Acessos de mandioca açucarada coletados no Nordeste paraense possuem o mesmo genótipo detectado por marcadores microssatélites.

A mandioca é encontrada na região Norte do Brasil e é usada como fonte de subsistência para muitas famílias. A mandioca açucarada ou mandiocaba, é um tipo de mandioca que armazena em suas raízes, além do amido, açúcares livres como reservas. Atualmente, vêm recebendo destaque pelo seu potencial para uso na fabricação de álcool combustível, devido ao acúmulo de glicose que pode ser convertida em etanol diretamente. Foi realizado teste para avaliar a diversidade genética de acessos de mandiocas açucaradas coletadas em diferentes locais do estado do Pará mantidos no banco de germoplasma da Embrapa Amazônia Oriental. Foi realizada a genotipagem molecular de nove acessos de mandioca açucarada e um acesso de mandioca brava com marcadores microssatélites. Para isso, foram utilizados 13 primers microssatélites e a corrida dos produtos amplificados foi realizada em géis de poliacrilamida 6%. Após a análise dos géis, foi verificado que todas as nove amostras de mandioca açucarada possuíam o mesmo genótipo, sendo verificada baixa variabilidade genética para esse grupo de mandiocas

Variabilidade genética em acessos de mandioca amarela coletadas na Amazônia.

A Amazônia brasileira é conhecida como fonte de variabilidade genética da mandioca. Além disso, costumes locais privilegiaram a conservação de materiais com raiz amarela, rico em carotenóides. O objetivo deste trabalho foi caracterizar uma amostra dos acessos de raiz amarela presentes no BAG da Embrapa Amazônia Oriental. Foram selecionados 18 acessos de mandioca pertencentes ao BAG, sendo 14 materiais com raiz amarela e quatro com raiz branca, por meio de sete locos microssatélites. Todos os locos foram polimórficos, sendo obtido um total de 42 alelos, com média de seis alelos por loco. Quando se avaliou somente as mandiocas amarelas, foram amplificados 36 alelos, com média de 5,12 alelos por loco. A diversidade genética, medida pela heterozigosidade esperada para o grupo das mandiocas amarelas foi de 0,62. O dendrograma não mostrou separação de grupos para as mandiocas, de raiz amarela e raiz branca. No geral, observou-se que é grande a variabilidade genética existente para os materiais de raiz amarela mantidos no BAG da instituição.PIBIC-2011

Short communication: Prevalence of deleterious variants causing recessive disorders in Italian Chianina, Marchigiana and Romagnola cattle.

In the last two decades, the molecular cause of six monogenic autosomal recessive disorders has been identified in native Italian beef cattle: two different ATP2A1 variants for the pseudomyotonia congenita, the first in Chianina and Romagnola (PMT1) and the second in Romagnola (PMT2); a KDM2B variant for the paunch calf syndrome (PCS) in Marchigiana and Romagnola; a NID1 variant for the congenital cataract (CC) in Romagnola; a LAMB1 variant for the hemifacial microsomia (HFM) in Romagnola; an ABCA12 variant for the ichthyosis fetalis (IF) in Chianina and a FA2H variant for the ichthyosis congenita (IC) in Chianina. The aim of this study was to evaluate the potential impact of these disorders in the affected Italian populations. For this purpose, 3331 Chianina, 2812 Marchigiana and 1680 Romagnola bulls born in the last 40 years were considered. The allelic frequency (AF) of the variant for PMT1 was 1.0% in Romagnola, 4.6% in Marchigiana and 5.9% in Chianina. The AF of the variant for PMT2 was 3.3% in Romagnola and 0% in the other two breeds. The AF of the variant for PCS was 11.7% in Romagnola, 2.0% in Marchigiana and 0% in Chianina. The AF of the variants for CC, HFM, IF and IC resulted below 3%, being the variants detected only in the breed populations in which they were previously reported. Considering a selected male population in the single breed, Chianina showed carrier prevalence of 11.9% for PMT1, 7.7% for IC and 6.4% for IF. Romagnola showed carrier prevalence of 23.4% for PCS, 6.7% for PMT2, 4.1% for HFM, 3.2% for CC and 2.0% for PMT1. Marchigiana showed carrier prevalence of 9.1% for PMT1 and 4.0% for PCS. With respect to the Romagnola cattle, the concerning presence of a total of five defect alleles in the population hampers a general approach based on the prevention of carriers from artificial insemination. However, identification of carriers may allow conscious mating to prevent the risk of homozygous descendants as well as the spread of heterozygous offspring. Therefore, systematic genotyping for all seven known harmful alleles is recommended to prevent risk mating between carriers, in particular to avoid the occurrence of affected offspring

Item response theory analysis of the recoded Internet Gaming Disorder Scale-Short-Form (IGDS9-SF)

Based on the nine criteria for Internet gaming disorder (IGD) in DSM-5, the Internet Gaming Disorder Scale 9-Short Form (IGDS9-SF; Pontes and Griffiths 2015) is the most widely used questionnaire for assessing IGD. The present study examined support for the unidimensional factor structure of the instrument, with a group of 868 adolescent and adult gamers from the USA, with criteria recoded as present or absent. The two-parameter logistic model (2PLM) was used to examine the item response theory properties of the criteria included in the measure. Confirmatory factor analysis supported the one-factor model. The 2PLM analysis indicated that all the criteria were strong discriminators of high and low latent IGD. Furthermore, the items measured more of the GAD dimension and with more precision from around +2 SD from the mean trait level. The implications of the findings for interpreting the IGDS9-SF scores for clinical practice are discussed

Antimicrobial activity of fruit packages coated with nanomaterials based in metal-ions zeolites

The transport and storage of fruits is frequently compromised by microbial contaminations, especially during the delivery of these perishable foods to the public. The development of smart packages against microbial contaminations is an alternative of great interest to help controlling post-harvest diseases. The incorporation of nanomaterials, such as metal-ions zeolites with antimicrobial properties, in the semi-rigid alveolar structure for fruits transport/storage could allow the preservation of fruit with safety and quality, by inhibiting/reducing the growth of microrganisms. In this work, metal-ions zeolite nanomaterials were incorporated on the packaging material and the antibacterial activity was tested against Escherichia coli and Staphylococcus aureus. The package material exhibited good antimicrobial activity against the two bacteria (Figure 1), which are promising results for industrial application.info:eu-repo/semantics/publishedVersio

Design and industrial implementation of a multi-functional part produced by powder bed fusion

The use of robot end-effectors programmed to perform handling tasks in industrial environments has expanded in several sectors as a consequence of the rapid digital transformation seen in recent years. With such growing demands, additive manufacturing is evolving in the robotics field with more efficient, innovative, and customer-specific solutions owing to its design flexibility. Thus, this work presents a robot end-effector developed and produced by powder bed fusion (PBF) to execute palletizing operations in the automotive electronics industry, where electrostatic discharge interference is a critical concern. To guarantee the functionality of the developed robot end-effector under real conditions of implementation, prototypes of polyamide 12 (PA12) and PA12 incorporating graphene nanoplatelets were fabricated and characterized. This included the assessment of the mass, electrical surface resistance, and mechanical properties under tensile and compressive loads using biaxial strain gauges coupled to the prototypes for local strain monitoring. The experimental data were then used to define multilinear isotropic hardening models needed to numerically characterize the robotic system. At last, the final product was validated in an industrial environment at Bosch Car Multimedia S.A. Through the applied methodology, valuable insights in guiding process parameters optimization and materials selection for functional PBF parts depending on the technical requirements demanded by industrials are provided.FCT - Fundação para a Ciência e a Tecnologia(2020.04520) / Foundation for Science and Technology (FCT) through the PhD scholarship 2020.04520.BD.European Regional Development Fund through the Operational Competitiveness and Internationalization Programme (COMPETE 2020) [Project No. 47108“SIFA”; Funding Reference: POCI-01-0247-FEDER-04710