Estrutura de tamanho e fertilidade em uma população de Eriocnema fulva Naudin (Melastomataceae) no sudeste do Brasil

Eriocnema fulva Naudin is an endangered perennial herbaceous plant, endemic to Minas Gerais state, Brazil. This study was conducted in the Jambreiro Forest (19° 58' -59'S and 43° 52' -55' W, 800-1100 m altitude). In an attempt to describe the population size structure and its association with individual fertility, fifteen 1 x 1 m contiguous plots were set. We tagged, counted, and measured a total of 260 individuals in 1997, 1998 and 1999. Young individuals with leaf lamina lengths 10 cm in length did not reproduce was 2.69%. The highest survival probability of the large-sized individuals confirmed the strong correlation between size and survival. The data indicated that size is important for the fertility of E. fulva, and it may be one of the relevant aspects to be considered for analyses of survival probability. The intraspecific competition, which was indicated by negative correlation between fruit production per size unit and density, can affect fertility, as larger plants had higher fruit production.Eriocnema fulva é endêmica no estado de Minas Gerais e é ameaçada de extinção. Este estudo foi realizado na Mata do Jambreiro (19° 58' -59' S e 43° 52'-55' W, 800-1100 m de altitude) Com o objetivo de descrever a estrutura de tamanho da população e suas relações com a fertilidade dos indivíduos, foram instaladas 15 parcelas contíguas de 1 x 1m. Marcamos, contamos e medimos um total de 260 indivíduos em 1997, 1998 e 1999. Os indivíduos jovens com o comprimento do limbo 10 cm não se reproduzirem foi de 2,69%. A maior probabilidade de sobrevivência dos indivíduos de maior tamanho confirmou a forte correlação entre tamanho e sobrevivência. Os dados mostraram que o tamanho é importante para a fertilidade de Eriocnema fulva e pode ser relevante para análises de probabilidade de sobrevivência da espécie. A competição intra-específica, indicada pela correlação negativa entre a produção de frutos por unidade de tamanho e a densidade, pode influir na fertilidade, pois plantas maiores apresentaram maior produção de frutos.685693Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Crescimento foliar de Eriocnema fulva Naudin (Melastomataceae) em um fragmento florestal no Brasil sudeste

Eriocnema fulva Naudin is an endangered herbaceous, perennial, iteroparous, evergreen species geographically restricted to southeastern-center Minas Gerais State, SE Brazil. The individuals occur as patches on rocky riverbanks shaded by seasonal semideciduous Atlantic forest; they are fixed by roots and have a pending stem. Aiming to investigate leaf development and its importance for individual survival, fifteen contiguous plots (1 x 1 m) were set down in Jambreiro Forest (19° 58?-59? S and 43° 52?-55? W, 800-1100 m altitude), in the municipality of Nova Lima. A total of 260 individuals with the largest leaf blade length > 1 cm was tagged and measured in 1997, 1998, and 1999. Leaf expansion was recorded each month during 26 months until April 2000. Plant size was measured through leaf blade length, petiole length, stem length, and number of leaves. Significant changes were detected only after two years, thus indicating that plant growth is slow. The proportion of surviving leaves after two years was 60%. Total blade expansion took over 14 months, a slow growth rate when compared to leaves of other tropical forest canopy and understory species. Long leaf lifespans are to be found in plants exhibiting slow growth, and we observed that some leaves lived longer than three years. Petiole growth can help to better position the leaf in the search for light, thus contributing to the growth and survival of the plant. The relationships among size measures were significant, reinforcing the great contribution of leaf size for plant size. The age of the largest individual was estimated as 36 years based on the median annual leaf production rate.Eriocnema fulva é uma espécie herbácea, perene, iteropárica, com distribuição geográfica restrita ao centro-sudeste do Estado de Minas Gerais, Brasil, e ameaçada de extinção. Os indivíduos ocorrem agrupados em paredões rochosos, na beira de riachos sombreados pela Mata Atlântica estacional semidecídua e têm caule que pendem do barranco. Com o objetivo de investigar o desenvolvimento da folha e sua importância para a sobrevivência dos indivíduos, foram instaladas 15 parcelas contíguas de 1 x 1 m na Mata do Jambreiro (19° 58?-59?S, 43° 52?-55? W, 800-1100 m de altitude), município de Nova Lima. Todos os indivíduos cuja maior folha tivesse comprimento do limbo maior ou igual a 1 cm foram marcados, sendo medidos em 1997, 1998 e 1999. O tempo de expansão da folha foi registrado mensalmente durante 26 meses até abril de 2000. Na área amostrada foram marcados 260 indivíduos cujo limbo foliar tinha comprimento > 1cm. O tamanho das plantas, medido através do comprimento do limbo e do pecíolo e do número de folhas, apresentou mudanças significativas somente após dois anos, indicando que o crescimento é lento. A proporção das folhas sobreviventes após dois anos foi de 60%. O tempo necessário para a expansão da folha foi superior a 14 meses e é lento quando comparado com o de outras espécies do dossel ou do sub-bosque da floresta tropical. Maior longevidade foliar tende a ser encontrada em plantas de crescimento lento. Observamos que algumas folhas tiveram maior longevidade que 3 anos. O crescimento do pecíolo pode ajudar no melhor posicionamento da folha na busca de luz no sub-bosque, contribuindo para o crescimento e sobrevivência da planta. As relações entre a folha e demais medidas de tamanho foram significativas, indicando sua grande importância para o tamanho de cada planta. A idade do indivíduo de maior tamanho foi estimada em 36 anos através da taxa mediana de produção anual de folhas.110Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Contribution of microscopy for understanding the mechanism of action against trypanosomatids

Transmission electron microscopy (TEM) has proved to be a useful tool to study the ultrastructural alterations and the target organelles of new antitrypanosomatid drugs. Thus, it has been observed that sesquiterpene lactones induce diverse ultrastructural alterations in both T. cruzi and Leishmania spp., such as cytoplasmic vacuolization, appearance of multilamellar structures, condensation of nuclear DNA, and, in some cases, an important accumulation of lipid vacuoles. This accumulation could be related to apoptotic events. Some of the sesquiterpene lactones (e.g., psilostachyin) have also been demonstrated to cause an intense mitochondrial swelling accompanied by a visible kinetoplast deformation as well as the appearance of multivesicular bodies. This mitochondrial swelling could be related to the generation of oxidative stress and associated to alterations in the ergosterol metabolism. The appearance of multilamellar structures and multiple kinetoplasts and flagella induced by the sesquiterpene lactone psilostachyin C indicates that this compound would act at the parasite cell cycle level, in an intermediate stage between kinetoplast segregation and nuclear division. In turn, the diterpene lactone icetexane has proved to induce the external membrane budding on T. cruzi together with an apparent disorganization of the pericellar cytoskeleton. Thus, ultrastructural TEM studies allow elucidating the possible mechanisms and the subsequent identification of molecular targets for the action of natural compounds on trypanosomatids.Fil: Lozano, Esteban Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Spina Zapata, Renata María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Barrera, Patricia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Tonn, Carlos Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentin

Literature-based discovery of diabetes- and ROS-related targets

Abstract Background Reactive oxygen species (ROS) are known mediators of cellular damage in multiple diseases including diabetic complications. Despite its importance, no comprehensive database is currently available for the genes associated with ROS. Methods We present ROS- and diabetes-related targets (genes/proteins) collected from the biomedical literature through a text mining technology. A web-based literature mining tool, SciMiner, was applied to 1,154 biomedical papers indexed with diabetes and ROS by PubMed to identify relevant targets. Over-represented targets in the ROS-diabetes literature were obtained through comparisons against randomly selected literature. The expression levels of nine genes, selected from the top ranked ROS-diabetes set, were measured in the dorsal root ganglia (DRG) of diabetic and non-diabetic DBA/2J mice in order to evaluate the biological relevance of literature-derived targets in the pathogenesis of diabetic neuropathy. Results SciMiner identified 1,026 ROS- and diabetes-related targets from the 1,154 biomedical papers (http://jdrf.neurology.med.umich.edu/ROSDiabetes/). Fifty-three targets were significantly over-represented in the ROS-diabetes literature compared to randomly selected literature. These over-represented targets included well-known members of the oxidative stress response including catalase, the NADPH oxidase family, and the superoxide dismutase family of proteins. Eight of the nine selected genes exhibited significant differential expression between diabetic and non-diabetic mice. For six genes, the direction of expression change in diabetes paralleled enhanced oxidative stress in the DRG. Conclusions Literature mining compiled ROS-diabetes related targets from the biomedical literature and led us to evaluate the biological relevance of selected targets in the pathogenesis of diabetic neuropathy.http://deepblue.lib.umich.edu/bitstream/2027.42/78315/1/1755-8794-3-49.xmlhttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/2/1755-8794-3-49-S7.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/3/1755-8794-3-49-S10.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/4/1755-8794-3-49-S8.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/5/1755-8794-3-49-S3.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/6/1755-8794-3-49-S1.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/7/1755-8794-3-49-S4.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/8/1755-8794-3-49-S2.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/9/1755-8794-3-49-S12.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/10/1755-8794-3-49-S11.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/11/1755-8794-3-49-S9.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/12/1755-8794-3-49-S5.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/13/1755-8794-3-49-S6.XLShttp://deepblue.lib.umich.edu/bitstream/2027.42/78315/14/1755-8794-3-49.pdfPeer Reviewe

The MHC Gene Region of Murine Hosts Influences the Differential Tissue Tropism of Infecting Trypanosoma cruzi Strains

We have previously demonstrated that both parasite genetic variability and host genetic background were important in determining the differential tissue distribution of the Col1.7G2 and JG T. cruzi monoclonal strains after artificial infections in mice. We observed that the JG strain was most prevalent in hearts of mouse lineages with the MHC haplotype H-2d (BALB/c and DBA2), while Col1.7G2 was predominant in hearts from C57BL/6 mice, which have the H-2b haplotype. To assess whether the MHC gene region indeed influenced tissue tropism of T. cruzi, we used the same two parasite strains to infect C57BL/6 (H-2b) and C57BLKS/J (H-2d) mice; the latter strain results from the introgression of DBA2 MHC region into the C57BL/6 background. We also performed ex vivo infections of cardiac explants from four congenic mice lineages with the H-2b and H-2d haplotypes arranged in two different genetic backgrounds: C57BLKS/J (H-2d) versus C57BL/6 (H-2b) and BALB/c (H-2d) versus BALB/B10-H2b (H-2b). In agreement with our former observations, Col1.7G2 was predominant in hearts from C57BL/6 mice (H-2b), but we observed a clear predominance of the JG strain in hearts from C57BLKS/J animals (H-2d). In the ex vivo experiments Col1.7G2 also prevailed in explants from H-2b animals while no predominance of any of the strains was observed in H-2d mice explants, regardless of the genetic background. These observations clearly demonstrate that the MHC region influences the differential tissue distribution pattern of infecting T. cruzi strains, which by its turn may be in a human infection the determinant for the clinical forms of the Chagas disease

Insights into the Function of the CRM1 Cofactor RanBP3 from the Structure of Its Ran-Binding Domain

Proteins bearing a leucine-rich nuclear export signal (NES) are exported from the nucleus by the transport factor CRM1, which forms a cooperative ternary complex with the NES-bearing cargo and with the small GTPase Ran. CRM1-mediated export is regulated by RanBP3, a Ran-interacting nuclear protein. Unlike the related proteins RanBP1 and RanBP2, which promote disassembly of the export complex in the cytosol, RanBP3 acts as a CRM1 cofactor, enhancing NES export by stabilizing the export complex in the nucleus. RanBP3 also alters the cargo selectivity of CRM1, promoting recognition of the NES of HIV-1 Rev and of other cargos while deterring recognition of the import adaptor protein Snurportin1. Here we report the crystal structure of the Ran-binding domain (RBD) from RanBP3 and compare it to RBD structures from RanBP1 and RanBP2 in complex with Ran and CRM1. Differences among these structures suggest why RanBP3 binds Ran with unusually low affinity, how RanBP3 modulates the cargo selectivity of CRM1, and why RanBP3 promotes assembly rather than disassembly of the export complex. The comparison of RBD structures thus provides an insight into the functional diversity of Ran-binding proteins