TURKIYE KLINIKLERI TIP BILIMLERI DERGISI

Objective: Leukemias and non-Hodgkin lymphomas commonly involve the spleen or originate primarily in the spleen and then spread to other sites. Material and Methods: In this retrospective study, we examined the histopathological and immunohistochemical characteristics of 32 cases of primary or secondary splenic B-cell lymphoma and leukemia, in which the diagnosis was established according to the World Health Organization (WHO) classification. The immunohistochemical panel included ALK-1, BCL-2, BCL-6, CD3, CD5, CD10, CD20, CD21, CD23, CD30, CD43, cyclin D1, Ki-67, and TRAP. Results: There was no other nodal or extranodal disease involvement in the majority of patients diagnosed with lymphoma at the time of presentation, while cases of leukemia had undergone splenectomy for palliative purposes. The diagnoses were as follows: 11 cases of hairy cell leukemia (HCL, 34.4%), 8 cases of splenic marginal zone lymphoma (SMZL, 25%), 8 cases of diffuse large B-cell lymphoma (DLBCL, 25%) including 1 T-cell-rich B-cell lymphoma (TCRBCL), 4 cases of mantle cell lymphoma (MCL, 12.5%), and 1 prolymphocytic leukemia (PLL, 3.1%). Conclusion: Overall assessment of spleen, liver, bone marrow, and lymph node examinations and a detailed correlation of the histopathological and immunohistochemical features with the clinical findings are very helpful and usually lead to the final diagnosis in most cases of primary or secondary splenic B-cell lymphoma and leukemia

of Medicine Freshers

Objective: Smoking is the leading preventable cause of death in the world. The prevalence of smoking among medical students in Turkey ranged from 11.8% to 44.2%. The aim of this study was to determine the factors associated with smoking among Adnan Menderes University Faculty of Medicine freshers.Materials and Methods: This study was a cross sectional study. A 22-item questionnaire was used to collect data. Descriptive data are shown in percent. Median, minimum and maximum values are given. Chi-square test was used in the analytical assessment. Type 1 error level is taken as alpha= 0.05.Results: The number of students participating in the study was 188. 55.9% of the students were female. The median age of the students was 19 years (17-22). 24.8% of students (n= 46) reported cigarette use at some points in their lives. 10.8% of students were regular smokers, 7.6% reported smoking occasionally, and 6.4% stated that they had quit smoking. 18.7% of 16 subjects who reported interest in quitting were not aware of the cessation methods. 78.3% of students reported not being affected by the images and slogans on cigarette packages. 46.% of the students stated that being in a circle of smoking friends increased cigarette consumption. Nonetheless, the circle of friends was the most important group with 75% of among whom supporting to quit smoking.Conclusion: Currently, one out of every five medical students is a smoker. This rate is expected to be higher in upper-class students. Efforts to prevent smoking and promote and support smoking among medical students are of great importance. Further studies including regular and occasional smokers and those who consider quitting are needed

Aquaporin-3-mediated hydrogen peroxide transport is required for NF-κB signalling in keratinocytes and development of psoriasis

Aquaporin 3 (AQP3), a water/glycerol channel protein, has been found to transport hydrogen peroxide (H2O2). Here, we show that H2O2, imported via AQP3, is involved in nuclear factor-κB (NF-κB) signalling in keratinocytes and in the pathogenesis of psoriasis. IL-23-mediated induction of psoriasis is reduced in AQP3 knockout mice (AQP3(-/-)), and is accompanied by impaired NF-κB activation and intracellular H2O2 accumulation. In primary keratinocyte cultures, cellular import of H2O2 produced by membrane NADPH oxidase 2 (Nox2) in response to TNF-α is facilitated by AQP3 and required for NF-κB activation by regulation of protein phosphatase 2A. As AQP3 associates with Nox2, we propose that this interplay constitutes H2O2-mediated signalling in response to TNF-α stimulation. Collectively, these data indicate that AQP3-facilitated H2O2 transport is required for NF-κB activation in keratinocytes in the development of psoriasis