features, and propose that the neo-functionalization of duplicated Vtg genes was a key event in the evolution and success of the teleosts in the oceanic environment
Variant chronic granulomatous disease: modulation of the neutrophil defect by severe infection
Contribution to understanding the evolution of holometaboly: transformation of internal head structures during the metamorphosis in the green lacewing Chrysopa pallens (Neuroptera: Chrysopidae)
Influence of the blood bacterial load on the meningeal inflammatory response in <it>Streptococcus pneumoniae </it>meningitis
<p>Abstract</p> <p>Background</p> <p>Despite bacteraemia is present in the majority of patients with pneumococcal, little is known about the influence of the systemic infection on the meningeal inflammatory response.</p> <p>Methods</p> <p>To explore the role of systemic infection on the meningeal inflammation, experimental meningitis was induced by intracisternal injection of ~1 Ă 10<sup>6 </sup>CFU <it>Streptococcus pneumoniae</it>, type 3, and the 26 rabbits were either provided with ~1 Ă 10<sup>6 </sup>CFU <it>S. pneumoniae </it>intravenously at 0 hour ("bacteraemic" rabbits, n = 9), immunized with paraformaldehyde-killed <it>S. pneumoniae </it>for 5 weeks prior to the experiment ("immunized" rabbits", n = 8), or not treated further ("control" rabbits, n = 9). WBC and bacterial concentrations were determined in CSF and blood every second hour during a 16 hours study period together with CSF IL-8 and protein levels. We also studied CSF and blood WBC levels in 153 pneumococcal meningitis patients with and without presence of bacteraemia.</p> <p>Results</p> <p>As designed, blood bacterial concentrations were significantly different among three experimental groups during the 16 hours study period (Kruskal Wallis test, <it>P </it>< 0.05), whereas no differences in CSF bacterial levels were observed (<it>P </it>> 0.05). Blood WBC decreased in bacteraemic rabbits between ~10â16 hours after the bacterial inoculation in contrast to an increase for both the immunized rabbits and controls (<it>P </it>< 0.05). The CSF pleocytosis was attenuated in bacteraemic rabbits as compared to the two other groups between 12â16 hours from time of infection (<it>P </it>< 0.017), despite accelerated CSF IL-8 levels in bacteraemic rabbits.</p> <p>In patients with pneumococcal meningitis, no significant difference in CSF WBC was observed between patients with or without bacteraemia at admission (n = 103, 1740 cells/ÎźL (123â4032) vs. n = 50, 1961 cells/ÎźL (673â5182), respectively, <it>P </it>= 0.18), but there was a significant correlation between CSF and blood WBC (n = 127, Spearman rho = 0.234, <it>P </it>= 0.008).</p> <p>Conclusion</p> <p>Our results suggest that a decrease in peripheral WBC induced by enhanced bacteraemia in pneumococcal meningitis results in an attenuated CSF pleocytosis.</p
The larval head morphology of Xyela sp (Xyelidae, Hymenoptera) and its phylogenetic implications
Larval head structures of Xyela sp. are described in detail. The characters are compared to conditions found in larvae of other groups of Hymenoptera and Endopterygota. Like other symphytan larvae the immature stages of Xyelidae are mainly characterized by presumably plesiomorphic features of the head. The head sutures are well developed and all parts of the tentorium are present. The labrum is free and a complete set of labral muscles is present. The maxillae are in a retracted position. In contrast to other hymenopteran larvae Xyela possesses a clypeofrontal suture, a comparatively long antenna and three well-developed antennal muscles. Apomorphic features of Xyela are the absence of muscles associated with the salivarium and the complete absence of Musculus craniocardinalis. A clade comprising Orussidae and Apocrita is supported by the unsegmented maxillary and labial palps and the absence of the lacinia. Six potential autapomorphies for the Hymenoptera were revealed: (1) the caudal tentorial apodeme, (2) the bifurcated tendon of Musculus craniomandibularus internus, (3) the lateral lobe of the cardo, (4) the origin of M. tentoriohypopharyngalis from the posterior head capsule, (5) the exceptionally strong prepharyngo-pharyngeal longitudinal muscle and (6) the longitudinal muscle of the silk press. The maxillolabial complex, the vestigial M. craniocardinalis and a distinctly developed labio-hypopharyngeal lobe bearing the opening of the salivary duct are potential synapomorphies of Hymenoptera and Mecopterida. The globular, orthognathous head capsule, the modified compound eyes, the occipital furrow and the X-shaped tentorium are features with unclear polarity shared by Hymenoptera and Mecoptera.R. G. Beutel, L. Krogmann and L. Vilhelmse