The importance of hematocrit for oxygen delivery and hemodynamics

Anemia is common in elderly patients undergoing surgery and in critical patients. A 72-year-old man submitted to a revision of hip replacement implant was diagnosed with tuberculosis, followed by pulmonary fibrosis, pulmonary heart disease and compensatory erythrocytosis. In the postoperative period, he got anemia which improved his clinical status. Anemia reduces viscosity, i.e. one of the components of vascular resistance to laminar (according to the law of Hagen-Poiseuille) and turbulent flows. In conditions of decreased hematocrit, shear thinning occurs more easily and in larger caliber vessels. Hemodiluition reduces both right and left cardiac afterloads, thus provoking an improvement of the blood flow. As the hematocrit decreases, oxygen delivery increases, because the increase in the cardiac output is greater than the decrease in the concentration of hemoglobin. Further studies are needed to confirm this physical model and to establish the variable and degree of the transfusion trigger

METHODS FOR OBTAINING. HOLLOW NANO-STRUCTURES

Methods are provided for obtaining hollow nano-structures which include the steps of providing a Suspended film starting layer on a Support Substrate, depositing on the starting layer a sacrificial layer, performing, in progressive sequence, a com plete erosion phase of said Support Substrate and starting layer and performing an at least partial erosion phase of the sacri ficial layer previously deposited on the starting layer so as to obtain holes passing through the starting layer and passing or non passing through the sacrificial layer, depositing, on the side of the support substrate opposite to that where the start ing layer is put, at least one covering layer arranged to inter nally cover the holes created by the progressive erosion. Hol low nano-structures formed by Such methods are also provided

Oral hygiene management in patients with visual sensory disabilities

Aim: Oral hygiene maintenance is one of the most difficult tasks for visually impaired people. The aim of study was to investigate about knowledge on oral hygiene practices among patients with visual sensory disabilities by proposing an effective management in order to achieve and maintain oral health status of these patients. Methods:It was administered a questionnaire about oral health management to the patients with visual disabilities accessing to dental unit of “Mons. Di Liegro” Hospital of Gaeta. Results: The survey covered a sample of 49 patients, aged between 14 and 95 years. More than half (66%) was blind ( 65% of cases with primary blindness and the remain ing 35% with secondary blindness). Only 32.65% brushed their teeth 3 times a day; 68% of the surveyed patients limited home oral hygiene procedures to toothbrush and toothpaste; 79% used manual toothbrush; 49% of respondents report ed odontophobia (it was basically generated by pain) often due to bad experience during childhood. More than half declared a dental office attendance as needed. Conclusions: This study showed as, although starting from a compromised oral health and inadequate knowledge of oral hygiene practices, visual impaired/ blind patients were able to achieve and maintain a good level of oral hygiene, using the most appropriate techniques and instrument

Mapping the local dielectric response at the nanoscale by means of plasmonic force spectroscopy

At the present, the local optical properties of nanostructured materials are difficult to be measured by available instrumentation. We investigated the capability of plasmonic force spectroscopy of measuring the optical response at the nanoscale. The proposed technique is based on force measurements performed by combining Atomic Force Microscopy, or optical tweezers, and adiabatic compression of surface plasmon polaritons. We show that the optical forces, caused by the plasmonic field, depend on the local response of the substrates and, in principle, allow probing both the real and the imaginary part of the local permittivity with a spatial resolution of few nanometers

Diagnostics of the tropical tropopause layer from in-situ observations and CCM data

A suite of diagnostics is applied to in-situ aircraft measurements and one Chemistry-Climate Model (CCM) data to characterize the vertical structure of the Tropical Tropopause Layer (TTL). The diagnostics are based on vertical tracer profiles and relative vertical tracer gradients, using tropopause-referenced coordinates, and tracer-tracer relationships in the tropical Upper Troposphere/Lower Stratosphere (UT/LS). Observations were obtained during four tropical campaigns performed from 1999 to 2006 with the research aircraft Geophysica and have been compared to the output of the ECHAM5/MESSy CCM. The model vertical resolution in the TTL (~500 m) allows for appropriate comparison with high-resolution aircraft observations and the diagnostics used highlight common TTL features between the model and the observational data. The analysis of the vertical profiles of water vapour, ozone, and nitrous oxide, in both the observations and the model, shows that concentration mixing ratios exhibit a strong gradient change across the tropical tropopause, due to the role of this latter as a transport barrier and that transition between the tropospheric and stratospheric regimes occurs within a finite layer. The use of relative vertical ozone and carbon monoxide gradients, in addition to the vertical profiles, helps to highlight the region where this transition occurs and allows to give an estimate of its thickness. The analysis of the CO-O3 and H2O-O3 scatter plots and of the Probability Distribution Function (PDF) of the H2O-O3 pair completes this picture as it allows to better distinguish tropospheric and stratospheric regimes that can be identified by their different chemical composition. The joint analysis and comparison of observed and modelled data allows to state that the model can represent the background TTL structure and its seasonal variability rather accurately. The model estimate of the thickness of the interface region between tropospheric and stratospheric regimes agrees well with average values inferred from observations. On the other hand, the measurements can be influenced by regional scale variability, local transport processes as well as deep convection, that can not be captured by the model

Information in a network of neuronal cells: Effect of cell density and short-term depression

Neurons are specialized, electrically excitable cells which use electrical to chemical signals to transmit and elaborate information. Understanding how the cooperation of a great many of neurons in a grid may modify and perhaps improve the information quality, in contrast to few neurons in isolation, is critical for the rational design of cell-materials interfaces for applications in regenerative medicine, tissue engineering, and personalized lab-on-a-chips. In the present paper, we couple an integrate-and-fire model with information theory variables to analyse the extent of information in a network of nerve cells. We provide an estimate of the information in the network in bits as a function of cell density and short-term depression time. In the model, neurons are connected through a Delaunay triangulation of not-intersecting edges; in doing so, the number of connecting synapses per neuron is approximately constant to reproduce the early time of network development in planar neural cell cultures. In simulations where the number of nodes is varied, we observe an optimal value of cell density for which information in the grid is maximized. In simulations in which the posttransmission latency time is varied, we observe that information increases as the latency time decreases and, for specific configurations of the grid, it is largely enhanced in a resonance effect

Diagnostics of the tropical tropopause layer from in-situ observations and CCM data

A suite of diagnostics is applied to in-situ aircraft measurements and one Chemistry-Climate Model (CCM) data to characterize the vertical structure of the Tropical Tropopause Layer (TTL). The diagnostics are based on the vertical tracers profiles, relative vertical tracers gradients, and tracer-tracer relationships in the tropical Upper Troposphere/Lower Stratosphere (UT/LS), using tropopause coordinates. Observations come from the four tropical campaigns performed from 1998 to 2006 with the research aircraft Geophysica and have been directly compared to the output of the ECHAM5/MESSy CCM. The model vertical resolution in the TTL allows for appropriate comparison with high-resolution aircraft observations and the diagnostics used highlight common TTL features between the model and the observational data. The analysis of the vertical profiles of water vapour, ozone, and nitrous oxide, in both the observations and the model, shows that concentration mixing ratios exhibit a strong gradient change across the tropical tropopause, due to the role of this latter as a transport barrier and that transition between the tropospheric and stratospheric regimes occurs within a finite layer. The use of relative vertical ozone gradients, in addition to the vertical profiles, helps to highlight the region where this transition occurs and allows to give an estimate of its thickness. The analysis of the CO-O3 and H2O-O3 scatter plots and of the Probability Distribution Function (PDF) of the H2O-O3 pair completes this picture as it allows to better distinguish tropospheric and stratospheric regimes that can be identified, first, by their differing chemical composition. The joint analysis and comparison of observed and modelled data allows us to evaluate the capability of the model in reproducing the observed vertical structure of the TTL and its variability, and also to assess whether observations from particular regions on a monthly timescale can be representative of the fine scale mean structure of the Tropical Tropopause Layer

MicroRNAs from saliva of anopheline mosquitoes mimic human endogenous miRNAs and may contribute to vector-host-pathogen interactions

During blood feeding haematophagous arthropods inject into their hosts a cocktail of salivary proteins whose main role is to counteract host haemostasis, inflammation and immunity. However, animal body fluids are known to also carry miRNAs. To get insights into saliva and salivary gland miRNA repertoires of the African malaria vector Anopheles coluzzii we used small RNA-Seq and identified 214 miRNAs, including tissue-enriched, sex-biased and putative novel anopheline miRNAs. Noteworthy, miRNAs were asymmetrically distributed between saliva and salivary glands, suggesting that selected miRNAs may be preferentially directed toward mosquito saliva. The evolutionary conservation of a subset of saliva miRNAs in Anopheles and Aedes mosquitoes, and in the tick Ixodes ricinus, supports the idea of a non-random occurrence pointing to their possible physiological role in blood feeding by arthropods. Strikingly, eleven of the most abundant An. coluzzi saliva miRNAs mimicked human miRNAs. Prediction analysis and search for experimentally validated targets indicated that miRNAs from An. coluzzii saliva may act on host mRNAs involved in immune and inflammatory responses. Overall, this study raises the intriguing hypothesis that miRNAs injected into vertebrates with vector saliva may contribute to host manipulation with possible implication for vector-host interaction and pathogen transmission