Evolución de las infecciones de transmisión sexual en la ciudad de Valencia. Periodo 2004-2009

Las infecciones de transmisión sexual (ITS) han aumentado en los últimos años en toda la Unión Europea. La puesta en marcha de sistemas de notificación y registro de las enfermedades de declaración obligatoria (AVE) y sistemas de notificación automatizada de resultados de microbiología(RedMiVa) han permitido una mejora de la exhaustividad en la detección de casos y por tanto una mejor identificación y prevención de la transmisión de estas enfermedades.Un diseño clásico de vigilancia de salud pública para los registros de Sífilis del periodo 2005-2009 y de Infección gonocócica del periodo 2004-2009, nos permite analizar las características de los sujetos, su evolución temporal y hábitos sexuales en la ciudad de Valencia. Durante el periodo fueron notificados 321 casos de Sífilis y 596 de Infección Gonocócica. La evolución temporal de la incidencia pasó de 2,13 (2005) a 15,60 (2009) para la Sífilis; y de 2,13 (2004) a 26,36 (2009) para la Infección gonocócica. La introducción de la notificación microbiológica incrementó la detección de nuevos casos de ITS.La desproporción por género muestra una evolución creciente de la Razón de masculinidad. La Sífilis presenta cifras superiores entre los inmigrantes tanto en varones como en mujeres, mientras que para la Infección Gonocócica destaca una incidencia superior en mujeres autóctonas que entre las inmigrantes. El factor sexual de riesgomás frecuente para ambas enfermedades fue la existencia de parejas múltiples. El 42,68% de los pacientes con Sífilis y el 36,91% de los pacientes con Infección Gonocócica indicaron esta conducta.La tendencia creciente observada, con independencia de lo anterior, es concordante con informes de similar origen en nuestro país. No obstante, nuestras cifras de Incidencia Anual son sustancialmente mayores que las registradas en el conjunto de la nación.Esta evolución sugiere la necesidad de incrementar las  estrategias activas de prevención y uso de preservativos. De igual modo la detección temprana y el tratamiento correcto de las ITS requieren, en nuestra opinión, unamayor difusión de la información epidemiológica sobre ITS a los profesionales clínicos

Bioelectrical understanding and engineering of cell biology

The last five decades of molecular and systems biology research have provided unprecedented insights into the molecular and genetic basis of many cellular processes. Despite these insights, however, it is arguable that there is still only limited predictive understanding of cell behaviours. In particular, the basis of heterogeneity in single-cell behaviour and the initiation of many different metabolic, transcriptional or mechanical responses to environmental stimuli remain largely unexplained. To go beyond the status quo, the understanding of cell behaviours emerging from molecular genetics must be complemented with physical and physiological ones, focusing on the intracellular and extracellular conditions within and around cells. Here, we argue that such a combination of genetics, physics and physiology can be grounded on a bioelectrical conceptualization of cells. We motivate the reasoning behind such a proposal and describe examples where a bioelectrical view has been shown to, or can, provide predictive biological understanding. In addition, we discuss how this view opens up novel ways to control cell behaviours by electrical and electrochemical means, setting the stage for the emergence of bioelectrical engineering

ALTAS HOSPITALARIAS EN POBLACIÓN INMIGRANTE EN 2006-2010 VS. 2001-2002 EN LA CIUDAD DE VALENCIA

A lo largo de la década, la perspectiva social de la inmigración cobra fuerza frente a la pérdida de interés en el estudio de enfermedades infecto-contagiosas que en un principio suscitaron la investigación sobre posibles enfermedades re-emergentes, presumiblemente importadas y “peligrosas” para la salud pública, aspecto que se ha convertido en anecdótico. Nuestro objetivo ha sido caracterizar la demanda hospitalaria y su variación respecto del inicio de la década. Se desarrolló un estudio observacional transversal para el periodo 01/01/06 al 31/12/10, al objeto de cuantificar las causas de hospitalización y los diagnósticos al alta de la población inmigrante atendida en los hospitales públicos de la ciudad de Valencia. Se presentan las frecuencias comparadas con el año 2003: edad, su media y mediana; género y su proporción; proporción de altas hospitalarias por regiones geográficas y proporción de los diez primeros diagnósticos de alta agrupados por categorías diagnósticas. Se recuperaron 22.874 diagnósticos al alta que Correspondieron al marco geográfico y censal de la ciudad de Valencia. La proporción de mujeres se ha incrementado en 4 puntos porcentuales respecto de 2002. Los inmigrantes de origen centro y sudamericano predominan tanto al inicio de la década, 58,66%, como en 2010, 67,09%. La Categoría “Embarazo y Parto” corresponde en ambos estudios a la causa con mayor proporción de altas (37,71% en 2002 vs. 43,95% en 2010). Los Tumores que pasan del séptimo lugar en 2002 (3,83%) al quinto en 2010 (5.30%). Las enfermedades infecciosas suponían un 4,38% al principio de la década y representan en la actualidad un 3,22%, en ambos casos el sexto lugar. La TBC constituye la enfermedad infecciosa más prevalente, con un 31,58% de altas infecciosas, frente a un 18,2% en 2002. La conclusión principal, relacionada con nuestro objetivo, es que la población es ahora todavía más joven que la nacional, por lo que se espera que esta característica dé lugar a unas menores necesidades de atención sanitaria. Coincidimos de nuevo con múltiples autores que inmigración no es sinónimo de problemas sanitarios, sino de retos médicos y sociales

A Highly Sensitive Assay for Monitoring the Secretory Pathway and ER Stress

Background: The secretory pathway is a critical index of the capacity of cells to incorporate proteins into cellular membranes and secrete proteins into the extracellular space. Importantly it is disrupted in response to stress to the endoplasmic reticulum that can be induced by a variety of factors, including expression of mutant proteins and physiologic stress. Activation of the ER stress response is critical in the etiology of a number of diseases, such as diabetes and neurodegeneration, as well as cancer. We have developed a highly sensitive assay to monitor processing of proteins through the secretory pathway and endoplasmic reticulum (ER) stress in real-time based on the naturally secreted Gaussia luciferase (Gluc). Methodology/Principle Findings: An expression cassette for Gluc was delivered to cells, and its secretion was monitored by measuring luciferase activity in the conditioned medium. Gluc secretion was decreased down to 90% when these cells were treated with drugs that interfere with the secretory pathway at different steps. Fusing Gluc to a fluorescent protein allowed quantitation and visualization of the secretory pathway in real-time. Expression of this reporter protein did not itself elicit an ER stress response in cells; however, Gluc proved very sensitive at sensing this type of stress, which is associated with a temporary decrease in processing of proteins through the secretory pathway. The Gluc secretion assay was over 20,000-fold more sensitive as compared to the secreted alkaline phosphatase (SEAP), a well established assay for monitoring of protein processing and ER stress in mammalian cells. Conclusions/Significance: The Gluc assay provides a fast, quantitative and sensitive technique to monitor the secretory pathway and ER stress and its compatibility with high throughput screening will allow discovery of drugs for treatment of conditions in which the ER stress is generally induced

Effects of low intensity pulsed ultrasound with and without increased cortical porosity on structural bone allograft incorporation

<p>Abstract</p> <p>Background</p> <p>Though used for over a century, structural bone allografts suffer from a high rate of mechanical failure due to limited graft revitalization even after extended periods <it>in vivo</it>. Novel strategies that aim to improve graft incorporation are lacking but necessary to improve the long-term clinical outcome of patients receiving bone allografts. The current study evaluated the effect of low-intensity pulsed ultrasound (LIPUS), a potent exogenous biophysical stimulus used clinically to accelerate the course of fresh fracture healing, and longitudinal allograft perforations (LAP) as non-invasive therapies to improve revitalization of intercalary allografts in a sheep model.</p> <p>Methods</p> <p>Fifteen skeletally-mature ewes were assigned to five experimental groups based on allograft type and treatment: +CTL, -CTL, LIPUS, LAP, LIPUS+LAP. The +CTL animals (n = 3) received a tibial ostectomy with immediate replacement of the resected autologous graft. The -CTL group (n = 3) received fresh frozen ovine tibial allografts. The +CTL and -CTL groups did not receive LAP or LIPUS treatments. The LIPUS treatment group (n = 3), following grafting with fresh frozen ovine tibial allografts, received ultrasound stimulation for 20 minutes/day, 5 days/week, for the duration of the healing period. The LAP treatment group (n = 3) received fresh frozen ovine allografts with 500 μm longitudinal perforations that extended 10 mm into the graft. The LIPUS+LAP treatment group (n = 3) received both LIPUS and LAP interventions. All animals were humanely euthanized four months following graft transplantation for biomechanical and histological analysis.</p> <p>Results</p> <p>After four months of healing, daily LIPUS stimulation of the host-allograft junctions, alone or in combination with LAP, resulted in 30% increases in reconstruction stiffness, paralleled by significant increases (p < 0.001) in callus maturity and periosteal bridging across the host/allograft interfaces. Longitudinal perforations extending 10 mm into the proximal and distal endplates filled to varying degrees with new appositional bone and significantly accelerated revitalization of the allografts compared to controls.</p> <p>Conclusion</p> <p>The current study has demonstrated in a large animal model the potential of both LIPUS and LAP therapy to improve the degree of allograft incorporation. LAP may provide an option for increasing porosity, and thus potential <it>in vivo </it>osseous apposition and revitalization, without adversely affecting the structural integrity of the graft.</p

Trans-Species Polymorphism and Selection in the MHC Class II DRA Genes of Domestic Sheep

Highly polymorphic genes with central roles in lymphocyte mediated immune surveillance are grouped together in the major histocompatibility complex (MHC) in higher vertebrates. Generally, across vertebrate species the class II MHC DRA gene is highly conserved with only limited allelic variation. Here however, we provide evidence of trans-species polymorphism at the DRA locus in domestic sheep (Ovis aries). We describe variation at the Ovar-DRA locus that is far in excess of anything described in other vertebrate species. The divergent DRA allele (Ovar-DRA*0201) differs from the sheep reference sequences by 20 nucleotides, 12 of which appear non-synonymous. Furthermore, DRA*0201 is paired with an equally divergent DRB1 allele (Ovar-DRB1*0901), which is consistent with an independent evolutionary history for the DR sub-region within this MHC haplotype. No recombination was observed between the divergent DRA and B genes in a range of breeds and typical levels of MHC class II DR protein expression were detected at the surface of leukocyte populations obtained from animals homozygous for the DRA*0201, DRB1*0901 haplotype. Bayesian phylogenetic analysis groups Ovar-DRA*0201 with DRA sequences derived from species within the Oryx and Alcelaphus genera rather than clustering with other ovine and caprine DRA alleles. Tests for Darwinian selection identified 10 positively selected sites on the branch leading to Ovar-DRA*0201, three of which are predicted to be associated with the binding of peptide antigen. As the Ovis, Oryx and Alcelaphus genera have not shared a common ancestor for over 30 million years, the DRA*0201 and DRB1*0901 allelic pair is likely to be of ancient origin and present in the founding population from which all contemporary domestic sheep breeds are derived. The conservation of the integrity of this unusual DR allelic pair suggests some selective advantage which is likely to be associated with the presentation of pathogen antigen to T-cells and the induction of protective immunity

Response of Quercus ilex seedlings to Phytophthora spp. root infection in a soil infestation test

[EN] Phytophthora species are the main agents associated with oak (Quercus spp.) decline, together with the changing environmental conditions and the intensive land use. The aim of this study was to evaluate the susceptibility of Quercus ilex to the inoculation with eight Phytophthora species. Seven to eight month old Q. ilex seedlings grown from acorns, obtained from two Spanish origins, were inoculated with P. cinnamomi, P. cryptogea, P. gonapodyides, P. megasperma, P. nicotianae, P. plurivora, P. psychrophila and P. quercina. All Phytophthora inoculated seedlings showed decline and symptoms including small dark necrotic root lesions, root cankers, and loss of fine roots and tap root. The most aggressive species were P. cinnamomi, P. cryptogea, P. gonapodyides, P. plurivora and P. psychrophila followed by P. megasperma., while Phytophthora quercina and P. nicotianae were the less aggressive species. Results obtained confirm that these Phytophthora species could constituted a threat to Q. ilex ecosystems and the implications are further discussed.The authors are grateful to A. Solla and his team from the Centro Universitario de Plasencia-Universidad de Extremadura (Spain) for helping in the acorns collection and to the CIEF (Centro para la Investigación y Experimentación Forestal, Generalitat Valenciana, Valencia, Spain) for providing the acorns. This research was supported by funding from the project AGL2011- 30438-C02-01 (Ministerio de Economía y Competitividad, Spain).Mora-Sala, B.; Abad Campos, P.; Berbegal Martinez, M. (2018). Response of Quercus ilex seedlings to Phytophthora spp. root infection in a soil infestation test. European Journal of Plant Pathology. https://doi.org/10.1007/s10658-018-01650-6SÁlvarez, L. A., Pérez-Sierra, A., Armengol, J., & García-Jiménez, J. (2007). 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