3,044 research outputs found
A compact and efficient strontium oven for laser-cooling experiments
Here we describe a compact and efficient strontium oven well suited for
laser-cooling experiments. Novel design solutions allowed us to produce a
collimated strontium atomic beam with a flux of 1.0\times10^13 s^-1 cm^-2 at
the oven temperature of 450 {\deg}C, reached with an electrical power
consumption of 36 W. The oven is based on a stainless-steel reservoir, filled
with 6 g of metallic strontium, electrically heated in a vacuum environment by
a tantalum wire threaded through an alumina multi-bore tube. The oven can be
hosted in a standard DN40CF cube and has an estimated continuous operation
lifetime of 10 years. This oven can be used for other alkali and alkaline earth
metals with essentially no modifications.Comment: 6 pages, 6 figures, Review of Scientific Instruments, in pres
Synthetic protein–protein interaction domains created by shuffling Cys(2)His(2) zinc-fingers
Cys(2)His(2) zinc-fingers (C2H2 ZFs) mediate a wide variety of protein–DNA and protein–protein interactions. DNA-binding C2H2 ZFs can be shuffled to yield artificial proteins with different DNA-binding specificities. Here we demonstrate that shuffling of C2H2 ZFs from transcription factor dimerization zinc-finger (DZF) domains can also yield two-finger DZFs with novel protein–protein interaction specificities. We show that these synthetic protein–protein interaction domains can be used to mediate activation of a single-copy reporter gene in bacterial cells and of an endogenous gene in human cells. In addition, the synthetic two-finger domains we constructed can also be linked together to create more extended, four-finger interfaces. Our results demonstrate that shuffling of C2H2 ZFs can yield artificial protein-interaction components that should be useful for applications in synthetic biology
Groundwater declines are linked to changes in Great Plains stream fish assemblages
Groundwater pumping for agriculture is a major driver causing declines of global freshwater ecosystems, yet the ecological consequences for stream fish assemblages are rarely quantified. We combined retrospective (1950–2010) and prospective (2011–2060) modeling approaches within a multiscale framework to predict change in Great Plains stream fish assemblages associated with groundwater pumping from the United States High Plains Aquifer. We modeled the relationship between the length of stream receiving water from the High Plains Aquifer and the occurrence of fishes characteristic of small and large streams in the western Great Plains at a regional scale and for six subwatersheds nested within the region. Water development at the regional scale was associated with construction of 154 barriers that fragment stream habitats, increased depth to groundwater and loss of 558 km of stream, and transformation of fish assemblage structure from dominance by large-stream to small-stream fishes. Scaling down to subwatersheds revealed consistent transformations in fish assemblage structure among western subwatersheds with increasing depths to groundwater. Although transformations occurred in the absence of barriers, barriers along mainstem rivers isolate depauperate western fish assemblages from relatively intact eastern fish assemblages. Projections to 2060 indicate loss of an additional 286 km of stream across the region, as well as continued replacement of largestream fishes by small-stream fishes where groundwater pumping has increased depth to groundwater. Our work illustrates the shrinking of streams and homogenization of Great Plains stream fish assemblages related to groundwater pumping, and we predict similar transformations worldwide where local and regional aquifer depletions occur
Quantum coherence controls the charge separation in a prototypical artificial light harvesting system
In artificial light harvesting systems the conversion of light into charges or chemical energy happens on the femtosecond time scale and is thought to involve the incoherent jump of an electron from the optical absorber to an electron acceptor. Here we investigate the primary process of electronic charge transfer dynamics in a carotene-porphyrin-fullerene triad, a prototypical elementary component for an artificial light harvesting system combining coherent femtosecond spectroscopy and first-principles quantum dynamics simulations. Our experimental and theoretical results provide strong evidence that the driving mechanism of the photoinduced current generation cycle is a quantum-correlated wavelike motion of electrons and nuclei on a timescale of few tens of femtoseconds. We furthermore highlight the fundamental role played by the interface between the light-absorbing chromophore and the charge acceptor in triggering the coherent wavelike electron-hole splitting. © 2013 IEEE
The Space Optical Clocks Project: Development of high-performance transportable and breadboard optical clocks and advanced subsystems
The use of ultra-precise optical clocks in space ("master clocks") will allow
for a range of new applications in the fields of fundamental physics (tests of
Einstein's theory of General Relativity, time and frequency metrology by means
of the comparison of distant terrestrial clocks), geophysics (mapping of the
gravitational potential of Earth), and astronomy (providing local oscillators
for radio ranging and interferometry in space). Within the ELIPS-3 program of
ESA, the "Space Optical Clocks" (SOC) project aims to install and to operate an
optical lattice clock on the ISS towards the end of this decade, as a natural
follow-on to the ACES mission, improving its performance by at least one order
of magnitude. The payload is planned to include an optical lattice clock, as
well as a frequency comb, a microwave link, and an optical link for comparisons
of the ISS clock with ground clocks located in several countries and
continents. Undertaking a necessary step towards optical clocks in space, the
EU-FP7-SPACE-2010-1 project no. 263500 (SOC2) (2011-2015) aims at two
"engineering confidence", accurate transportable lattice optical clock
demonstrators having relative frequency instability below 1\times10^-15 at 1 s
integration time and relative inaccuracy below 5\times10^-17. This goal
performance is about 2 and 1 orders better in instability and inaccuracy,
respectively, than today's best transportable clocks. The devices will be based
on trapped neutral ytterbium and strontium atoms. One device will be a
breadboard. The two systems will be validated in laboratory environments and
their performance will be established by comparison with laboratory optical
clocks and primary frequency standards. In this paper we present the project
and the results achieved during the first year.Comment: Contribution to European Frequency and Time Forum 2012, Gothenburg,
Swede
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