348 research outputs found

    Pest management and biodiversity in organic fruit production: the case of apple orchards

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    Numerous pesticide applications are required for orchard protection, regardless of the guidelines. Organic fruit production (OFP) mainly relies on the use of mineral fungicides and microbiological or naturally-occurring insecticides. The environmental impact of this type of production does not significantly differ from that of conventional production when assessed in terms of synthetic indicators. However, the abundance of earthworms, as well as the abundance and specific richness of arthropod pests and beneficials in the orchards and surrounding hedges, is greater in OFP than in conventional orchards. Generalist predators are usually less affected by OFP compounds than by the chemical pesticides applied in conventional orchards. OFP also benefits avian communities, and above all, insectivorous birds, for which organic orchards offer a suitable habitat similar to that of undisturbed natural areas. In addition to this general trend, discrepancies may be observed in the protection responses of different insect groups. The abundance of hymenopteran parasitoids is the lowest in organic orchards in which outbreaks of phytophagous mites are also recorded in relation to the intensive use of sulphur for scab protection. Biological insecticides often act in ways that are similar to those of chemical ones, and the restricted choice of available compounds is likely to induce resistance selection in insect pests. Although maintaining biodiversity is not a direct result of the implementation of OFP guidelines, it seems to be widely considered as an option by organic growers, both alone and as a complementary tool for pest regulation

    Flat Low-Loss Silicon Gradient Index Lens for Millimeter and Submillimeter Wavelengths

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    We present the design, simulation, and planned fabrication process of a flat high resistivity silicon gradient index (GRIN) lens for millimeter and submillimeter wavelengths with very low absorption losses. The gradient index is created by sub wavelength holes whose size increases with the radius of the lens. The effective refractive index created by the subwavelength holes is constant over a very wide bandwidth, allowing the fabrication of achromatic lenses up to submillimeter wavelengths. The designed GRIN lens was successfully simulated and shows an expected efficiency better than that of a classic silicon plano-concave spherical lens with approximately the same thickness and focal length. Deep reactive ion etching (DRIE) and wafer-bonding of several patterned wafers will be used to realize our first GRIN lens prototype

    Ancient DNA of guinea pigs (Cavia spp.) indicates a probable new center of domestication and pathways of global distribution

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    Guinea pigs (Cavia spp.) have a long association with humans. From as early as 10,000 years ago they were a wild food source. Later, domesticated Cavia porcellus were dispersed well beyond their native range through pre-Columbian exchange networks and, more recently, widely across the globe. Here we present 46 complete mitogenomes of archaeological guinea pigs from sites in Peru, Bolivia, Colombia, the Caribbean, Belgium and the United States to elucidate their evolutionary history, origins and paths of dispersal. Our results indicate an independent centre of domestication of Cavia in the eastern Colombian Highlands. We identify a Peruvian origin for the initial introduction of domesticated guinea pigs (Cavia porcellus) beyond South America into the Caribbean. We also demonstrate that Peru was the probable source of the earliest known guinea pigs transported, as part of the exotic pet trade, to both Europe and the southeastern United States. Finally, we identify a modern reintroduction of guinea pigs to Puerto Rico, where local inhabitants use them for food. This research demonstrates that the natural and cultural history of guinea pigs is more complex than previously known and has implications for other studies regarding regional to global-scale studies of mammal domestication, translocation, and distribution.Fil: Lord, E.. Stockholms Universitet; Suecia. University of Otago; Nueva ZelandaFil: Collins, C.. University of Otago; Nueva ZelandaFil: deFrance, S.. University of Florida; Estados UnidosFil: LeFebvre, M. J.. University of Florida; Estados UnidosFil: Pigière, F.. Universidad de Dublin; IrlandaFil: Eeckhout, P.. Université Libre de Bruxelles; BélgicaFil: Erauw, C.. Université Libre de Bruxelles; BélgicaFil: Fitzpatrick, S. M.. State University of Oregon; Estados UnidosFil: Healy, P. F.. Trent University; CanadáFil: Martínez Polanco, M. F.. Muséum National d'Histoire Naturelle; Francia. Universitat Rovira I Virgili; España. Institut Català de Paleoecologia Humana i Evolució Social; EspañaFil: Garcia, J. L.. Stetson University; Estados UnidosFil: Ramos Roca, E.. Universidad de los Andes. Facultad de Ciencias Sociales. Departamento de Antropología; ColombiaFil: Delgado Burbano, Miguel Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Área Antropológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. School of Life Sciences and Human Phenome Institute Fudan University; ChinaFil: Sánchez Urriago, A.. Instituto Colombiano de Antropología e Historia; ColombiaFil: Peña Léon, G. A.. Universidad Nacional de Colombia; ColombiaFil: Toyne, J. M.. University of Florida; Estados UnidosFil: Dahlstedt, A.. Arizona State University; Estados UnidosFil: Moore, K. M.. State University of Pennsylvania; Estados UnidosFil: Laguer Diaz, C.. University of Florida; Estados UnidosFil: Zori, C.. Baylor University; Estados UnidosFil: Matisoo-Smith, E.. University of Otago; Nueva Zeland

    Regulation of DNA Methylation Patterns by CK2-Mediated Phosphorylation of Dnmt3a

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    DNA methylation is a central epigenetic modification that is established by de novo DNA methyltransferases. The mechanisms underlying the generation of genomic methylation patterns are still poorly understood. Using mass spectrometry and a phosphospecific Dnmt3a antibody, we demonstrate that CK2 phosphorylates endogenous Dnmt3a at two key residues located near its PWWP domain, thereby downregulating the ability of Dnmt3a to methylate DNA. Genome-wide DNA methylation analysis shows that CK2 primarily modulates CpG methylation of several repeats, most notably of Alu SINEs. This modulation can be directly attributed to CK2-mediated phosphorylation of Dnmt3a. We also find that CK2-mediated phosphorylation is required for localization of Dnmt3a to heterochromatin. By revealing phosphorylation as a mode of regulation of de novo DNA methyltransferase function and by uncovering a mechanism for the regulation of methylation at repetitive elements, our results shed light on the origin of DNA methylation patterns
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