Evaluation of Virtual Measurements of Scanned Dental Impressions

Introduction: Digital models have been proposed as substitutes for plaster models. Dental arch impression scanning is a rapid and practical approach for digital model obtainment; however, few studies have accessed the accuracy of the method. This study verified the accuracy of virtual measurements obtained with the scanner Ortho Insight 3D, version 5.0 (Motionview Software, LLC, Chattanooga, Tennessee, USA). Materials And Methods: Total of 26 plaster models belonging to the Bahia Federal University Orthodontic postgraduation program were divided into three groups: G1 (plaster models); G2 (alginate impressions scan) and G3 (plaster models scan). Virtual measurements were compared to the manual by evaluating upper intercanine and intermolar distances and the antero posterior distance between upper left canine and upper left molar. Paired Student t and Lin agreement (5% of significance level) were employed for the statistical analysis. Results: Of the evaluated measures, the mean difference ranged from 0.48mm to 0.55mm when compared G2 and G1. The mean difference ranged from 0.6mm to 0.65mm between G3 and G1 groups. Comparing the groups G2 and G3 to G1, it was found statistically significant differences between all variables (p 0.99) for all parameters evaluated. Conclusion: Despite having existed significant differences between the virtual and physical approaches, substantial and almost perfect agreement between them allow us to suggest that there is accuracy of virtual images obtained from scanned impressions in alginate using the laser scanner Ortho Insight 3D

Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF

We present a high throughput shotgun mass spectrometry workflow using a bidimensional peptide fractionation procedure consisting of isoelectric focusing and RP-HPLC prior to mass spectrometric analysis, with the aim of optimizing peptide separation and protein identification. As part of the workflow we used the ‘Isotope-Coded Protein Labeling’ (ICPL) method for accurate relative quantitation of protein expression. Such workflow was successfully applied to a comparative proteome analysis of schizophrenia versus healthy control brain tissues and can be an alternative to proteome researches

Comparative gastrointestinal organ lengths among Amazonian primates (Primates: Platyrrhini)

ObjectivesThe morphological features of the gastrointestinal tract (GIT) in mammals reflect a species' food niche breadth and dietary adaptations. For many wild mammals, the relationship between the structure of the GIT and diet is still poorly understood, for example, the GIT for frugivorous primates is usually classified as unspecialized and homogeneous. Here, we compare the GIT structure of 13 primate species from the three families of extant platyrrhines (Atelidae, Pitheciidae, and Cebidae) in Amazonia, and discuss possible evolutionary adaptations to different diets and trophic niches.MethodsWe measured the length of the esophagus, stomach, small intestine, large intestine, cecum, colon, and rectum of the digestive tracts of 289 primate specimens. We determined the allometric relationships of the different tubular organs with the total length of the GIT as a proxy of specimen body size. Allometric parameters were used to establish the quotients of differentiation of every organ for each primate specimen.ResultsThere was a high differentiation in structure of the digestive organs among genera. Alouatta specimens clearly separated from the other genera based on dissimilarities in gastric, colonic, and rectal quotients, likely linked to the fermentation of plant contents. In contrast, all cebines (Sapajus, Cebus, and Saimiri) and Cacajao species had similar small intestine quotients, which is expected due to their high rates of animal matter consumed.ConclusionsWe show that diverse adaptations in digestive structure exist among frugivorous primates, which in turn reflect different dietary patterns within this group that may enable the geographic coexistence of different primate species