Effect of "spraying" by fighting honey bee queens (Apis mellifera L.) on the temporal structure of fights

To reproduce, social insect colonies rear sexual progeny, and young queens start a new colony either without (independently) or with the help of workers (dependently). When colony reproduction is dependent and young queens are produced in excess, conflicts are predicted to occur. Honey bee colonies reproduce dependently by swarming. The mother queen leaves with a Óprime swarmÓ before daughter queens reach adulthood. More queens are produced than can obtain sufficient worker force, and emerging queens often fight to death. Surviving queen(s) inherit the established nest or a portion of workers which then depart in an ÓafterswarmÓ. Honey bee queens show numerous adaptations for fighting and conflict with other queens, such as early venom production and fast development. During fights one queen often releases rectal fluid. The function of this ÓsprayingÓ behaviour is unclear. Previous studies have reported that it can both attract and repel workers, and observations that it also interrupts fights. Possible functions of spraying are to affect worker intervention in fights, act as a chemical weapon, or lower worker attention towards contaminated queens. We staged fights between 24 queen pairs to investigate the temporal pattern of behaviour in spraying and non-spraying fights. Spraying occurred in 67% of the fights. In the majority of spraying fights (87%) it occurred upon physical contact. Spraying fights were characterized by significantly lower proportion of escalated aggression and a significantly shorter first escalated bout. This provides quantitative evidence that spraying interrupts fights and suggests that its function is to directly provide a temporary respite to queens

Characterization of queen specific components of the fluid released by fighting honey bee queens

Swarming honey bee (Apis mellifera L.) colonies rear supernumerary young queens that compete for the limited resources (workers) necessary for founding a new colony. Young queens often fight to death. Queens show several adaptations to fight and conflict, such as short developmental time and early onset of venom production. During fights, queens often release rectal fluid with a strong smell of wine grapes, after which they temporarily stop fighting. This potentially reduces individual overall risk of deadly injury. The fluid and one of its components, ortho-aminoacetophenone, were previously found to have a pheromonal effect on workers, but the evidence is equivocal. Recently, it has been suggested that the effects of this substance may be context- or concentration-specific. We performed semi-quantitative gas-chromatography mass spectrometry (GC/MS) analysis of the fluid (1) released by queens during their first fight, (2) released during a subsequent fight, and (3) obtained by dissecting the hindgut of queens and (4) of workers. Following preliminary results by Page et al. 1988 (Experientia 44: 270-271), we scored presence / absence of eight substances. Five substances (ortho-aminoacetophenone, decanoic acid, dodecanoic acid, octyl decanoate, and decyl decanoate) were characteristic of queens only. ortho-Aminoacetophenone was detected in all queen and in none of the worker samples, in agreement with previous findings that worker faeces do not have any pheromonal effect. The fluid released by queens on their second fight also contained ortho-aminoacetophenone, but in smaller quantities. These data substantiate previous bioassay results, and provide estimates of ortho-aminoacetophenone concentration as required to design experiments addressing the function and adaptive significance of this behaviour

Working-class royalty: bees beat the caste system

The struggle among social classes or castes is well known in humans. Here, we show that caste inequality similarly affects societies of ants, bees and wasps, where castes are morphologically distinct and workers have greatly reduced reproductive potential compared with queens. In social insects, an individual normally has no control over its own fate, whether queen or worker, as this is socially determined during rearing. Here, for the first time, we quantify a strategy for overcoming social control. In the stingless bee Schwarziana quadripunctata, some individuals reared in worker cells avoid a worker fate by developing into fully functional dwarf queens

Behavior Analysis of Ants from Video Sequences

The movement of small animals in well-defined environments is increasingly studied in many areas; including ecotoxicology, learning, and behavioural ecology. Here we de- scribe an algorithm designed to analyze individual foraging ants from a colony of Lasius niger. The inputs to the algorithm were images from a video sequence. The algorithm performed a series of pre-processing steps to identify the ants from the pixels, measurements were extracted and individual ants were tracked in time. The location of the ants in position and time were recorded as heat maps denoting the favorite locations of the ants. The ants were videoed in a foraging experiment on a T-maze a single trail bifurcation

Gut microbiota composition is associated with environmental landscape in honey bees.

There is growing recognition that the gut microbial community regulates a wide variety of important functions in its animal hosts, including host health. However, the complex interactions between gut microbes and environment are still unclear. Honey bees are ecologically and economically important pollinators that host a core gut microbial community that is thought to be constant across populations. Here, we examined whether the composition of the gut microbial community of honey bees is affected by the environmental landscape the bees are exposed to. We placed honey bee colonies reared under identical conditions in two main landscape types for 6 weeks: either oilseed rape farmland or agricultural farmland distant to fields of flowering oilseed rape. The gut bacterial communities of adult bees from the colonies were then characterized and compared based on amplicon sequencing of the 16S rRNA gene. While previous studies have delineated a characteristic core set of bacteria inhabiting the honey bee gut, our results suggest that the broad environment that bees are exposed to has some influence on the relative abundance of some members of that microbial community. This includes known dominant taxa thought to have functions in nutrition and health. Our results provide evidence for an influence of landscape exposure on honey bee microbial community and highlight the potential effect of exposure to different environmental parameters, such as forage type and neonicotinoid pesticides, on key honey bee gut bacteria. This work emphasizes the complexity of the relationship between the host, its gut bacteria, and the environment and identifies target microbial taxa for functional analyses

Automatic Analysis of Bees’ Waggle Dance

This work describe an algorithm for the automatic analysis of the waggle dance of honeybees. The algorithm analyses a video of a beehive with 13,624 frames, acquired at 25 frames/second. The algorithm employs the following traditional image processing steps: conversion to grayscale, low pass filtering, background subtraction, thresholding, tracking and clustering to detect run of bees that perform waggle dances. The algorithm detected 44,530 waggle events, i.e. one bee waggling in one time frame, which were then clustered into 511 waggle runs. Most of these were concentrated in one section of the hive. The accuracy of the tracking was 90% and a series of metrics like intra-dance variation in angle and duration were found to be consistent with literature. Whilst this algorithm was tested on a single video, the ideas and steps, which are simple as compared with Machine and Deep Learning techniques, should be attractive for researchers in this field who are not specialists in more complex techniques

Bourgeois behavior and freeloading in the colonial orb web spider Parawixia bistriata (Araneae, Araneidae).

Spiders of the tropical American colonial orb weaver Parawixia bistriata form a communal bivouac in daytime. At sunset, they leave the bivouac and construct individual, defended webs within a large, communally built scaffolding of permanent, thick silk lines between trees and bushes. Once spiders started building a web, they repelled other spiders walking on nearby scaffolding with a bounce behavior. In nearly all cases (93%), this resulted in the intruder leaving without a fight, akin to the bourgeois strategy, in which residents win and intruders retreat without escalated contests. However, a few spiders (6.5%) did not build a web due to lack of available space.Webless spiders were less likely to leave when bounced (only 42% left) and instead attempted to freeload, awaiting the capture of prey items in nearby webs. Our simple model shows that webless spiders should change their strategy from bourgeois to freeloading satellite as potential web sites become increasingly occupied

Correlated expression of phenotypic and extended phenotypic traits across stingless bee species: worker eye morphology, foraging behaviour, and nest entrance architecture.

Abstract: Stingless bees are the most species-rich group of eusocial bees and show great diversity in behaviour, ecology, nest architecture, colony size, and worker morphology. How this variation relates to varying selection pressures and constraints is not well understood. Variation can be caused by selection acting on behavioural or morphological traits, both alone and in correlation across traits. Here we tested whether behavioural and morphological traits important for foraging and defence are linked to nest-entrance architecture, an extended phenotype relevant to both foraging and nest defence. Using 23 species we investigated whether eye size, nest entrance size, landing behaviour and foraging method show cross-species correlations. A phylogenetically-controlled comparative analysis revealed that species with relatively smaller eyes build relatively larger entrances, which in turn are associated with faster landing approaches and fewer landing errors by foragers, both of which could reduce predation risk. Concerning foraging, mass-recruiting species have c. 10-times larger entrance holes than species with a solitary foraging strategy. Larger entrances could help species with mass recruitment to rapidly increase forager traffic or mount a strong defensive response when under attack. Our results show that studying correlations among different traits helps understand phenotypic diversity in species rich groups

Anarchy in the UK: Detailed genetic analysis of worker reproduction in a naturally occurring British anarchistic honeybee, Apis mellifera, colony using DNA microsatellites

Anarchistic behaviour is a very rare phenotype of honeybee colonies. In an anarchistic colony, many workers’ sons are reared in the presence of the queen. Anarchy has previously been described in only two Australian colonies. Here we report on a first detailed genetic analysis of a British anarchistic colony. Male pupae were present in great abundance above the queen excluder, which was clearly indicative of extensive worker reproduction and is the hallmark of anarchy. Seventeen microsatellite loci were used to analyse these male pupae, allowing us to address whether all the males were indeed workers’ sons, and how many worker patrilines and individual workers produced them. In the sample, 95 of 96 of the males were definitely workers’ sons. Given that ≈ 1% of workers’ sons were genetically indistinguishable from queen’s sons, this suggests that workers do not move any queen-laid eggs between the part of the colony where the queen is present to the area above the queen excluder which the queen cannot enter. The colony had 16 patrilines, with an effective number of patrilines of 9.85. The 75 males that could be assigned with certainty to a patriline came from 7 patrilines, with an effective number of 4.21. They were the offspring of at least 19 workers. This is in contrast to the two previously studied Australian naturally occurring anarchist colonies, in which most of the workers’ sons were offspring of one patriline. The high number of patrilines producing males leads to a low mean relatedness between laying workers and males of the colony. We discuss the importance of studying such colonies in the understanding of worker policing and its evolution