Foraminifers from the Lower-Middle Jurassic boundary of the Lusitanian Basin: Murtinheira section (North Sector) and Zambujal de Alcaria section (Central Sector)

Comunicação apresentada ao VIII Congresso Nacional de Geologia, em Braga (9-16 de Julho 2010)análise da diversidade taxonómica e das abundâncias relativas nas associações de foraminíferos da passagem Jurássico Inferior-Médio em dois sectores da Bacia Lusitânica indica que tais associações apresentam diferenças nas suas composições, aparentemente homogéneas. Assim, as associações de zonas proximais (perfil de Zambujal de Alcaria) são mais abundantes e com maior diversidade que as registadas em zonas distais (perfil da Murtinheira). Além disso, a maioria dos taxa comuns em ambas as zonas apresentam diferenças nas suas abundâncias relativas, que parecem resultar de preferências paleo-ambientais distintas.The analysis of diversity and relative abundances of foraminiferal assemblages from two sections located in the Lusitanian Basin, and corresponding to the Lower-Middle Jurassic boundary, shows that these apparently homogeneous assemblages present differences in their composition. Assemblages from shallow zones (Zambujal de Alcaria section) show higher diversity and abundance than assemblages from deeper zones (Murtinheira section). Species recorded in both areas show distinct values on their relative abundances, as a possible relationship with their palaeo-environmental preferences

Mapping key concepts of e-learning and educational-systematic review through published papers

E-Learning also known as electronic learning has gained a wide acceptance from all educational fields and academic institutions. In the past decades we have experienced a profound growth of e-learning courses in education and multiple systems have appeared from medicine to management covering different needs and users. Previous studies indicate a proliferation and discrepancy of e-learning contents that affect interoperability patterns in education for students, teachers and academic staff and have done little to assess the usability of e-learning educational systems leading to contradictory findings that can mislead academics and users.info:eu-repo/semantics/acceptedVersio

Tracking e-learning through published papers: a systematic review

Electronic learning (e-learning) is a broader approach to learning that brings new opportunities for learning and teaching in many fields of education far from the traditional classroom environment. Over the past decades, research in the field indicates a proliferation of e-learning contents and discrepancies that affect interoperability patterns in education for students and teachers; however, little has been done to assess the usability of e-learning systems. From a different perspective, this study aims to provide information on the numerous findings relating to the cumulative results of e-learning in education. This systematic review uses a full protocol with the aim of standardizing and specifying all the procedures adopted to collect and code 99 academic articles from 2010 to 2018 with keywords: education and e-learning. The text analysis as conducted using the qualitative software Leximancer to extract meaning from the large number of articles retrieved. The results highlight four dominant themes, namely education systems and learning issues that in turn promote student behaviours and the use of online learning tools. This research contributes towards providing research propositions that can be used in a cogent theoretical framework and, based on the analysis, we also propose a new definition of e-learning.info:eu-repo/semantics/acceptedVersio

Alimentos e alimentação de galinhas poedeiras em sistemas orgânicos de produção.

bitstream/item/57839/1/CUsersPiazzonDocumentsCIT-55.pdfProjeto/Plano de Ação: 16.00.30.004-00

Isolation Method and Characterization of Outer Membranes Vesicles of Helicobacter pylori Grown in a Chemically Defined Medium

Outer membrane vesicles (OMVs) are small vesicles constitutively shed by all Gram-negative bacterium, which have been proposed to play a role in Helicobacter pylori persistence and pathogenesis. The methods currently available for the isolation of H. pylori OMVs are diverse and time-consuming, raising the need for a protocol standardization, which was the main aim of this study. Here, we showed that the chemically defined F12 medium, supplemented with cholesterol, nutritionally supports bacterial growth and maintains H. pylori viability for at least 72 h. Additionally, we developed an abridged protocol for isolation of OMVs from these bacterial cultures, which comprises a low-speed centrifugation, supernatant filtration through a 0.45 µm pore, and two ultracentrifugations for OMVs’ recovery and washing. Using this approach, a good yield of highly pure bona fide OMVs was recovered from cultures of different H. pylori strains and in different periods of bacterial growth, as assessed by nanoparticle tracking analysis, transmission electron microscopy (TEM), and proteomic analyses, confirming the reliability of the protocol. Analysis of the proteome of OMVs isolated from H. pylori F12-cholesterol cultures at different time points of bacterial growth revealed differentially expressed proteins, including the vacuolating cytotoxin VacA. In conclusion, this work proposes a time- and cost-efficient protocol for the isolation of H. pylori OMVs from a chemically defined culture medium that is suitable for implementation in research and in the biopharmaceutical field.This article is a result of the project NORTE-01-0145-FEDER-000029, supported by Norte Portugal Regional Program (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). The i3S HEMS Scientific Platform is member of the national infrastructure PPBI – Portuguese Platform of Bioimaging (PPBI-POCI-01-0145-FEDER-022122). The i3S Proteomics Scientific Platform is funded by the Portuguese Mass Spectrometry Network, integrated in the National Roadmap of Research Infrastructures of Strategic Relevance (ROTEIRO/0028/2013; LISBOA-01-0145-FEDER-022125). JM and ML had fellowships from FCT – Fundação para a Ciência e a Tecnologia (SFRH/BD/116965/2016 and SFRH/BDP/110065/2015). ML has a FCT RJEC Id 3762 contract

Basin structure in the two-dimensional dissipative circle map

Fractal basin structure in the two-dimensional dissipative circle map is examined in detail. Numerically obtained basin appears to be riddling in the parameter region where two periodic orbits co-exist near a boundary crisis, but it is shown to consist of layers of thin bands.Comment: published in J. Phys. Soc. Jpn., 72, 1943-1947 (2003

Renal trauma: What changed in the past decade

info:eu-repo/semantics/acceptedVersio

Small bowel pseudomelanosis and oral iron therapy

Small bowel pseudomelanosis is a rarely reported clinical entity characterized by brown pigmentation of small bowel mucosa. The authors describe two cases, both with iron deficiency anemia, one of an 81-year-old female patient submitted for capsule endoscopy that revealed a brown pigmentation of all small bowel mucosa and another of an 81-year-old male whose retrograde double-balloon enteroscopy revealed a diffuse brown pattern of small bowel mucosa. Ileal biopsies confirmed intense iron deposition in the macrophages of the lamina propria. Both patients were on oral iron therapy and the second one had a previous double-balloon enteroscopy, 2 years earlier, which revealed only ileal angiodysplasias. These two cases demonstrate the importance of two new endoscopic methods for diagnosis of small bowel pseudomelanosis, the rarity of such an entity and its close relation with oral iron therapy