Atorvastatin correlates with decreased risk of esophageal cancer: A population-based casecontrol study from Taiwan

Objectives: The aim of this study was to explore the association between the use of statins and esophageal cancer in Taiwan.Methods: We designed a casecontrol study using database from the Taiwan National Health Insurance program. In all, 549 patients (cases) aged 20 years or older diagnosed recently with esophageal cancer, from 2000 to 2009, and 2,196 subjects (controls) without esophageal cancer participated in this study. The association between esophageal cancer and the use of statins and other co-morbidities was measured.Results: After adjustment for covariates, multivariate logistic regression showed that patients with a cumulative duration of ]12 months of using atorvastatin might have a reduced risk of esophageal cancer, compared with those who did not use statins (odds ratio [OR] 0.14, 95% confidence interval [CI] 0.040.56). The other statins could not show a significant association with esophageal cancer. Age (OR 1.01, 95% CI 1.001.01), alcoholism (OR 3.83, 95% CI 3.014.89), and esophageal diseases (OR 4.60, 95% CI 3.466.12) were independent factors significantly associated with esophageal cancer.Conclusions: Use of atorvastatin ]12 months may correlate with an 86% reduction of esophageal cancer risk.Keywords: atorvastatin; esophageal cancer; stati

Atorvastatin correlates with decreased risk of esophageal cancer: A population-based casecontrol study from Taiwan

Objectives: The aim of this study was to explore the association between the use of statins and esophageal cancer in Taiwan.Methods: We designed a casecontrol study using database from the Taiwan National Health Insurance program. In all, 549 patients (cases) aged 20 years or older diagnosed recently with esophageal cancer, from 2000 to 2009, and 2,196 subjects (controls) without esophageal cancer participated in this study. The association between esophageal cancer and the use of statins and other co-morbidities was measured.Results: After adjustment for covariates, multivariate logistic regression showed that patients with a cumulative duration of ]12 months of using atorvastatin might have a reduced risk of esophageal cancer, compared with those who did not use statins (odds ratio [OR] 0.14, 95% confidence interval [CI] 0.040.56). The other statins could not show a significant association with esophageal cancer. Age (OR 1.01, 95% CI 1.001.01), alcoholism (OR 3.83, 95% CI 3.014.89), and esophageal diseases (OR 4.60, 95% CI 3.466.12) were independent factors significantly associated with esophageal cancer.Conclusions: Use of atorvastatin ]12 months may correlate with an 86% reduction of esophageal cancer risk.Keywords: atorvastatin; esophageal cancer; stati

Strength and Durability Performance of Ultra-High-Performance Cementitious Composite Enhanced with Carbon Nanofibres

Ultra-high-performance concrete (UHPC) is known for exhibiting excellent strength and a more durable matrix compared to conventional concrete. Typically UHPC consists of carefully selected constituent materials of: ultrafine graded sand, silica fume (or other alternative binders), steel micro-fibres, and ordinary (or special blends) Portland cement. This study was initiated to synthesize a new blend of Ultra-high-performance cementitious composite (UHPCC) with compressive strength higher than 150 MPa under normal curing conditions, and to investigate the influence of two different sources of carbon nanofibres (CNF) on its strength, durability and microstructure properties. Several mix designs with different CNF percentages were designed, optimised and analysed to obtain the optimal proportion for the UHPCC, and their strength development were monitored up to 28 days. Subsequently, the durability performance of the selected UHPCC mixes were characterised though the rapid chloride permeability, Mercury Intrusion Porosimetry and water penetration tests. It was found that a stable dispersion with low CNF percentage (0.06%wt) was able to improve the water penetration, lower the rapid chloride permeability and reduced the pore sizes of the UHPCC matrix. The overall findings of the research assert that a stable dispersion of CNF contributes in positive effect on the strength and durability characteristics of UHPCC, and is feasible to enhance overall microstructure and contribute to a denser matrix. This study is part of the larger research programme to synthesize an innovative UHPCC mix for specialised applications for structures under impulsive loadings

Reconstitution of bone-like matrix in osteogenically differentiated mesenchymal stem cells-collagen constructs: a three-dimensional in vitro model to study hematopoietic stem cell niche

published_or_final_versio

Flexural behavior of Steel-Fiber-Added-RC (SFARC) beams with C30 and C50 classes of concrete

Although conventional reinforced concrete (RC) is the most globally used building material; however, its detrimental structural characteristics such as brittle failure mechanism in tension need to be improved. Discrete and short steel fibers (SFs) can be added into the concrete mix to improve its brittleness. The effects of the addition of optimum percentage of SFs on flexural behavior of RC beams have been investigated in this paper. In this study, the optimum percentage of hooked-end SFs with the dimensions of 0.75mm in diameter and 50mm in length are added in RC beams with two different classes of concrete (i.e. two different compressive strengths of 30MPa (C30) and 50MPa (C50)). In order to determine the optimum percentage of SFs added to the concrete mix, 5 prisms and 30 cubes with 5 different percentages of SFs (i.e. 0%v/v, 0.5%v/v, 1%v/v, 1.5%v/v, and 2%v/v) from both C30 and C50 classes of concrete have been tested. Based on the results of the flexural strength and compressive strength tests, it is found that the optimum value is 1% of SFs by volume (i.e. 78.5 kg/m3). To investigate the flexural behavior of steel fiber added RC (SFARC) beams compared to conventional RC beams with no SFs, two RC beams with the dimensions of 170 mm in height, 120mm in width, and 2400mm in length, with SF percentages of 0 and 1%v/v and both having exactly the same steel reinforcement were tested under flexure using a four-point loading test setup for both C30 and C50 classes of concrete. The experimental results show that the SFARC beams with 1.0% by volume of SFs have higher first cracking strength, ultimate flexural strength, stiffness, and ductility compared to those conventional RC beams with no SFs. Furthermore, the addition of SFs has more effects on RC beams with higher compressive strength (50MPa) compared to lower concrete grade (30MPa)

The speciation and genotyping of Cronobacter isolates from hospitalised patients

The World Health Organization (WHO) has recognised all Cronobacter species as human pathogens. Among premature neonates and immunocompromised infants, these infections can be life-threatening, with clinical presentations of septicaemia, meningitis and necrotising enterocolitis. The neurological sequelae can be permanent and the mortality rate as high as 40 – 80 %. Despite the highlighted issues of neonatal infections, the majority of Cronobacter infections are in the elderly population suffering from serious underlying disease or malignancy and include wound and urinary tract infections, osteomyelitis, bacteraemia and septicaemia. However, no age profiling studies have speciated or genotyped the Cronobacter isolates. A clinical collection of 51 Cronobacter strains from two hospitals were speciated and genotyped using 7-loci multilocus sequence typing (MLST), rpoB gene sequence analysis, O-antigen typing and pulsed- field gel electrophoresis (PFGE). The isolates were predominated by C. sakazakii sequence type 4 (63 %, 32/51) and C. malonaticus sequence type 7 (33 %, 17/51). These had been isolated from throat and sputum samples of all age groups, as well as recal and faecal swabs. There was no apparent relatedness between the age of the patient and the Cronobacter species isolated. Despite the high clonality of Cronobacter , PFGE profiles differentiated strains across the sequence types into 15 pulsotypes. There was almost complete agreement between O-antigen typing and rpoB gene sequence analysis and MLST profiling. This study shows the value of applying MLST to bacterial population studies with strains from two patient cohorts, combined with PFGE for further discrimination of strains

Large-scale plasma proteomic profiling identifies a high-performance biomarker panel for Alzheimer's disease screening and staging

INTRODUCTION: Blood proteins are emerging as candidate biomarkers for Alzheimer's disease (AD). We systematically profiled the plasma proteome to identify novel AD blood biomarkers and develop a high-performance, blood-based test for AD. METHODS: We quantified 1160 plasma proteins in a Hong Kong Chinese cohort by high-throughput proximity extension assay and validated the results in an independent cohort. In subgroup analyses, plasma biomarkers for amyloid, tau, phosphorylated tau, and neurodegeneration were used as endophenotypes of AD. RESULTS: We identified 429 proteins that were dysregulated in AD plasma. We selected 19 “hub proteins” representative of the AD plasma protein profile, which formed the basis of a scoring system that accurately classified clinical AD (area under the curve = 0.9690–0.9816) and associated endophenotypes. Moreover, specific hub proteins exhibit disease stage-dependent dysregulation, which can delineate AD stages. DISCUSSION: This study comprehensively profiled the AD plasma proteome and serves as a foundation for a high-performance, blood-based test for clinical AD screening and staging

Cardiovascular sequalae in uncomplicated COVID-19 survivors

BACKGROUND: A high proportion of COVID-19 patients were reported to have cardiac involvements. Data pertaining to cardiac sequalae is of urgent importance to define subsequent cardiac surveillance. METHODS: We performed a systematic cardiac screening for 97 consecutive COVID-19 survivors including electrocardiogram (ECG), echocardiography, serum troponin and NT-proBNP assay 1-4 weeks after hospital discharge. Treadmill exercise test and cardiac magnetic resonance imaging (CMR) were performed according to initial screening results. RESULTS: The mean age was 46.5 ± 18.6 years; 53.6% were men. All were classified with non-severe disease without overt cardiac manifestations and did not require intensive care. Median hospitalization stay was 17 days and median duration from discharge to screening was 11 days. Cardiac abnormalities were detected in 42.3% including sinus bradycardia (29.9%), newly detected T-wave abnormality (8.2%), elevated troponin level (6.2%), newly detected atrial fibrillation (1.0%), and newly detected left ventricular systolic dysfunction with elevated NT-proBNP level (1.0%). Significant sinus bradycardia with heart rate below 50 bpm was detected in 7.2% COVID-19 survivors, which appeared to be self-limiting and recovered over time. For COVID-19 survivors with persistent elevation of troponin level after discharge or newly detected T wave abnormality, echocardiography and CMR did not reveal any evidence of infarct, myocarditis, or left ventricular systolic dysfunction. CONCLUSION: Cardiac abnormality is common amongst COVID-survivors with mild disease, which is mostly self-limiting. Nonetheless, cardiac surveillance in form of ECG and/or serum biomarkers may be advisable to detect more severe cardiac involvement including atrial fibrillation and left ventricular dysfunction

Response of Sunflower (Helianthus annuus L.) Leaf Surface Defenses to Exogenous Methyl Jasmonate

Helianthus annuus, the common sunflower, produces a complex array of secondary compounds that are secreted into glandular trichomes, specialized structures found on leaf surfaces and anther appendages of flowers. The primary components of these trichome secretions are sesquiterpene lactones (STL), a diverse class of compounds produced abundantly by the plant family Compositae and believed to contribute to plant defense against herbivory. We treated wild and cultivated H. annuus accessions with exogenous methyl jasmonate, a plant hormone that mediates plant defense against insect herbivores and certain classes of fungal pathogens. The wild sunflower produced a higher density of glandular trichomes on its leaves than the cultivar. Comparison of the profiles of glandular trichome extracts obtained by liquid chromatography–mass spectroscopy (LC-MS) showed that wild and cultivated H. annuus were qualitatively similar in surface chemistry, although differing in the relative size and proportion of various compounds detected. Despite observing consistent transcriptional responses to methyl jasmonate treatment, we detected no significant effect on glandular trichome density or LC-MS profile in cultivated or wild sunflower, with wild sunflower exhibiting a declining trend in overall STL production and foliar glandular trichome density of jasmonate-treated plants. These results suggest that glandular trichomes and associated compounds may act as constitutive defenses or require greater levels of stimulus for induction than the observed transcriptional responses to exogenous jasmonate. Reduced defense investment in domesticated lines is consistent with predicted tradeoffs caused by selection for increased yield; future research will focus on the development of genetic resources to explicitly test the ecological roles of glandular trichomes and associated effects on plant growth and fitness

Tnni3k Modifies Disease Progression in Murine Models of Cardiomyopathy

The Calsequestrin (Csq) transgenic mouse model of cardiomyopathy exhibits wide variation in phenotypic progression dependent on genetic background. Seven heart failure modifier (Hrtfm) loci modify disease progression and outcome. Here we report Tnni3k (cardiac Troponin I-interacting kinase) as the gene underlying Hrtfm2. Strains with the more susceptible phenotype exhibit high transcript levels while less susceptible strains show dramatically reduced transcript levels. This decrease is caused by an intronic SNP in low-transcript strains that activates a cryptic splice site leading to a frameshifted transcript, followed by nonsense-mediated decay of message and an absence of detectable protein. A transgenic animal overexpressing human TNNI3K alone exhibits no cardiac phenotype. However, TNNI3K/Csq double transgenics display severely impaired systolic function and reduced survival, indicating that TNNI3K expression modifies disease progression. TNNI3K expression also accelerates disease progression in a pressure-overload model of heart failure. These combined data demonstrate that Tnni3k plays a critical role in the modulation of different forms of heart disease, and this protein may provide a novel target for therapeutic intervention