471 research outputs found
Uncovering a Solvent-Controlled Preferential Growth of Buckminsterfullerene (C60) Nanowires
The fullerene (C60) nanowires, which possess a highly unusual morphology
featured by a prism-like central core and three nanobelt-like wings joined
along the growth direction to give an overall Y-shaped cross section, have been
studied. The experimental observation coupled with theoretical calculation have
enabled us to elucidate both the role of the fullerene and of the solvent in
the crystallization process, thus opening up an opportunity for the in-depth
understanding of the crystal growth mechanism. More generally, the method
developed in this work could be extended into understanding the growth of other
inorganic nanowires that have both host and guest molecules involved in their
crystal lattices.Comment: 25 pages, 9 figure
On calculating the probability of a set of orthologous sequences
Probabilistic DNA sequence models have been intensively applied to genome research. Within the evolutionary biology framework, this article investigates the feasibility for rigorously estimating the probability of a set of orthologous DNA sequences which evolve from a common progenitor. We propose Monte Carlo integration algorithms to sample the unknown ancestral and/or root sequences a posteriori conditional on a reference sequence and apply pairwise Needleman–Wunsch alignment between the sampled and nonreference species sequences to estimate the probability. We test our algorithms on both simulated and real sequences and compare calculated probabilities from Monte Carlo integration to those induced by single multiple alignment
The Search for Low-mass Companions of B Stars in the Carina Nebula Cluster Trumpler 16
We have developed lists of likely B3--A0 stars (called "late B" stars) in the
young cluster Trumpler 16. The following criteria were used: location within 3'
of Eta Car, an appropriate V and B-V combination, and proper motion (where
available). Color and magnitude cuts have been made assuming an E(B-V) =0.55
mag +/- 0.1, which is a good approximation close to the center of Trumpler 16.
These lists have been cross-correlated with X-ray sources found in the Chandra
Carina Complex Project (CCCP). Previous studies have shown that only very
rarely (if at all) do late main sequence B stars produce X-rays. We present
evidence that the X-ray detected sources are binaries with low-mass companions,
since stars less massive than 1.4 Msun are strong X-ray sources at the age of
the cluster. Both the median X-ray energies and X-ray luminosities of these
sources are in good agreement with values for typical low-mass coronal X-ray
sources. We find that 39% of the late B stars based on a list with proper
motions have low-mass companions. Similarly, 32% of a sample without proper
motions have low-mass companions. We discuss the X-ray detection completeness.
These results on low-mass companions of intermediate mass stars are
complementary to spectroscopic and interferometric results, and probe new
parameter space of low mass companions at all separations. They do not support
a steeply rising distribution of mass ratios to low masses for
intermediate-mass (5 Msun) primaries, such as would be found by random pairing
from the Initial Mass Function.Comment: Accepted for the ApJS Special Issue on the Chandra Carina Complex
Project (CCCP), scheduled for publication in May 2011. All 16 CCCP Special
Issue papers are available at
http://cochise.astro.psu.edu/Carina_public/special_issue.html through 2011 at
leas
Superior methanol electrooxidation performance of (110)-faceted nickel polyhedral nanocrystals
We present the synthesis of (110)-faceted nickel polyhedral nanocrystals (NCs) and their characterization as electrocatalysts for the methanol oxidation reaction (MOR). Ni NCs were produced at 180 °C through the reduction in solution of a Ni salt. They were combined with carbon black and Nafion and deposited over glassy carbon to study their electrocatalytic properties. Electrodes based on (110)-faceted Ni NCs displayed a first order reaction with KOH in the concentration range from 0.1 M to 1.0 M. These electrodes were characterized by higher coverages of active species, but lower diffusion coefficients of the species limiting the reaction rate when compared with electrodes prepared from spherical Ni NCs. Overall, electrodes based on faceted Ni NCs displayed excellent performance with very high current densities, up to 61 mA cm-2, and unprecedented mass activities, up to 2 A mg-1, at 0.6 V vs. Hg/HgO in 1.0 M KOH containing 1.0 M methanol. These electrodes also displayed a notable stability. While they suffered an activity loss of ca. 30% during the first 10 000 s of operation, afterward activity stabilized at very high current densities, ~35 mA cm-2, and mass activities, ~1.2 A mg-1, with only a 0.5% decrease during operation from 20 000 to 30 000 s.Peer ReviewedPostprint (author's final draft
The Identification of Novel Protein-Protein Interactions in Liver that Affect Glucagon Receptor Activity
Glucagon regulates glucose homeostasis by controlling glycogenolysis and gluconeogenesis in the liver. Exaggerated and dysregulated glucagon secretion can exacerbate hyperglycemia contributing to type 2 diabetes (T2D). Thus, it is important to understand how glucagon receptor (GCGR) activity and signaling is controlled in hepatocytes. To better understand this, we sought to identify proteins that interact with the GCGR to affect ligand-dependent receptor activation. A Flag-tagged human GCGR was recombinantly expressed in Chinese hamster ovary (CHO) cells, and GCGR complexes were isolated by affinity purification (AP). Complexes were then analyzed by mass spectrometry (MS), and protein-GCGR interactions were validated by co-immunoprecipitation (Co-IP) and Western blot. This was followed by studies in primary hepatocytes to assess the effects of each interactor on glucagon-dependent glucose production and intracellular cAMP accumulation, and then in immortalized CHO and liver cell lines to further examine cell signaling. Thirty-three unique interactors were identified from the AP-MS screening of GCGR expressing CHO cells in both glucagon liganded and unliganded states. These studies revealed a particularly robust interaction between GCGR and 5 proteins, further validated by Co-IP, Western blot and qPCR. Overexpression of selected interactors in mouse hepatocytes indicated that two interactors, LDLR and TMED2, significantly enhanced glucagon-stimulated glucose production, while YWHAB inhibited glucose production. This was mirrored with glucagon-stimulated cAMP production, with LDLR and TMED2 enhancing and YWHAB inhibiting cAMP accumulation. To further link these interactors to glucose production, key gluconeogenic genes were assessed. Both LDLR and TMED2 stimulated while YWHAB inhibited PEPCK and G6Pase gene expression. In the present study, we have probed the GCGR interactome and found three novel GCGR interactors that control glucagon-stimulated glucose production by modulating cAMP accumulation and genes that control gluconeogenesis. These interactors may be useful targets to control glucose homeostasis in T2D
A Close Nuclear Black Hole Pair in the Spiral Galaxy NGC 3393
The current picture of galaxy evolution advocates co-evolution of galaxies
and their nuclear massive black holes (MBHs), through accretion and merging.
Quasar pairs (6,000-300,000 light-years separation) exemplify the first stages
of this gravitational interaction. The final stages, through binary MBHs and
final collapse with gravitational wave emission, are consistent with the
sub-light-year separation MBHs inferred from optical spectra and
light-variability of two quasars. The double active nuclei of few nearby
galaxies with disrupted morphology and intense star formation (e.g., NGC 6240
and Mkn 463; ~2,400 and ~12,000 light-years separation respectively)
demonstrate the importance of major mergers of equal mass spirals in this
evolution, leading to an elliptical galaxy, as in the case of the double radio
nucleus (~15 light-years separation) elliptical 0402+379. Minor mergers of
galaxies with a smaller companion should be a more common occurrence, evolving
into spiral galaxies with active MBH pairs, but have hitherto not been seen.
Here we report the presence of two active MBHs, separated by ~430 light-years,
in the Seyfert galaxy NGC 3393. The regular spiral morphology and predominantly
old circum-nuclear stellar population of this galaxy, and the closeness of the
MBHs embedded in the bulge, suggest the result of minor merger evolution.Comment: Preprint (not final) version of a paper to appear in Natur
A Chandra ACIS Study of the Young Star Cluster Trumpler 15 in Carina and Correlation with Near-infrared Sources
Using the highest-resolution X-ray observation of the Trumpler 15 star
cluster taken by the Chandra X-ray Observatory, we estimate the total size of
its stellar population by comparing the X-ray luminosity function of the
detected sources to a calibrator cluster, and identify for the first time a
significant fraction (~14%) of its individual members. The highest-resolution
near-IR observation of Trumpler 15 (taken by the HAWK-I instrument on the VLT)
was found to detect most of our X-ray selected sample of cluster members, with
a K-excess disk frequency of 3.8+-0.7%. The near-IR data, X-ray luminosity
function, and published spectral types of the brightest members support a
cluster age estimate (5-10 Myr) that is older than those for the nearby
Trumpler 14 and Trumpler 16 clusters, and suggest that high-mass members may
have already exploded as supernovae. The morphology of the inner ~0.7 pc core
of the cluster is found to be spherical. However, the outer regions (beyond 2
pc) are elongated, forming an `envelope' of stars that, in projection, appears
to connect Trumpler 15 to Trumpler 14; this morphology supports the view that
these clusters are physically associated. Clear evidence of mass segregation is
seen. This study appears in a Special Issue of the ApJS devoted to the Chandra
Carina Complex Project (CCCP), a 1.42 square degree Chandra X-ray survey of the
Great Nebula in Carina.Comment: Accepted for the ApJS Special Issue on the Chandra Carina Complex
Project (CCCP), scheduled for publication in May 2011. All 16 CCCP Special
Issue papers are available at
http://cochise.astro.psu.edu/Carina_public/special_issue.html through 2011 at
least. 30 pages; 8 figures; 3 table
Study of Bc --> J/psi pi, etac pi decays with perturbative QCD approach
The Bc --> J/psi pi, etac pi decays are studied with the perturbative QCD
approach. It is found that form factors and branching ratios are sensitive to
the parameters w, v, f_J/psi and f_etac, where w and v are the parameters of
the charmonium wave functions for Coulomb potential and harmonic oscillator
potential, respectively, f_J/psi and f_etac are the decay constants of the
J/psi and etac mesons, respectively. The large branching ratios and the clear
signals of the final states make the Bc --> J/psi pi, etac pi decays to be the
prospective channels for measurements at the hadron collidersComment: 21 pages, revtex
Superior methanol electrooxidation performance of (110)-faceted nickel polyhedral nanocrystals
We present the synthesis of (110)-faceted nickel polyhedral nanocrystals (NCs) and their characterization as electrocatalysts for the methanol oxidation reaction (MOR). Ni NCs were produced at 180 °C through the reduction in solution of a Ni salt. They were combined with carbon black and Nafion and deposited over glassy carbon to study their electrocatalytic properties. Electrodes based on (110)-faceted Ni NCs displayed a first order reaction with KOH in the concentration range from 0.1 M to 1.0 M. These electrodes were characterized by higher coverages of active species, but lower diffusion coefficients of the species limiting the reaction rate when compared with electrodes prepared from spherical Ni NCs. Overall, electrodes based on faceted Ni NCs displayed excellent performance with very high current densities, up to 61 mA cm, and unprecedented mass activities, up to 2 A mg, at 0.6 V vs. Hg/HgO in 1.0 M KOH containing 1.0 M methanol. These electrodes also displayed a notable stability. While they suffered an activity loss of ca. 30% during the first 10000 s of operation, afterward activity stabilized at very high current densities, ∼35 mA cm, and mass activities, ∼1.2 A mg, with only a 0.5% decrease during operation from 20000 to 30000 s
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