Biochemistry and functional aspects of human glandular kallikreins

Human urinary kallikrein was purified by gel filtration on Sephacryl S-200 and affinity chromatography on aprotinin-Sepharose, followed by ion exchange chromatography on DEAE-Sepharose. In dodecylsulfate gel electrophoresis two protein bands with molecular weights of 41,000 and 34,000 were separated. The amino acid composition and the carbohydrate content of the kallikrein preparation were determined; isoleucine was identified as the only aminoterminal amino acid. The bimolecular velocity constant for the inhibition by diisopropyl fluorophosphate was determined as 9±2 l mol–1 min–1. The hydrolysis of a number of substrates was investigated and AcPheArgOEt was found to be the most sensitive substrate for human urinary kallikrein. Using this substrate an assay method for kallikrein in human urine was developed. It was shown by radioimmunoassay that pig pancreatic kallikrein can be absorbed in the rat intestinal tract. Furthermore, in dogs the renal excretion of glandular kallikrein from blood was demonstrated by radioimmunological methods

Comment on Neutron-Proton Spin-Correlation Parameter A_{ZZ} at 68 Mev

We present two arguments indicating that the large value for the $\epsilon_1$ mixing parameter at 50 MeV, which the Basel group extracted from their recent $A_{zz}$ measurement, may be incorrect. First, there are nucleon-nucleon (NN) potentials which predict the $\epsilon_1$ at 50 MeV substantially below the Basel value and reproduce the Basel $A_{zz}$ data accurately. Second, the large value for $\epsilon_1$ at 50 MeV proposed by the Basel group can only be explained by a model for the NN interaction which is very unrealistic (no $\rho$-meson and essentially a point-like $\pi NN$ vertex) and overpredicts the $\epsilon_1$ in the energy range where it is well determined (150--500 MeV) by a factor of two.Comment: 6 pages text (LaTex) and 2 figures (paper, will be faxed upon request), UI-NTH-930

Multi-mode mediated exchange coupling in cavity QED

Microwave cavities with high quality factors enable coherent coupling of distant quantum systems. Virtual photons lead to a transverse exchange interaction between qubits, when they are non-resonant with the cavity but resonant with each other. We experimentally probe the inverse scaling of the inter-qubit coupling with the detuning from a cavity mode and its proportionality to the qubit-cavity interaction strength. We demonstrate that the enhanced coupling at higher frequencies is mediated by multiple higher-harmonic cavity modes. Moreover, in the case of resonant qubits, the symmetry properties of the system lead to an allowed two-photon transition to the doubly excited qubit state and the formation of a dark state.Comment: 9 pages, 6 figure

Gamma-ray diagnostics of Type Ia supernovae: Predictions of observables from three-dimensional modeling

Besides the fact that the gamma-ray emission due to radioactive decays is responsible for powering the light curves of Type Ia supernovae (SNe Ia), gamma rays themselves are of particular interest as a diagnostic tool because they provide a direct way to obtain deeper insights into the nucleosynthesis and the kinematics of these explosion events. Focusing on two of the most broadly discussed SN Ia progenitor scenarios - a delayed detonation in a Chandrasekhar-mass white dwarf (WD) and a violent merger of two WDs - we use three-dimensional explosion models and perform radiative transfer simulations to obtain synthetic gamma-ray spectra. Both chosen models produce the same mass of 56Ni and have similar optical properties that are in reasonable agreement with the recently observed supernova SN 2011fe. In contrast to the optical regime, the gamma-ray emission of our two chosen models proves to be rather different. The almost direct connection of the emission of gamma rays to fundamental physical processes occuring in SNe Ia permits additional constraints concerning several explosion model properties that are not easily accessible within other wavelength ranges. Proposed future MeV missions such as GRIPS will resolve all spectral details only for nearby SNe Ia, but hardness ratio and light curve measurements still allow for a distinction of the two different models at 10 and 16 Mpc for an exposure time of 10^6 s, respectively. The possibility to detect the strongest line features up to the Virgo distance will offer the opportunity to build up a first sample of SN Ia detections in the gamma-ray energy range and underlines the importance of future space observatories for MeV gamma rays.Comment: 10 pages, 8 figures, accepted for publication by A&

Parametric Amplification of Nonlinear Response of Single Crystal Niobium

Giant enhancement of the nonlinear response of a single crystal Nb sample, placed in {\it a pumping ac magnetic field}, has been observed experimentally. The experimentally observed amplitude of the output signal is about three orders of magnitude higher than that seen without parametric pumping. The theoretical analysis based on the extended double well potential model provides a qualitative explanation of the experimental results as well as new predictions of two bifurcations for specific values of the pumping signal.Comment: 6 pages, 10 figure

Influence of magnetic field on paramagnetic-ferromagnetic transition in La1−x_{1-x}Cax_{x}MnO3_{3} (x≈0.25x\approx 0.25) crystal: ultrasonic and transport studies

The ultrasonic properties of La$_{1-x}$Ca$_{x}$MnO$_{3}$ ($x\approx 0.25$) with the Curie temperature $T_C$ about 200 K are studied. Temperature dependences of longitudinal and transverse sound velocities were measured in zero magnetic field and for different constant magnetic fields as well. The ultrasonic study is supported by magnetic, resistive, magnetoresistive, structural and other measurements of the sample that facilitate interpretation of the results obtained. The magnetic field influence on sound properties found in this study presents some new features of the interplay between the elastic and magnetic properties of these compounds. It is shown that the paramagnetic-ferromagnetic transition in the sample studied is first order, but can become second order under the influence of applied magnetic field.Comment: submitted to Phys. Rev.

Vortex Structure Around a Magnetic Dot in Planar Superconductors

The problem of the giant vortex state around a magnetic dot which is embedded in a superconducting film is investigated. The full non-linear, self-consistent Ginzburg-Landau equations are solved numerically in order to calculate the free energy, the order parameter of the host superconductor, the internal magnetic field due to the supercurrents, the corresponding current density, the magnetization probed in the vicinity of the dot, and the normal electron density as a function of the various parameters of the system. We find that, as we increase the magnetic moment of the dot, higher flux quanta vortex states become energetically more favorable, as they can better compete with the external magnetic field via the Meissner effect. In addition to that, they progressively become closer to each other in energy with direct experimental consequences, i.e. physical quantities like magnetization may fluctuate when measured, for example, as a function of a uniform external magnetic field.Comment: text 21 pages (REVTEX), 8 figures available upon reques

Nucleosynthesis in thermonuclear supernovae with tracers: convergence and variable mass particles

Nucleosynthetic yield predictions for multi-dimensional simulations of thermonuclear supernovae generally rely on the tracer particle method to obtain isotopic information of the ejected material for a given supernova simulation. We investigate how many tracer particles are required to determine converged integrated total nucleosynthetic yields. For this purpose, we conduct a resolution study in the number of tracer particles for different hydrodynamical explosion models at fixed spatial resolution. We perform hydrodynamic simulations on a co-expanding Eulerian grid in two dimensions assuming rotational symmetry for both pure deflagration and delayed detonation Type Ia supernova explosions. Within a given explosion model, we vary the number of tracer particles to determine the minimum needed for the method to give a robust prediction of the integrated yields of the most abundant nuclides. For the first time, we relax the usual assumption of constant tracer particle mass and introduce a radially vary- ing distribution of tracer particle masses. We find that the nucleosynthetic yields of the most abundant species (mass fraction > 10E-5) are reasonably well predicted for a tracer number as small as 32 per axis and direction - more or less independent of the explosion model. We conclude that the number of tracer particles that were used in extant published works appear to have been sufficient as far as integrated yields are concerned for the most copiously produced nuclides. Additionally we find that a suitably chosen tracer mass distribution can improve convergence for nuclei produced in the outer layer of the supernova where the constant tracer mass prescription suffers from poor spatial resolution.Comment: 9 pages, 5 figures, accepted for publication in MNRA

An upper limit on the contribution of accreting white dwarfs to the type Ia supernova rate

There is wide agreement that Type Ia supernovae (used as standard candles for cosmology) are associated with the thermonuclear explosions of white dwarf stars. The nuclear runaway that leads to the explosion could start in a white dwarf gradually accumulating matter from a companion star until it reaches the Chandrasekhar limit, or could be triggered by the merger of two white dwarfs in a compact binary system. The X-ray signatures of these two possible paths are very different. Whereas no strong electromagnetic emission is expected in the merger scenario until shortly before the supernova, the white dwarf accreting material from the normal star becomes a source of copious X-rays for ~1e7 yr before the explosion. This offers a means of determining which path dominates. Here we report that the observed X-ray flux from six nearby elliptical galaxies and galaxy bulges is a factor of ~30-50 less than predicted in the accretion scenario, based upon an estimate of the supernova rate from their K-band luminosities. We conclude that no more than ~5 per cent of Type Ia supernovae in early type galaxies can be produced by white dwarfs in accreting binary systems, unless their progenitors are much younger than the bulk of the stellar population in these galaxies, or explosions of sub-Chandrasekhar white dwarfs make a significant contribution to the supernova rate.Comment: 10 pages, 1 tabl