Transgressing boundaries : gender, identity, culture, and 'other' in postcolonial women's narratives in Africa

EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Histone marks regulate the epithelial-to-mesenchymal transition via alternative splicing

International audienceHistone modifications impact final splicing decisions. However, there is little evidence of the driving role of these marks in inducing cell-specific splicing changes. Using CRISPR epigenome editing tools, we show in an epithelial-to-mesenchymal cell reprogramming system (epithelial-to-mesenchymal transition [EMT]) that a single change in H3K27ac or H3K27me3 levels right at the alternatively spliced exon is necessary and sufficient to induce a splicing change capable of recapitulating important aspects of EMT, such as cell motility and invasiveness. This histone-mark-dependent splicing effect is highly dynamic and mediated by direct recruitment of the splicing regulator PTB to its RNA binding sites. These results support a role for H3K27 marks in inducing a change in the cell's phenotype via regulation of alternative splicing. We propose the dynamic nature of chromatin as a rapid and reversible mechanism to coordinate the splicing response to cell-extrinsic cues, such as induction of EMT

Palaeoecological evaluation of the recent acidification of Lochnagar, Scotland

Lochnagar, a high altitude, relatively deep, come lake, lies on the Royal Deeside ESUHC of Balmoral, in an area which experiences moderate levels of acid deposition, The loch catchment comprises granite bedrock and is dominated by bare rock but overlain in places with blanket peals, Lochnagar may thus be considered potentially susceptible to acidification, The contemporary pH of the loch water is c. 5,0