35 research outputs found
In vitro synergistic cytoreductive effects of zoledronic acid and radiation on breast cancer cells
INTRODUCTION: Bisphosphonates are mostly used in the treatment of bone metastases. They have been shown to act synergistically with other chemotherapeutic agents. It is not known, however, whether similar synergistic effects exist with radiation on breast cancer cells. METHODS: Human MCF-7 breast cancer cells were treated with up to 100 ÎŒM zoledronic acid, were irradiated with up to 800 cGy or were exposed to combinations of both treatments to determine the antiproliferative effects of zoledronic acid and radiation. RESULTS: Zoledronic acid and radiation caused a dose-dependent and time-dependent decrease in cell viability (approximate 50% growth inhibition values were 48 ÎŒM and 20 ÎŒM for 24 hours and 72 hours, respectively, for zoledronic acid and 500 cGy for radiation). A synergistic cytotoxic effect of the combination of zoledronic acid and radiation was confirmed by isobologram analysis. CONCLUSION: These data constitute the first in vitro evidence for synergistic effects between zoledronic acid and radiation. This combination therapy might thus be expected to be more effective than either treatment alone in patients with metastatic breast carcinoma
Long-Term Climate Forcing in Loggerhead Sea Turtle Nesting
The long-term variability of marine turtle populations remains poorly understood,
limiting science and management. Here we use basin-scale climate indices and
regional surface temperatures to estimate loggerhead sea turtle (Caretta
caretta) nesting at a variety of spatial and temporal scales.
Borrowing from fisheries research, our models investigate how oceanographic
processes influence juvenile recruitment and regulate population dynamics. This
novel approach finds local populations in the North Pacific and Northwest
Atlantic are regionally synchronized and strongly correlated to ocean
conditionsâsuch that climate models alone explain up to 88% of the
observed changes over the past several decades. In addition to its performance,
climate-based modeling also provides mechanistic forecasts of historical and
future population changes. Hindcasts in both regions indicate climatic
conditions may have been a factor in recent declines, but future forecasts are
mixed. Available climatic data suggests the Pacific population will be
significantly reduced by 2040, but indicates the Atlantic population may
increase substantially. These results do not exonerate anthropogenic impacts,
but highlight the significance of bottom-up oceanographic processes to marine
organisms. Future studies should consider environmental baselines in assessments
of marine turtle population variability and persistence
Complementary intestinal mucosa and microbiota responses to caloric restriction
The intestine is key for nutrient absorption and for interactions between the microbiota and its host. Therefore, the intestinal response to caloric restriction (CR) is thought to be more complex than that of any other organ. Submitting mice to 25% CR during 14 days induced a polarization of duodenum mucosa cell gene expression characterised by upregulation, and downregulation of the metabolic and immune/inflammatory pathways, respectively. The HNF, PPAR, STAT, and IRF families of transcription factors, particularly the Pparα and Isgf3 genes, were identified as potentially critical players in these processes. The impact of CR on metabolic genes in intestinal mucosa was mimicked by inhibition of the mTOR pathway. Furthermore, multiple duodenum and faecal metabolites were altered in CR mice. These changes were dependent on microbiota and their magnitude corresponded to microbial density. Further experiments using mice with depleted gut bacteria and CR-specific microbiota transfer showed that the gene expression polarization observed in the mucosa of CR mice is independent of the microbiota and its metabolites. The holistic interdisciplinary approach that we applied allowed us to characterize various regulatory aspects of the host and microbiota response to CR
Integrating genetics and epigenetics in breast cancer: biological insights, experimental, computational methods and therapeutic potential
Morphological and biochemical observations on hepatic glycogen metabolism in mice on a controlled feeding schedule
Asma induzida pelo exercĂcio: aspectos atuais e recomendaçÔes Asma inducido por el ejercicio: aspectos actuales y recomendaciones Exercise-induced asthma: current aspects and recommendations
OBJETIVO: Descrever os mecanismos da asma induzida pelo exercĂcio (AIE), bem como os efeitos de diferentes tipos de treinamento fĂsico na função pulmonar e nas capacidades aerĂłbia e anaerĂłbia. Destaca-se a importĂąncia de um diagnĂłstico correto mediante o teste de exercĂcio e, no manejo, o uso de drogas beta-adrenĂ©rgicas e anticolinĂ©rgicas. FONTE DOS DADOS: Os artigos foram criteriosamente escolhidos utilizando as bases de dados PubMed e Scielo pelo ano de publicação e dando preferĂȘncia a ensaios clĂnicos randomizados, com critĂ©rios de seleção da amostra bem definidos. SĂNTESE DOS DADOS: Os mecanismos para explicar a AIE permanecem sem conclusĂŁo, mas parece haver uma interação fisiolĂłgica entre as hipĂłteses aqui apresentadas. O uso de medicamentos e as freqĂŒentes crises durante o exercĂcio aparecem como fatores limitantes para a prĂĄtica de exercĂcios fĂsicos, conduzindo para um estilo de vida sedentĂĄrio. CONCLUSĂO: Deve-se incentivar a prĂĄtica de exercĂcios devidamente prescritos e minimizar as restriçÔes aos sujeitos com AIE.<br>OBJETIVO: Describir los mecanismos del asma inducido por el ejercicio (AIE), asĂ como los efectos de diferentes tipos de entrenamiento fĂsico sobre la funciĂłn pulmonar y las capacidades aerĂłbica y anaerĂłbica. Se destaca la importancia de un diagnĂłstico correcto mediante el test de ejercicio y el manejo o uso de drogas beta-adrenĂ©rgicas y anticolinĂ©rgicas. FUENTES: Los artĂculos fueron cuidadosamente escogidos utilizando las bases de dados de PubMed y Scielo por el año de publicaciĂłn, dando preferencia a ensayos clĂnicos randomizados, con criterios de selecciĂłn de muestra bien definidos. SĂNTESES DE DATOS: Los mecanismos para explicar la AIE permanecen sin conclusiĂłn, pero parece haber una interacciĂłn fisiolĂłgica entre las hipĂłtesis aquĂ presentadas. El uso de medicamentos y las frecuentes crisis durante el ejercicio aparecen como factores limitantes para la prĂĄctica de ejercicios fĂsicos, conduciendo esto a un estilo de vida sedentario. CONCLUSIĂN: Se debe incentivar la prĂĄctica de ejercicios debidamente prescritos y minimizar las restricciones a los individuos con AIE.<br>OBJECTIVE: To describe the mechanisms of Exercise-Induced Asthma (EIA) as well as the effect of different kinds of physical training on pulmonary function, anaerobic fitness, and aerobic fitness. We highlighted the importance of a correct diagnostic through exercise testing and, concerning treatment, the utilization of drugs such as beta-adrenergics and anticholinergics. DATA SOURCE: The articles were chosen using the PubMed and Scielo databases, considering the year of publication and giving preference to clinical randomized trials with well-defined inclusion criteria. SUMMARY OF THE FINDINGS: The medication used and the frequent symptoms during and after exercise appear as a limiting factor to the practice of exercises among subjects with EIA. This may result in a sedentary lifestyle. CONCLUSION: Subjects with EIA should be allowed to do exercise if well prescribed
A systematic review of faculty development activities in family medicine
Background: Faculty development (FD) has been defined as a planned programme to prepare institutions and faculty members for their roles in the areas of teaching, research, administration and career management. However, there are few generalisable evaluations of FD activities available to help family medicine FD planners to choose the most effective training strategies. Aim: To assess the evidence for the effectiveness of family medicine FD activities. Method: Six electronic databases were searched from 1980 to 2010 and included all articles on FD interventions in family medicine. Hand searching was also undertaken. Results: A total of 4520 articles were identified, 46 fulfilled the search criteria and were reviewed across three domains: (a) Context, i.e. setting, participation and funding. (b)Content/Process, i.e. theoretical framework, focus of intervention/learning outcomes, types of FD intervention and instructional methods. (c) Evaluation using Freeth et al's adaptation of Kirkpatrick's outcome levels. Conclusion: FD activities appear highly valued by the participants, leading to changes in learning and behaviour. Changes in organisational practice and student learning were not frequently reported. The continued success of family medicine FD will depend on the contextual approach/collegial support, adaptability of the programmes, robust evaluation and adequate funding in terms of resources and time