Static aerodynamic characteristics of the S-IC booster/GAC H-33 orbiter launch vehicle configuration

This test at the trisonic tunnel was divided into two parts. The first part utilized the H-33 orbiter with drop tanks and cradle mounted on an S-IC booster with 75 square-foot fins oriented 45 deg off the horizontal and vertical reference planes. Runs were initially made to determine the optimum incidence angle to be used for the remainder of the tests. The angle so determined was 0 degrees. Additional testing was performed with drop tanks removed, orbiter vertical tail removed, drop tanks and orbiter wing removed, booster fins removed and booster fins located in the horizontal and vertical reference planes. The second part utilized the H-33 orbiter and cradle only, sting mounted on a strain gage balance. These data were used in conjunction with test number 504 (orbiter alone on balance) to determine cradle effects

The use of selected reaction monitoring in quantitative proteomics

Selected reaction monitoring (SRM) has a long history of use in the area of quantitative MS. In recent years, the approach has seen increased application to quantitative proteomics, facilitating multiplexed relative and absolute quantification studies in a variety of organisms. This article discusses SRM, after introducing the context of quantitative proteomics (specifically primarily absolute quantification) where it finds most application, and considers topics such as the theory and advantages of SRM, the selection of peptide surrogates for protein quantification, the design of optimal SRM co-ordinates and the handling of SRM data. A number of published studies are also discussed to demonstrate the impact that SRM has had on the field of quantitative proteomics. </jats:p

Bioassays- Procedures and Results

Bioassay procedures to describe, evaluate, and predict potential hazard of toxic materials to organisms, ecosystems, and health-related aspects of polluted waters continue to receive widespread attention. However, synthesizing these diverse theoretical, methodological, and procedural entities into an integrated multidisciplinary approach to evaluate environ mental hazards of toxic substances remains difficult. Symposia proceedings pertinent to the toxic substances management in ecosystems have appeared1 and provide an overview of major problems and emerging solutions, including chapters on new bioassay protocols development, and multiple exposure toxicity paradigms. Conclusions and recommendations resulting from a toxicity testing workshop2 discussed current state-of-the-art, identified needed improvements in methodology and research priorities, and called for coordinated multidisciplinary activities regarding physico-chemical, photodegradation, biodegradation, accumulation, and ecotoxicology test protocols. A comprehensive review of test methods for ecotoxicology was prepared by the National Research Council,3 in conjunction with a companion document of working papers prepared for committee use.4 The report was critical of single-species tests to predict ecosystematic effects, and presented detailed discussions of chemical toxicity assessment, factors influencing chemical fate in terrestrial and aquatic ecosystems, relevant properties and processes, appropriate test systems and end points, and a detailed assessment strategy employing integrated use of test systems. Multispecies tests to address eco logical toxicity have been critically reviewed and evaluated,5 including methods for measuring chemical effects on aquatic and terrestrial population interactions and ecosystem properties. A primary conclusion is the need for development and standardization of tests for effects of chemicals on ecological parameters that are indicative of interspecific interactions, community dynamics, and ecosystem function

Developing an On-Line Interactive Health Psychology Module.

On-line teaching material in health psychology was developed which ensured a range of students could access appropriate material for their course and level of study. This material has been developed around the concept of smaller 'content chunks' which can be combined into whole units of learning (topics), and ultimately, a module. On the basis of the underlying philosophy that the medium is part of the message, we considered interactivity to be a key element in engaging the student with the material. Consequently, the key aim of this development was to stimulate and engage students, promoting better involvement with the academic material, and hence better learning. It was hoped that this was achieved through the development of material including linked programmes and supporting material, small Java Scripts and basic email, forms and HTML additions. This material is outlined as are some of the interactive activities introduced, and the preliminary student and tutor experience described

Cardiac myocyte-specific knock-out of calcium-independent phospholipase A2γ (iPLA2γ) decreases oxidized fatty acids during ischemia/reperfusion and reduces infarct size

Calcium-independent phospholipase A(2)γ (iPLA(2)γ) is a mitochondrial enzyme that produces lipid second messengers that facilitate opening of the mitochondrial permeability transition pore (mPTP) and contribute to the production of oxidized fatty acids in myocardium. To specifically identify the roles of iPLA(2)γ in cardiac myocytes, we generated cardiac myocyte-specific iPLA(2)γ knock-out (CMiPLA(2)γKO) mice by removing the exon encoding the active site serine (Ser-477). Hearts of CMiPLA(2)γKO mice exhibited normal hemodynamic function, glycerophospholipid molecular species composition, and normal rates of mitochondrial respiration and ATP production. In contrast, CMiPLA(2)γKO mice demonstrated attenuated Ca(2+)-induced mPTP opening that could be rapidly restored by the addition of palmitate and substantially reduced production of oxidized polyunsaturated fatty acids (PUFAs). Furthermore, myocardial ischemia/reperfusion (I/R) in CMiPLA(2)γKO mice (30 min of ischemia followed by 30 min of reperfusion in vivo) dramatically decreased oxidized fatty acid production in the ischemic border zones. Moreover, CMiPLA(2)γKO mice subjected to 30 min of ischemia followed by 24 h of reperfusion in vivo developed substantially less cardiac necrosis in the area-at-risk in comparison with their WT littermates. Furthermore, we found that membrane depolarization in murine heart mitochondria was sensitized to Ca(2+) by the presence of oxidized PUFAs. Because mitochondrial membrane depolarization and calcium are known to activate iPLA(2)γ, these results are consistent with salvage of myocardium after I/R by iPLA(2)γ loss of function through decreasing mPTP opening, diminishing production of proinflammatory oxidized fatty acids, and attenuating the deleterious effects of abrupt increases in calcium ion on membrane potential during reperfusion

Global Bounds for the Lyapunov Exponent and the Integrated Density of States of Random Schr\"odinger Operators in One Dimension

In this article we prove an upper bound for the Lyapunov exponent $\gamma(E)$ and a two-sided bound for the integrated density of states $N(E)$ at an arbitrary energy $E>0$ of random Schr\"odinger operators in one dimension. These Schr\"odinger operators are given by potentials of identical shape centered at every lattice site but with non-overlapping supports and with randomly varying coupling constants. Both types of bounds only involve scattering data for the single-site potential. They show in particular that both $\gamma(E)$ and $N(E)-\sqrt{E}/\pi$ decay at infinity at least like $1/\sqrt{E}$. As an example we consider the random Kronig-Penney model.Comment: 9 page