Vitellogenin receptors during vitellogenesis in the rainbow trout Oncorhynchus mykiss

Rainbow trout vitellogenin receptors have been characterized by ligand blotting and Scatchard analysis. Their evolution has been studied over a reproductive cycle in a broodstock of 2-year-old females. The receptors were prepared from ovarian membrane homogenates and were solubilized using n-octyl-beta-D-glucopyranoside. The visualization of the receptor by ligand blotting using 125iodine-vitellogenin after sodium dodecyl sulfate electrophoresis revealed the existence of one major binding component corresponding to a protein of 113 kDa. The Scatchard transformation of the binding data revealed a single class of binding sites. The variations of the binding characteristics (Kd and maximum binding) were investigated during vitellogenesis. This study revealed that the Kd was not affected by oocyte growth during vitellogenesis, but was highly decreased in ovulated eggs. The receptor number increased during the same period from 35 to 860 fM per oocyte, while the receptor number per mm2 of oocyte membrane surface was doubled during the same period. (Résumé d'auteur

Light controls motility and phase separation of photosynthetic microbes

Large ensembles of interacting, out-of-equilibrium agents are a paradigm of active matter. Their constituents' intrinsic activity may entail the spontaneous separation into localized phases of high and low densities. Motile microbes, equipped with ATP-fueled engines, are prime examples of such phase-separating active matter, which is fundamental in myriad biological processes. The fact that spontaneous spatial aggregation is not widely recognized as a general feature of microbial communities challenges the generalisation of phase separation beyond artificial active systems. Here, we report on the phase separation of populations of Chlamydomonas reinhardtii that can be controlled by light in a fully reversible manner. We trace this phenomenon back to the light- and density-dependent motility, thus bridging the gap from light perception on the single-cell level to collective spatial self-organization into regions of high and low density. Its spectral sensitivity suggests that microbial motility and phase separation are regulated by the activity of the photosynthetic machinery. Characteristic fingerprints of the stability and dynamics of this active system paint a picture that cannot be reconciled with the current physical understanding of phase separation in artificial active matter, whereby collective behavior can emerge from inherent motility modulation in response to changing stimuli. Our results therefore point towards the existence of a broader class of self-organization phenomena in living systems

Speech Communication

Contains reports on five research projects.National Institutes of Health (Grant 5 RO1 NS04332-12)National Institutes of Health (Grant HD05168-04)U.S. Navy Office of Naval Research (Contract N00014-67-A-0204-0069)Joint Services Electronics Program (Contract DAAB07-74-C-0630)National Science Foundation (Grant SOC74-22167

A measurement of cosmic ray deuterium from 0.5–2.9 GeV/nucleon

The rare isotopes ^(2)H and ^(3)He in cosmic rays are believed to originate mainly from the interaction of high energy protons and helium with the galactic interstellar medium. The unique propagation history of these rare isotopes provides important constraints on galactic cosmic ray source spectra and on models for their propagation within the Galaxy. Hydrogen and helium isotopes were measured with the balloon-borne experiment, IMAX, which flew from Lynn Lake, Manitoba in 1992. The energy spectrum of deuterium between 0.5 and 3.2 GeV/nucleon measured by the IMAX experiment as well as previously published results of ^(3)He from the same instrument will be compared with predictions of cosmic ray galactic propagation models. The observed composition of the light isotopes is found to be generally consistent with the predictions of the standard Leaky Box Model derived to fit observations of heavier nucle

The Cosmic-Ray Proton and Helium Spectra measured with the CAPRICE98 balloon experiment

A new measurement of the primary cosmic-ray proton and helium fluxes from 3 to 350 GeV was carried out by the balloon-borne CAPRICE experiment in 1998. This experimental setup combines different detector techniques and has excellent particle discrimination capabilities allowing clear particle identification. Our experiment has the capability to determine accurately detector selection efficiencies and systematic errors associated with them. Furthermore, it can check for the first time the energy determined by the magnet spectrometer by using the Cherenkov angle measured by the RICH detector well above 20 GeV/n. The analysis of the primary proton and helium components is described here and the results are compared with other recent measurements using other magnet spectrometers. The observed energy spectra at the top of the atmosphere can be represented by (1.27+-0.09)x10^4 E^(-2.75+-0.02) particles (m^2 GeV sr s)^-1, where E is the kinetic energy, for protons between 20 and 350 GeV and (4.8+-0.8)x10^2 E^(-2.67+-0.06) particles (m^2 GeV nucleon^-1 sr s)^-1, where E is the kinetic energy per nucleon, for helium nuclei between 15 and 150 GeV nucleon^-1.Comment: To be published on Astroparticle Physics (44 pages, 13 figures, 5 tables

Measurement of 0.25-3.2 GeV antiprotons in the cosmic radiation

The balloon-borne Isotope Matter-Antimatter Experiment (IMAX) was flown from Lynn Lake, Manitoba, Canada on 16–17 July 1992. Using velocity and magnetic rigidity to determine mass, we have directly measured the abundances of cosmic ray antiprotons and protons in the energy range from 0.25 to 3.2 GeV. Both the absolute flux of antiprotons and the antiproton/proton ratio are consistent with recent theoretical work in which antiprotons are produced as secondary products of cosmic ray interactions with the interstellar medium. This consistency implies a lower limit to the antiproton lifetime of ∼10 to the 7th yr