Possible coupling between climatically induced lake level change, volcanic eruptions and seismotectonic activation in the Rukwa-Rungwe-Nyasa rift, SW Tanzania

The Rukwa rift basin is presently a closed hydrogeological depression containing a shallow lake (max 20 me deep) with its surface at an altitude around 810 m above sea level. Lacustrine terraces and paleo-shorelines are known up to 980 m above sea level, an altitude at which it reach the overflow sill towards Lake Tanganyika. Both Lakes Tanganyika and Nyasa (Malawi) are presently overflowing, but as their lake level fluctuates, they have been disconnected from their outlet in the recent past. High resolution seismic profiling in both Lakes Rukwa and Malawi has show the presence of active fault systems underneath the lake floor. Some of these fault systems appear to have had a cyclic activity, with alternating periods of high tectonic activity/sedimentation and periods of tectonic quiescence. The accommodation zone between Lake Rukwa and Nyasa is occupied by the Rungwe volcanic Province, with the Ngozi, Rungwe and Kiejo volcanoes presenting signs of recent volcanic activity. The Rungwe Province is cross-cut by several directions of faults, which clearly control the location of the volcanic vents.In our work, we reviewed the available data on recent (Late Pleistocene – Holocene) volcanic eruptions, in the Rungwe area itself, in the drill cores from the surrounding lakes and from aerial observations up to 300 km away from the Rungwe Province. We performed morphotectonic and paleoseismic investigations of the Kanda fault, a major normal fault between lakes Rukwa and Tanganyika. We investigated lacustrine deposits of the Rukwa basin corresponding to the two last cycles of high lake level. The chronological framework was established using 30 new radiocarbon dating and the most prominent volcanic tephra layers were used as a reference in the correlations. The results are still preliminary, but a good correlation already appear between climatically induced lake level change (in Lake Rukwa), seismo-tectonic activation of the regional fault network (underneath Lake Rukwa and the Kanda fault between Lakes Rukwa and Tanganyika) and the timing of the recent strong volcanic eruptions in the Rungwe Volcanic Province since the last 40.000 years. This relation is explained taking into account that Lake Rukwa is very sensitive to climate change as it occupies a flat depression and its overflow outlet is 180 m above its present-day level. Its lake level rises rapidly when the climate becomes more humid as it was the case during the Last Glacial Maximum and during the Younger Dryas event. Increase in lake level means increasing of the load in the basin and perturbation of the ambient tectonic stresses. In most of the Rukwa rift, the tectonic stress is of extensional (normal faulting) regime, with the maximum principal stress axis (sigma 1) subvertical. In these conditions, increasing the vertical load will increase the shear stress on the existing normal faults, triggering (seismogenic) normal faulting deformation. As the architecture of the active volcanoes in the Rungwe Province is tectonically controlled, activation of the faults, together with a greater pressure of water in the tectonic discontinuities are likely to trigger large volcanic eruptions, strongly explosive

Earthquake geology of the Kanda fault system (Tanganyika-Rukwa rift, SW highlands of Tanzania)

The 160 km-long Kanda normal fault system is running longitudinally across the Ufipa tilted horst between the Tanganyika and Rukwa rift basins in the western branch of the East African Rift System. It is likely to have been affected the Ms 7.4 earthquake in 1910 whose instrumental epicentre was located approximately near Sumbawanga town, along the northern portion of the fault. Remote sensing, morphotectonic, and palaeoseismological investigations together with structural geology and electric resistivity profiling allow to characterise the main seismological parameters of this major active fault.In order to reconstruct the along-trend displacement profile and fault segmentation, topographic profiles were made across the fault scarp using data from differential GPS, Hand GPS and the 90 m resolution STRM DEM. Comparison of topographic profiles from the same site using the different topographic data provides some insight on the methodology and validity of topographic profiles extraction. Due to the large dimensions of the fault scarp (10-30 m high) the strong magnitude of the potential earthquakes (Ms. 7.5) and the large associated co-seismic slip (2-4 m), classical paleoseismic trenching is difficult to apply. Instead, we used existing exposures such as natural trenches, road cuts and outcrops, supplemented by a dense topographic survey (5000 points on differential GPS over less than 1 km2) to produce a 2 m resolution digital elevation model, performed detailed geological mapping, airphoto interpretation, electric resistivity profiling, small drillings, and C14 dating. Efforts were concentrated on a promising site where river sediments and peat deposits are interbedded with volcanic tephra layers and largely exposed on the footwall. Results show that the Kanda fault had different cycles of activity, separated by tectonically quite periods. The last period of activity probably started by 11 cal Ka BP, soon after the deposition of a marked tephra layer, but sedimentation on the footwall continued up to 5 Ka cal BP. Since then, the minimum offset is estimated at 17.9 m, which gives a minimum slip rate of 3.58 mm/year, of an equivalent of one earthquakes generating 3.58 m slip per 1000 years (Ms in the range of 7.0 - 7.5)

Community knowledge, attitude and practice towards Sexually Transmitted Diseases and HIV infection in Biharamulo and Muleba districts in Kagera Region, Tanzania

Abstract: The study was conducted to determine knowledge, attitude and practice towards Sexually Transmitted Diseases and HIV infections among communities in Biharamulo and Muleba districts, Kagera Tanzania. A total of 915 study participants were recruited and most of them (96.3%) knew that there are diseases which could be transmitted through sexual contact. Seventy one percent of participants thought STDs could be acquired through sharing a towel while fifty percent thought HIV could be transmitted through insect bites. Eighty five percent of school pupils who participated in the study reported to have been taught about AIDS and less than 30% on sex and pregnancy. Sixty three percent of study participants were of the opinion that a girl or woman should not refuse to have sex after being given a gift, and having sex with an elder partner was thought to be acceptable by almost fifty percent of participants. Over 50% percent of interviewees thought a girl or woman should not refuse to have sex with their friends. Although 99% of interviewees reported to have ever heard about condoms, only 28% reported to have ever used them irrespective of been affordable. Most schoolboys and about 50% of schoolgirls reported to have experienced sex by the time of the study. Thirty eight percent of girls reported to have first sex at the age of 14 years. Nine percent of the participants who reported to have experienced sex were forced to do so. Knowledge regarding STDs and HIV/AIDS was high among participants, but a sizeable proportion report misconception on transmission of STDs/HIV such as through sharing a towel and insect bites. Therefore it is recommended that S&RH intervention programme should address these misconceptions in order to match knowledge and practice, and achieve the intended objectives

Hunger, waiting time and transport costs: Time to confront challenges to ART adherence in Africa

International audienceAdherence levels in Africa have been found to be better than those in the US. However around one out of four ART users fail to achieve optimal adherence, risking drug resistance and negative treatment outcomes. A high demand for 2nd line treatments (currently ten times more expensive than 1st line ART) undermines the sustainability of African ART programs. There is an urgent need to identify context-specific constraints to adherence and implement interventions to address them. We used rapid appraisals (involving mainly qualitative methods) to find out why and when people do not adhere to ART in Uganda, Tanzania and Botswana. Multidisciplinary teams of researchers and local health professionals conducted the studies, involving a total of 54 semi-structured interviews with health workers, 73 semi-structured interviews with ART users and other key informants, 34 focus group discussions, and 218 exit interviews with ART users. All the facilities studied in Botswana, Tanzania and Uganda provide ARVs free of charge, but ART users report other related costs (e.g. transport expenditures, registration and user fees at the private health facilities, and lost wages due to long waiting times) as main obstacles to optimal adherence. Side effects and hunger in the initial treatment phase are an added concern. We further found that ART users find it hard to take their drugs when they are among people to whom they have not disclosed their HIV status, such as co-workers and friends. The research teams recommend that (i) health care workers inform patients better about adverse effects; (ii) ART programmes provide transport and food support to patients who are too poor to pay; (iii) recurrent costs to users be reduced by providing three-months, rather than the one-month refills once optimal adherence levels have been achieved; and (iv) pharmacists play an important role in this follow-up care

Risk Factors for Buruli Ulcer: A Case Control Study in Cameroon

Buruli ulcer (BU) is a neglected tropical infectious disease caused by Mycobacterium ulcerans. While BU is associated with areas where the water is slow-flowing or stagnant, the exact mechanism of transmission of the bacillus is unknown, impairing efficient control programs. Two hypotheses are proposed in the literature: previous trauma at the lesion site, and transmission through aquatic insect bites. Using results from a face-to-face questionnaire, our study compared characteristics from Cameroonian patients with Buruli ulcer to people without Buruli ulcer. This latter group of people was chosen within the community or within the family of case patients. The statistical analysis confirmed some well-known factors associated with the presence of BU, such as wearing short lower-body clothing while farming, but it showed that the use of bed nets and the treatment of wounds with leaves is less frequent in case patients. These newly identified factors may provide new insight into the mode of transmission of M. ulcerans. The implication of domestic or peridomestic insects, suggested by the influence of the use of bed nets, should be confirmed in specific studies

Identification of field caught Anopheles gambiae s.s. and Anopheles arabiensis by TaqMan single nucleotide polymorphism genotyping

BACKGROUND: Identification of Anopheles gambiae s.s. and Anopheles arabiensis from field-collected Anopheles gambiae s.l. is often necessary in basic and applied research, and in operational control programmes. The currently accepted method involves use of standard polymerase chain reaction amplification of ribosomal DNA (rDNA) from the 3' 28S to 5' intergenic spacer region of the genome, and visual confirmation of amplicons of predicted size on agarose gels, after electrophoresis. This report describes development and evaluation of an automated, quantitative PCR method based upon TaqMan™ single nucleotide polymorphism (SNP) genotyping. METHODS: Standard PCR, and TaqMan SNP genotyping with newly designed primers and fluorophore-labeled probes hybridizing to sequences of complementary rDNA specific for either An. gambiae s.s. or An. arabiensis, were conducted in three experiments involving field-collected An. gambiae s.l. from western Kenya, and defined laboratory strains. DNA extraction was from a single leg, sonicated for five minutes in buffer in wells of 96-well PCR plates. RESULTS: TaqMan SNP genotyping showed a reaction success rate, sensitivity, and species specificity comparable to that of standard PCR. In an extensive field study, only 29 of 3,041 (0.95%) were determined to be hybrids by TaqMan (i.e., having rDNA sequences from both species), however, all but one were An. arabiensis by standard PCR, suggesting an acceptably low (ca. 1%) error rate for TaqMan genotyping in mistakenly identifying species hybrids. CONCLUSION: TaqMan SNP genotyping proved to be a sensitive and rapid method for identification of An. gambiae s.l. and An. arabiensis, with a high success rate, specific results, and congruence with the standard PCR method

Bringing evidence to bear for negotiating tradeoffs in sustainable agricultural intensification using a structured stakeholder engagement process

Sustainable agricultural intensification (SAI) has the potential to increase food security without detrimental effects on ecosystem services. However, adoption of SAI practices across sub-Saharan Africa has not reached transformational numbers to date. It is often hampered by lack of context-specific practices, sub-optimal understanding of tradeoffs and synergies among stakeholders, and lack of approaches that bring diverse evidence sources together with stakeholders to collectively tackle complex problems. In this study, we asked three interconnected questions: (i) What is the accessibility and use of evidence for SAI decision making; (ii) What tools could enhance access and interaction with evidence for tradeoff analysis; and (iii) Which stakeholders must be included? This study employed a range of research and engagement methods including surveys, stakeholder analysis, participatory trade-off assessments and co-design of decision dashboards to better support evidence-based decision making in Zambia, Tanzania and Ethiopia. At the inception, SAI evidence was accessible and used by less than half of the decision makers across the three countries and online dashboards hold promise to enhance access. Many of the stakeholders working on SAI were not collaborating and tradeoff analysis was an under-utilized tool. Structured engagement across multiple stakeholder groups with evidence is critical

Ethnobotanical study of some of mosquito repellent plants in north-eastern Tanzania

The use of plant repellents against nuisance biting insects is common and its potential for malaria vector control requires evaluation in areas with different level of malaria endemicity. The essential oils of Ocimum suave and Ocimum kilimandscharicum were evaluated against malaria vectors in north-eastern Tanzania. An ethnobotanical study was conducted at Moshi in Kilimanjaro region north-eastern Tanzania, through interviews, to investigate the range of species of plants used as insect repellents. Also, bioassays were used to evaluate the protective potential of selected plants extracts against mosquitoes. The plant species mostly used as repellent at night are: fresh or smoke of the leaves of O. suave and O. kilimandscharicum (Lamiaceae), Azadirachta indica (Meliaceae), Eucalyptus globules (Myrtaceae) and Lantana camara (Verbenaceae). The most popular repellents were O. kilimandscharicum (OK) and O. suave (OS) used by 67% out of 120 households interviewed. Bioassay of essential oils of the two Ocimum plants was compared with citronella and DEET to study the repellence and feeding inhibition of untreated and treated arms of volunteers. Using filter papers impregnated with Ocimum extracts, knockdown effects and mortality was investigated on malaria mosquito Anopheles arabiensis and Anopheles gambiae, including a nuisance mosquito, Culex quinquefasciatus. High biting protection (83% to 91%) and feeding inhibition (71.2% to 92.5%) was observed against three species of mosquitoes. Likewise the extracts of Ocimum plants induced KD90 of longer time in mosquitoes than citronella, a standard botanical repellent. Mortality induced by standard dosage of 30 mg/m2 on filter papers, scored after 24 hours was 47.3% for OK and 57% for OS, compared with 67.7% for citronella. The use of whole plants and their products as insect repellents is common among village communities of north-eastern Tanzania and the results indicate that the use of O. suave and O. kilimandscharicum as a repellent would be beneficial in reducing vector biting. The widespread use of this approach has a potential to complement other control measures