Rocket-triggered lightning strikes and forest fire ignition

Background information on the rocket-triggered lightning project at Kennedy Space Center (KSC), a summary of the forecasting problem there, the facilities and equipment available for undertaking field experiments at KSC, previous research activity performed, a description of the atmospheric science field laboratory near Mosquito Lagoon on the KSC complex, methods of data acquisition, and present results are discussed. New sources of data for the 1989 field experiment include measuring the electric field in the lower few thousand feet of the atmosphere by suspending field measuring devices below a tethered balloon. Problems encountered during the 1989 field experiment are discussed. Future prospects for both triggered lightning and lightning-kindled forest fire research at KSC are listed

Fractional Chern Insulators in Bands with Zero Berry Curvature

Even if a noninteracting system has zero Berry curvature everywhere in the Brillouin zone, it is possible to introduce interactions that stabilise a fractional Chern insulator. These interactions necessarily break time-reversal symmetry (either spontaneously or explicitly) and have the effect of altering the underlying band structure. We outline a number of ways in which this may be achieved, and show how similar interactions may also be used to create a (time-reversal symmetric) fractional topological insulator. While our approach is rigorous in the limit of long range interactions, we show numerically that even for short range interactions a fractional Chern insulator can be stabilised in a band with zero Berry curvature.Comment: 7 pages, 2 figures; Published versio

Perturbative Approach to Flat Chern Bands in the Hofstadter Model

We present a perturbative approach to the study of the Hofstadter model for when the amount of flux per plaquette is close to a rational fraction. Within this approximation certain eigenstates of the system are shown to be multi-component wavefunctions that connect smoothly to the Landau levels of the continuum. The perturbative corrections to these are higher Landau level contributions that break rotational invariance and allow the perturbed states to adopt the symmetry of the lattice. In the presence of interactions, this approach allows for the calculation of generalised Haldane pseudopotentials, and in turn, the many-body properties of the system. The method is sufficiently general that it can apply to a wide variety of lattices, interactions and magnetic field strengths.Comment: 40 pages, 15 figures; v2 includes minor changes, additional references and an expanded background sectio

The biologic digestion of garbage with sewage sludge

Bibliography: p. 105-109

A multifrequency evaluation of active and passive microwave sensors for oil spill detection and assessment

An evaluation is given of how active and passive microwave sensors can best be used in oil spill detection and assessment. Radar backscatter curves taken over oil spills are presented and their effect on synthetic aperture radar (SAR) imagery are discussed. Plots of microwave radiometric brightness variations over oil spills are presented and discussed. Recommendations as to how to select the best combination of frequency, viewing angle, and sensor type for evaluation of various aspects of oil spills are also discussed

A stochastic model for the evolution of the web allowing link deletion

Recently several authors have proposed stochastic evolutionary models for the growth of the web graph and other networks that give rise to power-law distributions. These models are based on the notion of preferential attachment leading to the ``rich get richer'' phenomenon. We present a generalisation of the basic model by allowing deletion of individual links and show that it also gives rise to a power-law distribution. We derive the mean-field equations for this stochastic model and show that by examining a snapshot of the distribution at the steady state of the model, we are able to tell whether any link deletion has taken place and estimate the link deletion probability. Our model enables us to gain some insight into the distribution of inlinks in the web graph, in particular it suggests a power-law exponent of approximately 2.15 rather than the widely published exponent of 2.1

The Galactic Habitable Zone and the Age Distribution of Complex Life in the Milky Way

We modeled the evolution of the Milky Way to trace the distribution in space and time of four prerequisites for complex life: the presence of a host star, enough heavy elements to form terrestrial planets, sufficient time for biological evolution and an environment free of life-extinguishing supernovae. We identified the Galactic habitable zone (GHZ) as an annular region between 7 and 9 kiloparsecs from the Galactic center that widens with time and is composed of stars that formed between 8 and 4 billion years ago. This GHZ yields an age distribution for the complex life that may inhabit our Galaxy. We found that 75% of the stars in the GHZ are older than the Sun.Comment: 9 pages, 4 figs. Published in Science, 2 January 200

Identification of polyubiquitin binding proteins involved in NF-kappaB signaling using protein arrays.

Attachment of ubiquitin to proteins represents a central mechanism for the regulation of protein metabolism and function. In the NF-kappaB pathway, binding of NEMO to polyubiquitinated substrates initiates the pathway in response to cellular stimuli. Other polyubiquitin binding proteins can antagonize this pathway by competing with NEMO for polyubiquitin. We have used protein arrays to identify polyubiquitin binding proteins that regulate NF-kappaB activity. Using polyubiquitin as bait, protein arrays were screened and polyubiquitin binders identified. Novel polyubiquitin binders AWP1, CALCOCO2, N4BP1, RIO3, TEX27, TTC3, UBFD1 and ZNF313 were identified using this approach, while known NF-kappaB regulators including NEMO, A20, ABIN-1, ABIN-2, optineurin and p62 were also identified. Overexpressed AWP1 and RIO3 repressed NF-kappaB activity in a manner similar to optineurin, while siRNAs directed against AWP1 and RIO3 also reduced NF-kappaB activity. TNFalpha-dependent degradation of IkappaBalpha was also suppressed by overexpression of AWP1 and RIO3, possibly due to the polyubiquitin binding activity of these proteins. Protein array screening using polyubiquitin enabled rapid identification of many known and novel polyubiquitin binding proteins and the identification of novel NF-kappaB regulators

Expanding the Substantial Interactome of NEMO Using Protein Microarrays

Signal transduction by the NF-kappaB pathway is a key regulator of a host of cellular responses to extracellular and intracellular messages. The NEMO adaptor protein lies at the top of this pathway and serves as a molecular conduit, connecting signals transmitted from upstream sensors to the downstream NF-kappaB transcription factor and subsequent gene activation. The position of NEMO within this pathway makes it an attractive target from which to search for new proteins that link NF-kappaB signaling to additional pathways and upstream effectors. In this work, we have used protein microarrays to identify novel NEMO interactors. A total of 112 protein interactors were identified, with the most statistically significant hit being the canonical NEMO interactor IKKbeta, with IKKalpha also being identified. Of the novel interactors, more than 30% were kinases, while at least 25% were involved in signal transduction. Binding of NEMO to several interactors, including CALB1, CDK2, SAG, SENP2 and SYT1, was confirmed using GST pulldown assays and coimmunoprecipitation, validating the initial screening approach. Overexpression of CALB1, CDK2 and SAG was found to stimulate transcriptional activation by NF-kappaB, while SYT1 overexpression repressed TNFalpha-dependent NF-kappaB transcriptional activation in human embryonic kidney cells. Corresponding with this finding, RNA silencing of CDK2, SAG and SENP2 reduced NF-kappaB transcriptional activation, supporting a positive role for these proteins in the NF-kappaB pathway. The identification of a host of new NEMO interactors opens up new research opportunities to improve understanding of this essential cell signaling pathway