Experimental characterisation of fatigue crack growth based on the CTOD measured from crack tip displacement fields using DIC

The current work presents an experimental study on the use of the crack tip opening displacement (CTOD) to evaluate its ability to characterise fatigue crack growth. A methodology is developed to measure and to analyse the CTOD from experimental data. The vertical displacements obtained by implementing digital image correlation (DIC) on growing fatigue cracks are used to measure the CTOD. Two fatigue tests at stress ratios of 0.1 and 0.6 were conducted on compact tension (CT) specimens manufactured from a 1 mm thick sheet of commercially pure titanium. A sensitivity analysis to explore the effect of the position selected behind the crack tip for the CTOD measurement was performed. The analysis of a full loading cycle allowed identifying the elastic and plastic components of the CTOD. The plastic CTOD was found to be directly related to the nonlinear zone (i.e., plastic deformation) generated at the crack tip during fatigue propagation. Moreover, a linear relationship between da/dN and ΔCTODp independent of the stress ratio was found. Results show that the CTOD can be used as a viable alternative to the stress intensity factor range (ΔK) in characterising fatigue crack propagation since the parameter considers the fatigue threshold and crack shielding in an intrinsic way

Effect Of CARICAPRYL-99 Seed Alkaloid Extract On The Serum Levels Of Sex Hormones And Pituitary Gonadotrophins In Male Albino Rats

Summary: Activity of alkaloid extract of caricapryl–99 seeds (Carica papaya Linn seeds) on the serum levels of steroid hormones was studied in adult male albino rats. Three tolerated doses obtained from the graph of percectage toxicity (10, 50 and 150 mg/kg) were separately administered orally, daily for three days to three groups of male rats (n=5) while group four of 5 rats received the vehicle (corn oil) as control. The results showed that 10mg/kg/d caused increase serum levels of FSH and estrogen but decrease in the serum levels of LH and testosterone compared to control; 50mg/kg/d elevated the serum levels of FSH, estrogen but inhibited testosterone; while 150mg/kg/d pretreatments caused a significant decrease (

Experimental evaluation of CTOD in constant amplitude fatigue crack growth from crack tip displacement fields

© 2017, Gruppo Italiano Frattura. All rights reserved. In the current work an experimental study of the crack tip opening displacement (CTOD) is performed to evaluate the ability of this parameter to characterise fatigue crack growth. A methodology is developed to measure and to analyse the CTOD from experimental data. The vertical displacements measured by implementing Digital Image Correlation on growing fatigue cracks are used to measure the CTOD. Fatigue tests at R ratios of 0.1 and 0.6 were conducted on compact-tension specimens manufactured from commercially pure titanium. A sensitivity analysis was performed to explore the effect of the position selected behind the crack tip for the CTOD measurement. The analysis of a full loading cycle allowed identifying the elastic and plastic components of the CTOD. The plastic CTOD was found to be directly related to the plastic deformation at the crack tip. Moreover, a linear relationship between da/dN and the plastic CTOD for both tests was observed. Results show that the CTOD can be used as a viable alternative to ΔK in characterising fatigue crack propagation because the parameter considers fatigue threshold and crack shielding in an intrinsic way. This work is intended to contribute to a better understanding of the different mechanisms driving fatigue crack growth and the address the outstanding controversy associated with plasticity-induced fatigue crack closure

Characterisation of fatigue crack growth using the CJP model of crack tip fields or plastic CTOD

This paper provides an update on the current status of work that has been done to validate the CJP model of crack tip stresses and also summarises some findings from linked work that has used DIC to determine the range of plastic crack tip opening displacement as a correlator of fatigue crack growth rate. The paper considers several ways of calculating an effective range of stress intensity factor that have been proposed in various papers, as the preferred option is not yet fully clear. It further highlights the potential value, in terms of elucidating the mechanisms involved in plasticity-induced crack tip shielding, arising from data obtained from using two different DIC techniques on the same specimens

Evaluation of small-scale yielding boundary using digital image correlation results

In this work, the boundaries of small-scale yielding (SSY) and large-scale yielding (LSY) have been experimentally evaluated from the analysis of crack tip opening displacement (CTOD) measured by digital image correlation (DIC). According to a previous numerical work, the ratio between the elastic CTOD range and the total CTOD range (Δδe/Δδt) defines the boundaries of SSY conditions and LSY. Three materials have been studied, commercially pure titanium and 2024-T3 and 7050-T6 aluminum alloys, tested at different stress ratio values. SSY conditions are shown to dominate when Δδe/Δδt ≥ 79% and ≥78% for titanium and the two aluminum alloys, respectively. In addition, LSY can be established when Δδe/Δδt ≤ 66.3% and ≤67.2% for titanium and for 2024-T3 and 7050-T6 aluminum alloys, respectively. Transition or LSY conditions are more probable in fatigue tests conducted at low R ratio and for crack lengths above 40% with respect to the width of the specimen

Are genetic risk factors for psychosis also associated with dimension-specific psychotic experiences in adolescence?

Psychosis has been hypothesised to be a continuously distributed quantitative phenotype and disorders such as schizophrenia and bipolar disorder represent its extreme manifestations. Evidence suggests that common genetic variants play an important role in liability to both schizophrenia and bipolar disorder. Here we tested the hypothesis that these common variants would also influence psychotic experiences measured dimensionally in adolescents in the general population. Our aim was to test whether schizophrenia and bipolar disorder polygenic risk scores (PRS), as well as specific single nucleotide polymorphisms (SNPs) previously identified as risk variants for schizophrenia, were associated with adolescent dimension-specific psychotic experiences. Self-reported Paranoia, Hallucinations, Cognitive Disorganisation, Grandiosity, Anhedonia, and Parent-rated Negative Symptoms, as measured by the Specific Psychotic Experiences Questionnaire (SPEQ), were assessed in a community sample of 2,152 16-year-olds. Polygenic risk scores were calculated using estimates of the log of odds ratios from the Psychiatric Genomics Consortium GWAS stage-1 mega-analysis of schizophrenia and bipolar disorder. The polygenic risk analyses yielded no significant associations between schizophrenia and bipolar disorder PRS and the SPEQ measures. The analyses on the 28 individual SNPs previously associated with schizophrenia found that two SNPs in TCF4 returned a significant association with the SPEQ Paranoia dimension, rs17512836 (p-value=2.57x10-4) and rs9960767 (p-value=6.23x10-4). Replication in an independent sample of 16-year-olds (N=3,427) assessed using the Psychotic-Like Symptoms Questionnaire (PLIKS-Q), a composite measure of multiple positive psychotic experiences, failed to yield significant results. Future research with PRS derived from larger samples, as well as larger adolescent validation samples, would improve the predictive power to test these hypotheses further. The challenges of relating adult clinical diagnostic constructs such as schizophrenia to adolescent psychotic experiences at a genetic level are discussed

Behaviour and Physiology: The Thermal Strategy of Leatherback Turtles

Background: Adult leatherback turtles (Dermochelys coriacea) exhibit thermal gradients between their bodies and the environment of $8uC in sub-polar waters and #4uC in the tropics. There has been no direct evidence for thermoregulation in leatherbacks although modelling and morphological studies have given an indication of how thermoregulation may be achieved. Methodology/Principal Findings: We show for the first time that leatherbacks are indeed capable of thermoregulation from studies on juvenile leatherbacks of 16 and 37 kg. In cold water (, 25uC), flipper stroke frequency increased, heat loss through the plastron, carapace and flippers was minimized, and a positive thermal gradient of up to 2.3uC was maintained between body and environment. In warm water (25 – 31uC), turtles were inactive and heat loss through their plastron, carapace and flippers increased. The thermal gradient was minimized (0.5uC). Using a scaling model, we estimate that a 300 kg adult leatherback is able to maintain a maximum thermal gradient of 18.2uC in cold sub-polar waters. Conclusions/Significance: In juvenile leatherbacks, heat gain is controlled behaviourally by increasing activity while heat flux is regulated physiologically, presumably by regulation of blood flow distribution. Hence, harnessing physiology and behaviour allows leatherbacks to keep warm while foraging in cold sub-polar waters and to prevent overheating in

Fructose transport-deficient Staphylococcus aureus reveals important role of epithelial glucose transporters in limiting sugar-driven bacterial growth in airway surface liquid.

Hyperglycaemia as a result of diabetes mellitus or acute illness is associated with increased susceptibility to respiratory infection with Staphylococcus aureus. Hyperglycaemia increases the concentration of glucose in airway surface liquid (ASL) and promotes the growth of S. aureus in vitro and in vivo. Whether elevation of other sugars in the blood, such as fructose, also results in increased concentrations in ASL is unknown and whether sugars in ASL are directly utilised by S. aureus for growth has not been investigated. We obtained mutant S. aureus JE2 strains with transposon disrupted sugar transport genes. NE768(fruA) exhibited restricted growth in 10 mM fructose. In H441 airway epithelial-bacterial co-culture, elevation of basolateral sugar concentration (5-20 mM) increased the apical growth of JE2. However, sugar-induced growth of NE768(fruA) was significantly less when basolateral fructose rather than glucose was elevated. This is the first experimental evidence to show that S. aureus directly utilises sugars present in the ASL for growth. Interestingly, JE2 growth was promoted less by glucose than fructose. Net transepithelial flux of D-glucose was lower than D-fructose. However, uptake of D-glucose was higher than D-fructose across both apical and basolateral membranes consistent with the presence of GLUT1/10 in the airway epithelium. Therefore, we propose that the preferential uptake of glucose (compared to fructose) limits its accumulation in ASL. Pre-treatment with metformin increased transepithelial resistance and reduced the sugar-dependent growth of S. aureus. Thus, epithelial paracellular permeability and glucose transport mechanisms are vital to maintain low glucose concentration in ASL and limit bacterial nutrient sources as a defence against infection