Proteomic characterization of murid herpesvirus 4 extracellular virions.

Gammaherpesvirinae, such as the human Epstein-Barr virus (EBV) and the Kaposi's sarcoma associated herpesvirus (KSHV) are highly prevalent pathogens that have been associated with several neoplastic diseases. As EBV and KSHV are host-range specific and replicate poorly in vitro, animal counterparts such as Murid herpesvirus-4 (MuHV-4) have been widely used as models. In this study, we used MuHV-4 in order to improve the knowledge about proteins that compose gammaherpesviruses virions. To this end, MuHV-4 extracellular virions were isolated and structural proteins were identified using liquid chromatography tandem mass spectrometry-based proteomic approaches. These analyses allowed the identification of 31 structural proteins encoded by the MuHV-4 genome which were classified as capsid (8), envelope (9), tegument (13) and unclassified (1) structural proteins. In addition, we estimated the relative abundance of the identified proteins in MuHV-4 virions by using exponentially modified protein abundance index analyses. In parallel, several host proteins were found in purified MuHV-4 virions including Annexin A2. Although Annexin A2 has previously been detected in different virions from various families, its role in the virion remains controversial. Interestingly, despite its relatively high abundance in virions, Annexin A2 was not essential for the growth of MuHV-4 in vitro. Altogether, these results extend previous work aimed at determining the composition of gammaherpesvirus virions and provide novel insights for understanding MuHV-4 biology

Sujets clés pour les barrières d'installations de stockage de déchets

International audienceThe objective of the paper is to give an update in key topics related to performance issues of barrier systems for landfills. The objective of using barrier systems is to minimize the impact of contaminants on the surrounding environment. To achieve this goal puncture protection of the geomembrane must be ensured. An update is first given is this matter. The question of the stability on slope of geosynthetic barrier systems is then discussed and an insight is given in modeling and laboratory measurement of parameters required to perform reliable modeling, especially as regards the case of piggy-back landfills. Finally, the question of transfers though bottom barrier systems is addressed, giving an update especially in the analytical solutions developed in the past 10 years in China in this matter