Precision teaching and fluency: the effects of charting and goal-setting on skaters’ performance

This research involved three successive studies where precision teaching methods were used to increase roller skaters performance rates of basic skating skills. The first study compared precision teaching methods both with and without the use of charting in a group design, and compared both within-subject and betweensubject differences for 12 skaters. Results showed that charting did not increase performance rates over that seen when charting was not used. The second study was a group design which compared two different types of goals, or performance aims, on skaters‟ performance. No difference was found between the two groups of 5 skaters when one group used a fixed, difficult goal and a second used a flexible, easier goal. In the final study, a single-subject design was used and 8 skaters completed a control condition where no goals were set before a goal was introduced for 4 skaters. It was found that an immediate increase in performance rates occurred following the introduction of the goal. Overall these three studies showed that skaters improved their performance rates over sessions, even in the absence of charting and/or goals, demonstrating that precision teaching can be applied to the sport of roller skating

Extinction-induced variability in human behavior

Participants earned points by pressing a computer space bar (Experiment 1) or forming rectangles on the screen with the mouse (Experiment 2) under differential-reinforcement-of-low-rate schedules, followed by extinction. Variability in interresponse time (the contingent dimension) increased during extinction, as for Morgan and Lee (1996); variability in diagonal length (the noncontingent dimension, Experiment 2) did not. In Experiment 3, points were contingent on rectangle size. Rectangle size and interresponse-time (the noncontingent dimension) variability increased in extinction. There was greater variability in the contingent dimension during extinction for participants with the more varied history of reinforcement in Experiment 2 but not in Experiment 3. Overall, variability in the contingent dimension increased in extinction, but the degree of increase was affected by reinforcement history

Simulation of multi-deck medium temperature display cabinets with the integration of CFD and cooling coil models

This is the post-print version of the final paper published in Applied Energy. The published article is available from the link below. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. Copyright @ 2010 Elsevier B.V.In this paper, the model for the multi-deck medium temperature display cabinets is developed with the integration of CFD and cooling coil sub-models. The distributed method is used to develop the cooling coil model with the airside inputs from the outputs of the CFD model. Inversely, the airside outputs from the cooling coil model are used to update the boundary conditions of the CFD model. To validate this cabinet model, a multi-deck medium temperature display cabinet refrigerated with a secondary refrigerant cooling coil was selected as a prototype and mounted in an air conditioned chamber. Extensive tests were conducted at constant space air temperature and varied relative humilities. The cabinet model has been validated by comparing with the test results for the parameters of air at different locations of the flow path, and temperatures of refrigerant and food product, etc. The validated model is therefore used to explore and analyse the cabinet performance and control strategies at various operating and design conditions.DEFR

Evaluation of silicon MOSFETs and GaN HEMTs in soft‐switched and hard‐switched DC‐DC boost converters for domestic PV applications

Hard‐switched high‐gain DC‐DC converters such as the boost converter play an important role in renewable energy systems. Research to increase their efficiency is important and can be achieved using soft‐switching techniques; however, that approach requires an auxiliary circuit. The auxiliary circuit decreases power density and reliability while increasing the cost. Moreover, soft‐switching topologies usually cannot improve the efficiency for all power and voltage ranges. Wide bandgap (WBG) devices, such as gallium nitride (GaN), result in lower switching losses than silicon (Si), can be used while retaining the simple structure of a hard‐switched topology. However, the high cost of these devices is problematic for their frequently cost‐sensitive applications. To quantify the cost and efficiency, this study compares soft‐switching techniques and WBG‐based switches in DC‐DC boost converters for a photovoltaic (PV) energy application. The performance of four prototypes including the soft‐switched and hard‐switched DC‐DC converters with both state‐of‐the‐art Si and GaN switches are evaluated in terms of cost, power density, efficiency, and reliability using theoretical analysis, simulation and experimental results. It is shown that the GaN‐based hard‐switched converter provides higher efficiency and power density; it is more expensive than its Si‐based counterpart, yet is cheaper than soft‐switched converters

Analysis, design and modelling of two fully- integrated transformers with segmental magnetic shunt for LLC resonant converters

To achieve a precise, high leakage inductance for an integrated magnetic transformer, a magnetic shunt (based on low- permeability materials) is usually added to the planar transformer. However, high-performance low-permeability power materials are not readily available in the market. Therefore, two new topologies for shunt-inserted planar transformer are proposed in this paper. In the proposed topologies, the magnetic shunts are based on high-permeability materials like ferrite, which is widely available, and use multiple small gaps to approximate a low-permeability material as an alternative to a low-permeability magnetic shunt. The analysis, design and modelling of the proposed planar transformers are presented in detail. It is shown that the magnetizing inductance can be controlled by vertical air gaps and the leakage inductance value can be controlled by the thickness of the shunt. Hence, the desirable leakage inductance and magnetizing inductance values for the integrated transformer can be obtained for use in LLC resonant converters. The theoretical analyses are verified by finite element analysis (FEA) and the AC resistance for the proposed topologies is discussed

Inability to sustain intraphagolysosomal killing of Staphylococcus aureus predisposes to bacterial persistence in macrophages

Macrophages are critical effectors of the early innate response to bacteria in tissues. Phagocytosis and killing of bacteria are interrelated functions essential for bacterial clearance but the rate-limiting step when macrophages are challenged with large numbers of the major medical pathogen Staphylococcus aureus is unknown. We show that macrophages have a finite capacity for intracellular killing and fail to match sustained phagocytosis with sustained microbial killing when exposed to large inocula of S. aureus (Newman, SH1000 and USA300 strains). S. aureus ingestion by macrophages is associated with a rapid decline in bacterial viability immediately after phagocytosis. However, not all bacteria are killed in the phagolysosome, and we demonstrate reduced acidification of the phagolysosome, associated with failure of phagolysosomal maturation and reduced activation of cathepsin D. This results in accumulation of viable intracellular bacteria in macrophages. We show macrophages fail to engage apoptosis-associated bacterial killing. Ultittop mately macrophages with viable bacteria undergo cell lysis, and viable bacteria are released and can be internalized by other macrophages. We show that cycles of lysis and reuptake maintain a pool of viable intracellular bacteria over time when killing is overwhelmed and demonstrate intracellular persistence in alveolar macrophages in the lungs in a murine model

Bacterial Cell Enlargement Requires Control of Cell Wall Stiffness Mediated by Peptidoglycan Hydrolases.

Most bacterial cells are enclosed in a single macromolecule of the cell wall polymer, peptidoglycan, which is required for shape determination and maintenance of viability, while peptidoglycan biosynthesis is an important antibiotic target. It is hypothesized that cellular enlargement requires regional expansion of the cell wall through coordinated insertion and hydrolysis of peptidoglycan. Here, a group of (apparent glucosaminidase) peptidoglycan hydrolases are identified that are together required for cell enlargement and correct cellular morphology of Staphylococcus aureus, demonstrating the overall importance of this enzyme activity. These are Atl, SagA, ScaH, and SagB. The major advance here is the explanation of the observed morphological defects in terms of the mechanical and biochemical properties of peptidoglycan. It was shown that cells lacking groups of these hydrolases have increased surface stiffness and, in the absence of SagB, substantially increased glycan chain length. This indicates that, beyond their established roles (for example in cell separation), some hydrolases enable cellular enlargement by making peptidoglycan easier to stretch, providing the first direct evidence demonstrating that cellular enlargement occurs via modulation of the mechanical properties of peptidoglycan. IMPORTANCE: Understanding bacterial growth and division is a fundamental problem, and knowledge in this area underlies the treatment of many infectious diseases. Almost all bacteria are surrounded by a macromolecule of peptidoglycan that encloses the cell and maintains shape, and bacterial cells must increase the size of this molecule in order to enlarge themselves. This requires not only the insertion of new peptidoglycan monomers, a process targeted by antibiotics, including penicillin, but also breakage of existing bonds, a potentially hazardous activity for the cell. Using Staphylococcus aureus, we have identified a set of enzymes that are critical for cellular enlargement. We show that these enzymes are required for normal growth and define the mechanism through which cellular enlargement is accomplished, i.e., by breaking bonds in the peptidoglycan, which reduces the stiffness of the cell wall, enabling it to stretch and expand, a process that is likely to be fundamental to many bacteria

Single‐active switch high‐voltage gain DC–DC converter using a non‐coupled inductor

A single‐active switch high‐voltage gain non‐coupled inductor DC–DC converter is presented. The introduced converter achieves high step‐up gain without using any coupled inductors or transformers, provides high efficiency, and has a simple control system. The converter also achieves low voltage stress on the switch and diodes without clamping circuits, reducing cost, conduction losses, and complexity. The input current of the introduced converter is continuous with low ripple, and is therefore suitable for renewable energy applications in which the fast dynamic response of the converter is necessary. The principle of operation and design considerations of the introduced converter are investigated. A 200 W prototype circuit with 40 kHz switching frequency, 40 V input voltage, and 250 V output voltage is implemented. The prototype operates at 93.2% efficiency, with voltage and current error of less than 4% compared to theoretical values

The role of macrophages in Staphylococcus aureus infection

Staphylococcus aureus is a member of the human commensal microflora that exists, apparently benignly, at multiple sites on the host. However, as an opportunist pathogen it can also cause a range of serious diseases. This requires an ability to circumvent the innate immune system to establish an infection. Professional phagocytes, primarily macrophages and neutrophils, are key innate immune cells which interact with S. aureus, acting as gatekeepers to contain and resolve infection. Recent studies have highlighted the important roles of macrophages during S. aureus infections, using a wide array of killing mechanisms. In defense, S. aureus has evolved multiple strategies to survive within, manipulate and escape from macrophages, allowing them to not only subvert but also exploit this key element of our immune system. Macrophage-S. aureus interactions are multifaceted and have direct roles in infection outcome. In depth understanding of these host-pathogen interactions may be useful for future therapeutic developments. This review examines macrophage interactions with S. aureus throughout all stages of infection, with special emphasis on mechanisms that determine infection outcome

Accelerated low-intensity rTMS does not rescue anxiety behaviour or abnormal connectivity in young adult rats following chronic restraint stress

Currently approved repetitive transcranial magnetic stimulation (rTMS) protocols for the treatment of major depressive disorder (MDD) involve once-daily (weekday) stimulation sessions, with 10 Hz or intermittent theta burst stimulation (iTBS) frequencies, over 4–6 weeks. Recently, accelerated treatment protocols (multiple daily stimulation sessions for 1–2 weeks) have been increasingly studied to optimize rTMS treatments. Accelerated protocols might confer unique advantages for adolescents and young adults but there are many knowledge gaps related to dosing in this age group. Off-label, clinical practice frequently outpaces solid evidence as rigorous clinical trials require substantial time and resources. Murine models present an opportunity for high throughput dose finding studies to focus subsequent clinical trials in humans. This project investigated the brain and behavioural effects of an accelerated low-intensity rTMS (LI-rTMS) protocol in a young adult rodent model of chronic restraint stress (CRS). Depression and anxiety-related behaviours were induced in young adult male Sprague Dawley rats using the CRS model, followed by the 3-times-daily delivery of 10 Hz LI-rTMS, for two weeks. Behaviour was assessed using the Elevated Plus Maze and Forced Swim Test, and functional, chemical, and structural brain changes measured using magnetic resonance imaging techniques. CRS induced an agitated depression-like phenotype but therapeutic effects from the accelerated protocol were not detected. Our findings suggest that the age of rodents may impact response to CRS and LI-rTMS. Future studies should also examine higher intensities of rTMS and accelerated theta burst protocols