Alimentación a conciencia. Experiencia de práctica sociocomunitaria

En el 2014 se incorporaron las Prácticas Socio Comunitarias a la Facultad de Ingeniería de la Universidad Nacional de Mar del Plata. Mediante el “Aprendizaje-Servicio”, los y las estudiantes contextualizan los contenidos en los que se están formando al interactuar con la sociedad y su realidad. Se realizó un taller integrando docencia y extensión. Por un lado, participaron docentes e investigadoras/es y alumnos/as y por otro lado, la Cooperativa de Cuidadores Domiciliarios Mar del Plata e instituciones educativas. Se analizaron las deficiencias que poseen los actores de la comunidad respecto al “consumo seguro e informado” de los alimentos. A partir de ello es que las y los estudiantes, con la orientación de sus docentes tutores, recopilaron, analizaron y compararon con la legislación vigente información sobre alimentos light, apto celíaco, bajo en sodio, ultraprocesados y funcionales. Se generaron recursos audiovisuales logrando una comunidad de aprendizaje consciente de los criterios para seleccionar un alimento.In 2014, the Socio-Community Practices were incorporated into the Faculty of Engineering of the National University of Mar del Plata. Through “Service-Learning”, the students contextualize the contents in which they are being trained by interacting with society and its reality. A workshop was held integrating teaching and extension. On the one hand, teachers and researchers and students participated, and on the other hand, the Mar del Plata Home Caregiver Cooperative and educational institutions. The deficiencies that the community actors have regarding the “safe and informed consumption” of food were analyzed. Based on this, the students, with the guidance of their tutors, collected, analyzed and compared information on light foods, celiac-friendly, low-sodium, ultra-processed and functional foods with current legislation. Audiovisual resources were generated, achieving a learning community aware of the criteria to select a food.Fil: Agustinelli, Silvina Paola. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Investigación en Preservación y Calidad de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Benitez, Carmen. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Cátedra de Química General I; ArgentinaFil: Goñi, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Cátedra de Química General I; ArgentinaFil: Olaizola, Lourdes. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Pellegrini, María Celeste. Universidad Nacional de Mar del Plata. Facultad de Ingenieria. Instituto en Ciencia y Tecnología de Alimentos y Ambiente; . Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wasswrman, Cecilia. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Vázquez, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Schroeder, Carolina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Puglia, Iara Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Pages, Iara. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Lasaga, L. I.. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Garcia Machin, T.. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: De Pascuale, Sofia. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Coppa Legarreta, J. F.. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Cyras, Viviana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Manfredi, Lorena. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentin

Alimentación a conciencia. Experiencia de práctica sociocomunitaria

En el 2014 se incorporaron las Prácticas Socio Comunitarias a la Facultad de Ingeniería de la Universidad Nacional de Mar del Plata. Mediante el “Aprendizaje-Servicio”, los y las estudiantes contextualizan los contenidos en los que se están formando al interactuar con la sociedad y su realidad. Se realizó un taller integrando docencia y extensión. Por un lado, participaron docentes e investigadoras/es y alumnos/as y por otro lado, la Cooperativa de Cuidadores Domiciliarios Mar del Plata e instituciones educativas. Se analizaron las deficiencias que poseen los actores de la comunidad respecto al “consumo seguro e informado” de los alimentos. A partir de ello es que las y los estudiantes, con la orientación de sus docentes tutores, recopilaron, analizaron y compararon con la legislación vigente información sobre alimentos light, apto celíaco, bajo en sodio, ultraprocesados y funcionales. Se generaron recursos audiovisuales logrando una comunidad de aprendizaje consciente de los criterios para seleccionar un alimento.In 2014, the Socio-Community Practices were incorporated into the Faculty of Engineering of the National University of Mar del Plata. Through “Service-Learning”, the students contextualize the contents in which they are being trained by interacting with society and its reality. A workshop was held integrating teaching and extension. On the one hand, teachers and researchers and students participated, and on the other hand, the Mar del Plata Home Caregiver Cooperative and educational institutions. The deficiencies that the community actors have regarding the “safe and informed consumption” of food were analyzed. Based on this, the students, with the guidance of their tutors, collected, analyzed and compared information on light foods, celiac-friendly, low-sodium, ultra-processed and functional foods with current legislation. Audiovisual resources were generated, achieving a learning community aware of the criteria to select a food.Fil: Agustinelli, Silvina Paola. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Investigación en Preservación y Calidad de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Benitez, Carmen. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Cátedra de Química General I; ArgentinaFil: Goñi, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Cátedra de Química General I; ArgentinaFil: Olaizola, Lourdes. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Pellegrini, María Celeste. Universidad Nacional de Mar del Plata. Facultad de Ingenieria. Instituto en Ciencia y Tecnología de Alimentos y Ambiente; . Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wasswrman, Cecilia. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Vázquez, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Schroeder, Carolina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Puglia, Iara Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Pages, Iara. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Lasaga, L. I.. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Garcia Machin, T.. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: De Pascuale, Sofia. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Coppa Legarreta, J. F.. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; ArgentinaFil: Cyras, Viviana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Manfredi, Lorena. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentin

Complement lectin pathway activation is associated with COVID-19 disease severity, independent of MBL2 genotype subgroups

IntroductionWhile complement is a contributor to disease severity in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, all three complement pathways might be activated by the virus. Lectin pathway activation occurs through different pattern recognition molecules, including mannan binding lectin (MBL), a protein shown to interact with SARS-CoV-2 proteins. However, the exact role of lectin pathway activation and its key pattern recognition molecule MBL in COVID-19 is still not fully understood.MethodsWe therefore investigated activation of the lectin pathway in two independent cohorts of SARS-CoV-2 infected patients, while also analysing MBL protein levels and potential effects of the six major single nucleotide polymorphisms (SNPs) found in the MBL2 gene on COVID-19 severity and outcome.ResultsWe show that the lectin pathway is activated in acute COVID-19, indicated by the correlation between complement activation product levels of the MASP-1/C1-INH complex (p=0.0011) and C4d (p<0.0001) and COVID-19 severity. Despite this, genetic variations in MBL2 are not associated with susceptibility to SARS-CoV-2 infection or disease outcomes such as mortality and the development of Long COVID.ConclusionIn conclusion, activation of the MBL-LP only plays a minor role in COVID-19 pathogenesis, since no clinically meaningful, consistent associations with disease outcomes were noted

The nonlinear relaxation time and quasideterministic-theory approaches to characterize the decay of unestable states

We present the relationship between nonlinear-relaxation-time (NLRT) and quasideterministic approaches to characterize the decay of an unstable state. The universal character of the NLRT is established. The theoretical results are applied to study the dynamical relaxation of the Landau model in one and n variables and also a laser model

Genetic and expression analysis of CASP7 gene in a European Caucasian population with rheumatoid arthritis

INTRODUCTION A candidate gene approach, in a large case-control association study in the Dutch population, has shown that a 480 kb block on chromosome 4q27 encompassing KIAA1109/Tenr/IL2/IL21 genes is associated with rheumatoid arthritis. Compared with case-control association studies, family-based studies have the added advantage of controlling potential differences in population structure. Therefore, our aim was to test this association in populations of European origin by using a family-based approach. METHODS: A total of 1,302 West European white individuals from 434 trio families were genotyped for the rs4505848, rs11732095, rs6822844, rs4492018 and rs1398553 polymorphisms using the TaqMan Allelic discrimination assay (Applied Biosystems). The genetic association analyses for each SNP and haplotype were performed using the Transmission Disequilibrium Test and the genotype relative risk. RESULTS: We observed evidence for association of the heterozygous rs4505848-AG genotype with rheumatoid arthritis (P = 0.04); however, no significance was found after Bonferroni correction. In concordance with previous findings in the Dutch population, we observed a trend of undertransmission for the rs6822844-T allele and rs6822844-GT genotype to rheumatoid arthritis patients. We further investigated the five SNP haplotypes of the KIAA1109/Tenr/IL2/IL21 gene region. We observed, as described in the Dutch population, a nonsignificant undertransmission of the AATGG haplotype to rheumatoid arthritis patients. CONCLUSIONS: Using a family-based study, we have provided a trend for the association of the KIAA1109/Tenr/IL2/IL21 gene region with rheumatoid arthritis in populations of European descent. Nevertheless, we failed to replicate a significant association of this region in our rheumatoid arthritis family sample. Further investigation of this region, including detection and testing of all variants, is required to confirm rheumatoid arthritis association

Association between tumor necrosis factor receptor II and familial, but not sporadic, rheumatoid arthritis: Evidence for genetic heterogeneity

OBJECTIVE: Tumor necrosis factor alpha (TNFalpha) binds the receptors TNFRI and TNFRII. Results of genome scans have suggested that TNFR2 is a candidate rheumatoid arthritis (RA) locus. A case-control study in a UK Caucasian population has shown an association between a TNFR2 genotype (196R/R in exon 6) and familial, but not sporadic, RA. The present study was undertaken to test this association in the French Caucasian population. METHODS: To test for an association in sporadic RA, 100 families were genotyped for the 196M/R polymorphism and analyzed using the transmission disequilibrium test and haplotype relative risk. To test for an association in familial RA, RA index cases from 100 affected sibpair (ASP) families were genotyped for 196M/R. Linkage analysis was performed with 3 TNFR2 microsatellite markers. RESULTS: The TNFR2 196R/R genotype was not associated with sporadic RA (odds ratio [OR] 0.59, P = 0.72), but was associated with familial RA (OR 4.0, P = 0.026). The association was most marked in the context of TNFR2 "twin-like" RA sibs (affected sibs sharing both TNFR2 haplotypes) (OR 9.2, P = 0.0017). Linkage analysis results were consistent with the association; most of the TNFR2 linkage evidence was found in the subgroup of families with 196R/R ASP index cases. CONCLUSION: This study is the first to replicate evidence of the involvement of TNFR2 in RA genetic heterogeneity. Our data refine the initial hypothesis, to suggest that a TNFR2 recessive factor, in linkage disequilibrium with the 196R allele, plays a major role in a subset of families with multiple cases of RA

Relationship between SNPs and expression level for candidate genes in rheumatoid arthritis

Objectives: The study of polymorphisms of genes differentially expressed may lead to the identification of putative causal genetic variants in multifactorial diseases such as rheumatoid arthritis (RA). Based on preceding transcriptomic results, we genotyped 10 single nucleotide polymorphisms (SNPs) belonging to six genes (S100A8, RNASE2, PGLYRP1, RUNX3, IL2RB, and LY96) showing the highest fold change (> 1.9) when level of expression was compared between RA patients and controls. These SNPs were then analysed to evaluate their role in RA. Method: The relationship between gene expression and genotypes of SNPs was first investigated by Kruskal-Wallis and Mann-Whitney tests in RA patients and controls. The genetic association of these SNPs with RA were then analysed using family-based association tests in trio families. Results: We found that RNASE2 gene expression was related to rs2013109 genotypes in 14 RA patients (p = 0.030). The association study in a discovery sample of 200 French trio families revealed a significant association with RA for one SNP, PGLYRP1-rs2041992 (p = 0.019); this association was stronger in trios where RA patients carried the HLA-DRB1 shared epitope (SE) (p = 0.003). However, this association was not found in a replication sample of 240 European trio families (p = 0.6). Conclusions: Family-based association tests did not reveal an association between RA and any SNP of the candidate genes tested. However, RNASE2 gene expression was differentially expressed in RA patients considering a sequence polymorphism. This result led us to highlight the potential disease-specific regulation for this candidate gene in RA

The relationship between clinical signs and dry eye symptoms

Purpose To evaluate (i) the relationship between traditional and new clinical tests (lid-wiper epitheliopathy (LWE), lid-parallel conjunctival folds (LIPCOF)) and dry eye symptoms in non-contact lens wearers, and (ii) that a combination of these tests can improve predictive ability for the development of dry eye symptoms. Methods Tear meniscus height (TMH), non-invasive break-up time (NIBUT), ocular hyperaemia, LIPCOF, phenol red thread test (PRTT), corneal and conjunctival staining, and LWE grades were observed in a cohort of 47 healthy, non-lens wearers (male=17, female=30, median age=35 years, range=19–70). Symptoms were assessed using the Ocular Surface Disease Index (OSDI). Results LWE was significantly correlated to both temporal and nasal LIPCOF (0.537−0.248, P|0.31|; P<0.05). Significant discriminators of OSDI+/− were NIBUT (area under the receiver operative characteristic curve (AUC)=0.895), TMH (0.715), PRTT (0.781), LIPCOF (temporal/nasal/Sum 0.748/0.828/0.816), and LWE (0.749). Best predictive ability was achieved by combining NIBUT with nasal LIPCOF (AUC=0.944). Conclusions The individual tests NIBUT, TMH, PRTT, LIPCOF, and LWE were significantly, but moderately, related to OSDI scores. The strongest relationship appeared by combining NIBUT with nasal LIPCOF